【作者】 李春红；

【导师】 叶云；

【作者基本信息】 泸州医学院 ， 药剂学， 2010， 硕士

【摘要】 目的：细毡毛忍冬(Lonicera similis Hemsl)为忍冬属植物细毡毛忍冬的干燥花蕾或初开的花(习称长吊子银花)。具有抗菌消炎、清热解毒、凉散风热的功效,主要用于治疗痈肿疔疮、喉痹、丹毒、热毒血痢、风热感冒、温热发病等疾病。其主要有效成分为绿原酸、异绿原酸、总黄酮。细毡毛忍冬为川渝产金银花的主流品种。本课题将对川渝产细毡毛忍冬的生药学特征进行研究,对其进行薄层鉴别,并进行有效成分的含量测定,同时对其进行指纹图谱的研究,来提高和完善川渝产细毡毛忍冬的质量控制方法。方法：为了对川渝产细毡毛忍冬的质量标准进行全面控制,本课题采用原植物鉴定、性状鉴定、显微鉴定等方法对川渝产细毡毛忍冬进行生药学鉴别,采用薄层色谱法对绿原酸进行定性鉴别,采用紫外分光光度法测定了总黄酮和总绿原酸的含量,采用高效液相色谱法测定了绿原酸的含量,并采用高效液相色谱法对其指纹图谱进行了研究,为开发利用川渝产细毡毛忍冬提供科学依据。结果：原植物为多年生半常绿木质藤本,幼枝、叶柄和总花梗均被开展的淡黄褐色长糙毛、短柔毛和稀疏腺毛,双花单生于叶腋或少数集生枝端成总状花序。药材具有花蕾细棒槌状,表面为黄棕色、红棕色或绿色,非腺毛较稀疏,腺毛较多的特征。花粉显微特征具有两种腺毛细胞,厚壁非腺毛细胞,薄壁非腺毛单细胞,花粉粒：类球形,具3孔沟,还有棱角细尖的草酸钙簇晶。在薄层色谱中能检测到绿原酸；采用紫外分光光度法测定总黄酮和总绿原酸,测得川银花中总黄酮在0.01～0.08 mg/mL范围内具有良好的线性关系(R=0.9998),回收率为97.71%,RSD为1.48%。采用HPLC测定绿原酸,在0.8-8μg范围内具有良好的线性关系(r=0.9999),平均回收率为98.34%,RSD为0.91%。采用高效液相色谱法进行指纹图谱研究,测得药材有11个共有峰,共有峰的参数符合“中药注射剂指纹图谱技术要求”的有关规定。结论：薄层鉴别显示细毡毛忍冬中含有绿原酸成分；紫外分光光度法测定其总黄酮和总绿原酸含量,方法操作简便、快速、灵敏、具有良好的重复性和回收率,可作为细毡毛忍冬总黄酮和总绿原酸的含量测定方法。高效液相色谱法测定绿原酸的含量,方法操作简便、快速、具有良好的重复性和回收率,可作为川渝产细毡毛忍冬中绿原酸的含量测定方法。采用高效液相色谱法进行指纹图谱研究,方法准确,重复性好,建立的指纹图谱检测标准,可作为细毡毛忍冬质量评价与品种鉴别的重要依据之一。更多还原

【Abstract】 objective:Lonicera similis Hemsl from sichuan and chongqing are dry bud or with The flowers open in early.It belongs Lonicera macranthoides Hand.-Mazz. And Lonicera hypoglauca Miq.and Lonicera confuse DC. Of Caprifoliaceae plants.The uses of which includes Antibacterial anti-inflammatory, heat detoxification, cold wind scattered the effect of heat.It is mainly used for the treatment of carbuncle swollen boils, Houbi, erysipelas, fever sera dysentery, Fengreganmao, incidence of diseases such as warm.Its main effective part is Chlorogenic acid, Iso-chlorogenic acid and total flavonoids and et. Lonicera similis Hemsl are the mainstream varieties of Flos Lonicerae from sichuan.This issue will study the Pharmacognostical features,and it will identificate Lonicera similis Hemsl by TLC, and determinate its effective components, while fingerprinting studies them to enhance and improve the quality of Lonicera similis Hemsl from sichuan and chongqing. Method:For controling the quality standards of Lonicera similis Hemsl from sichuan and chongqing fully, This study using the original plant identification, trait identification, microscopic identification and chemical identification (TLC) method to make a Pharmacognosy Identification.This paper qualitative identificates chlorogenic acid in Lonicera similis Hemsl from sichuan and chongqing using TLC,and determines the total chlorogenic acid and total flavonoids content by ultraviolet spectrophotometry. While using high-performance liquid chromatography (HPLC) to carry out the content of chlorogenic acid, and using HPLC to research its fingerprint. Those could provide a scientific basis for development and utilization of Lonicera similis Hemsl from sichuan and chongqing.Results:The original plants are semi-evergreen and perennial woody plant vines. Young’branches, petiole, and peduncle length were carried out in short brown coarse hair, short hair and sparse hairs soft. Double flowers grow solitary and axillary or terminal branches into a few clusters of racemes. Herbs have the features of small wooden club-shaped flower, yellow brown surface, red brown or green, non-glandular hairs and scattered glandular hairs. Pollen microscopic characteristics with the two kinds of glandular cells, thick-walled non-glandular cells, thin-walled single-celled non-glandular hairs, pollen grains are class spherical, with three-hole groove, there are sharp edges and corners small clusters of calcium oxalate crystal. Using thin-layer chromatography identificates of the legal nature of chlorogenic acid,chlorogenic acid can be detected In the thin-layer chromatography.Using ultraviolet spectrophotometry to detect total flavonoids and total chlorogenic acid, Total flavonoids can be measured in 0.01-0.08 mg/mL with good linearity within the scope relationship (R=0.9998), recovery was 97. 71%, and RSD was 1.48%. Using HPLC to determinate chlorogenic acid,while in the range of 0.8-8μg it has good linear relationship (r=0.9998), the average recovery was 98.34%, and RSD was 0.91%.Using HPLC to carry out fingerprint study.It have been measured a total of 11 peaks,and the parameters of a total of peak accord with "traditional Chinese medicine injection fingerprinting requirements" of the relevant provisions. Conclusion:The TLC show Lonicera similis Hemsl from sichuan and chongqing contains chlorogenic acid composition; Ultraviolet spectrophotometry detecting their total flavonoids and total chlorogenic acid content. The method is simple, rapid, sensitive and with good repeatability and recovery.It can be used as the content of total flavonoids and total chlorogenic acid determination of Lonicera similis Hemsl from sichuan and chongqing. Using HPLC to determinate chlorogenic acid, This method is simple, rapid,efficient, sensitive, accurate and with good repeatability and recovery, it can be used as a quantitative analysismethod for Lonicera similis Hemsl from sichuan and chongqing. Using HPLC carried out its fingerprint study,and the method is accurate and reproducible.The established standards of fingerprints can be used as fine macranthoin of quality evaluation and an important basis for identification of species.更多还原