【作者】 朱庆；

【导师】 倪光夏；

【作者基本信息】 南京中医药大学 ， 针灸推拿学， 2010， 硕士

【摘要】 目的：观察针药结合方法对脑缺血再灌注大鼠脑组织中重要凋亡启动蛋白酶的基因表达的影响,探讨针药结合拮抗脑缺血再灌注后神经元凋亡的核心机制。为临床上采用针药结合疗法治疗脑梗死提供可参考的理论基础和客观依据。方法：以局灶性脑缺血再灌注大鼠模型为研究对象,以针药结合为干预手段(针药结合组),并与单纯针刺干预(针刺组)和单纯药物干预(药物组)进行对照,观察干预治疗前后模型大鼠神经功能评分的变化,同时采用Realtime PCR方法检测各组大鼠在干预治疗前后海马中重要凋亡启动蛋白酶Caspase-9的mRNA的表达变化情况。结果：干预后神经功能评分显示针刺组、药物组、针药组分值均低于模型组,差异有显著性(p<0.05),且以针药组为最优(P<0.01),但针刺组与药物组比较差异无显著性(p>0.05)；3个治疗组均能明显抑制Caspase-9的mRNA的表达,其中针药结合组优于其它组。结论：1.针药结合是治疗缺血性脑血管病的有效手段,疗效优于单纯针刺和单纯药物。2.针药结合可通过更显著地抑制促凋亡蛋白酶Caspase-9的mRNA的表达而抑制受损脑组织的神经细胞凋亡。这可能是针药结合取得良好疗效的重要机制。更多还原

【Abstract】 Objective:To observe the effect of Combination of Acupuncture and medicine on correlative genes of apoptosis which express in cerebral ischemia rats and provide referred theoretical basis and objective underlies for adopting the Combination of Acupuncture and medicine therapy treats cerebral ischemia in clinic.Method:To focal cerebral ischemia model was examined to combination of Acupuncture and medicine for the interventions (combining group), and with simple acupuncture intervention (acupuncture group) and the pure drug intervention (drug group) were observed before and after treatment intervention model of changes in nerve function score, while using Real-time PCR method to detect the expression of the important apoptotic protein Caspase-9 mRNA.in rats after treatment in the intervention.Result:Neurological scores in acupuncture group, drug group, combining group when after the intervention were significantly lower than the ones in the model group (p<0.05), and combining group inhibited significantly (P<0.01),but there was no significant difference between the acupuncture group and drug group (p>0.05); 3 treatment groups could significantly inhibit the expression of Caspase-9 mRNA in all stages of ischemia, and combining group was better than other groups.Conclusion:1.The method of Acupuncture combined with Medicine is an effective means to the therapy of ischemic cerebrovascular disease and more effective than only the acupuncture or medicines.2.The method of Acupuncture combined with Medicine can inhibit the expression of the pro-apoptotic protein Caspase-9 mRNA, which can restraint the apoptosis of nerve cells of damaged brain tissue. This is may be one of the most important mechanisms of acupuncture combined with medicine has well effective.更多还原