【作者】 丁磊；

【导师】 胡志强；

【作者基本信息】 青岛科技大学 ， 有机化学， 2010， 硕士

【摘要】 荧光分子探针具有诸如最高可达单分子检测的高灵敏度、能够实现开关操作、原位检测以及利用光纤维进行远距离检测等众多优点,己经在金属离子、核酸、细胞检测及免疫检测等方面发挥了重要作用。本论文综述了各种类型的阳离子荧光化学传感器,对它们的识别和光化学传感性能作了较详细的介绍。在此基础上,本论文以罗丹明B和蒽环荧光基团为原料合成了一系列的荧光受体2-1~2-4、3-1。并通过核磁共振氢谱、碳谱、元素分析等分析测试手段确定了它们的结构。通过荧光光谱法和紫外-可见光谱法研究了各受体与不同金属离子的相互作用。结果表明,受体2-2对Hg2+、2-3对Cu2+、2-4对Fe3+、3-1对Cu2+具有很好的选择性和识别配位性能,并且,化合物2-1可以在乙腈体系中识别Pb2+,在有水环境中识别Hg2+,是第一次合成的可以分别在1nM和10nM级别上检测Pb2+和Hg2+的化学荧光传感器。通过核磁共振氢谱和质谱研究了各受体与阳离子的作用机制,并对它们的络合方式进行了初步推测。更多还原

【Abstract】 The advantages of molecular fluorescent Probes for sensing can be summarized: high sensitivity of detection down to the single molecule,‘on-off’, switch ability, observation insitu, remote sensing by using optical fibres, et al, and already has played an important role in metal cations, mucleic acids, immune cells and testing.In this thesis, receptors bearing various metal ions binding sites chemosensors were reviewed. Their abilities to selectively bind and sense cations were detailed presented. On the basis, a series of receptors2-1~2-4、3-1 were synthesized from Rhodamine B and anthracene and identified by 1H NMR,13C NMR, and elemental analysis.The interaction between the receptors and various metal cations was studied by fluorescence and UV-vis spectrum. The results demonstrate that receptors 2-2~2-4、3-1 had an excellent selective recognition and sensitivity for Hg2+、Cu2+、Fe3+、Cu2+ respectively, moreover, 2-1 is the first chemosensor based on small molecule that can detect both Pb2+ and Hg2+ at 1nM and 10nM level, respectively.The mechanism of the receptors with cations were studied through 1H NMR and MS, and preliminarly infered the mode of action between them.更多还原