【作者】 汪静；

【导师】 石大兴；

【作者基本信息】 四川农业大学 ， 森林培育， 2010， 硕士

【摘要】 本研究以棣棠花Kerria japonica(L.)DC.当年生幼嫩带芽茎段和叶片为试验材料,通过正交实验设计结合方差分析,探讨了棣棠花离体培养的关键影响因素,存在问题及解决方法,对棣棠花茎段诱导、叶片愈伤组织诱导及壮苗生根、炼苗与移栽等方面进行了研究,以得到最佳培养配方及培养程序,从而为建立棣棠花的快繁体系、工厂化生产提供理论与技术依据。通过组织培养技术研究得出：①棣棠花外植体组织培养最佳取材时间为3月,启动率高,污染率相对其它时期较低,茎段和叶片的启动率分别为83.3%和38.3%,污染率分别为38.4%和41.5%。②棣棠花叶片的最佳0.1%HgCl2灭菌时间为5 min,茎段的最佳0.1%HgCl2灭菌时间为7 min。③初代培养：茎段培养以MS+6-BA0.5 mg·L-1+NAA 0.01 mg·L-1+IBA 0.05mg·L-1为最适宜培养基配方组合,启动率高达91.3%,出芽指数1.7。④继代培养：适宜于芽继代增殖培养的最优组合是1/2 MS+6-BA2.0 mg·L-1+NAA0.1 mg·L-1,增殖倍数达4.3,有效苗数3.2。⑤有效苗的培养：以1/2MS+NAA0.1 mg·L-1为最适宜培养基配方组合,植株生长健壮,有效苗数达89.6%。⑥生根培养：以1/2MS+活性炭2.0 g·L-1+NAA 0.05 mg·L-1+蔗糖20 g·L-1为最适宜培养基配方组合,植株生长健壮,生根率最高,达78.7%,平均根数最佳,为3.9条。⑦移栽与炼苗：炼苗基质以蛭石、珍珠岩、河沙以1：1：2的比例效果最好,成活率较高,达到82.3%,且植株长势良好。更多还原

【Abstract】 The study was mainly to probe an efficient way through tissue and rapid propagation of Kerria japonica (L.)DC. The leafaxles of young branches, the leaves are taken as explants.. For know the better medium and proceeding of culturing Kerria japonica (L.)DC., we use orthogonal experimental design and analysis of variance to establish the technique system of tissue culture. The affected factors of propagation, existing problems in this technique and the corresponding solutions are also described, which provide the theoretical base and technical measures for industrial producing of plantlet.The study of tissue culture technique and the better medium for various stages are as follows:①March is the best season to draw materials, in which the germination rate are 83.3% and 38.3% by stem and leaf, the contaminated rate only are 38.4% and 41.5% by stem, and leaf②During the trial:5 minutes is good t treat the laminae by HgCl2(0.1%) and 7 minutes is good to treat the stem.③Initial medium of stem:MS+6-BA 0.5 mg·L-1+NAA 0.01 mg·L-1+IBA 0.05 mg·L-1in which the germination rate is 91.3%, and the shoot forming index is 1.7.④Proliferating of stems cultivation:The optional proliferating buds culture medium stems and shoots wes:1/2 MS+6-BA 2.0 mg·L-1+NAA 0.1 mg·L-1,in which the multiplication rate is 4.3 and the multiplication of efficiency seedings is 3.2.⑤Efficiency seeding culture:1/2MS+NAA0.1 mg·L-1, in which the rate of efficiency seedings is 89.6%.⑥Rooting culture:1/2MS+AC2.0 g·L-1+NAA 0.05 mg·L-1+Sucros 20 g·L-1 in which the rate of rooting is 78.7%, the quantity of roots is 3.9.⑦Forging seeding transplanting:The transplant medium in vermiculite, perlite and riversand by 1:1:2 is the optimal for. Jacaranda mimosifolia, which the rate of survival is 82.3%.更多还原