【作者】 于伟；

【导师】 王振宇；

【作者基本信息】 东北林业大学 ， 食品科学， 2010， 硕士

【摘要】 在中国鹿茸被称为东北三宝之一,马鹿茸是传统名贵中药,含有多种生理活性物质,在中医中药中有着独特的功效和特殊的地位。黑木耳多糖具有提高机体免疫力、抗肿瘤、降血脂、降血糖、抗菌、抗病毒、抗凝血和抗辐射等生理功能。目前对鹿茸降糖及鹿茸与黑木耳多糖复配的功效国内尚未见报道。本文以马鹿鹿茸和黑木耳为原料,研究了鹿茸提取物,以及鹿茸提取物与黑木耳多糖复合制剂对四氧嘧啶(ALX)型糖尿病小鼠的降糖作用,以及对ALX型糖尿病引起的血脂代谢紊乱的调节作用,本研究可为治疗糖尿药物筛选提供参考。具体研究结果如下：(1)鹿茸提取物(DE)与鹿茸提取物和黑木耳多糖复合制剂(EC)均可以调节糖尿病小鼠的饮食水平,并且鹿茸提取物和黑木耳多糖复合制剂(EC)要优于鹿茸提取物(DE)。(2)鹿茸提取物(DE)与鹿茸提取物和黑木耳多糖复合制剂(EC)均可以通过调节糖尿病小鼠的胰岛素,尿酸、肝(肌)糖元和麦芽糖酶的水平来降低糖尿病小鼠的血糖。与模型对照组比,鹿茸提取物(DE)及鹿茸提取物和黑木耳多糖复合制剂(EC)高剂量组糖尿病小鼠的血糖分别降低了59.07%和59.5%。(3)鹿茸提取物(DE)与鹿茸提取物和黑木耳多糖复合制剂(EC)均可以降低甘油三酯、总胆固醇和低密度脂蛋白含量,提高高密度脂蛋白含量,从而预防糖尿病小鼠并发高血脂症。与模型对照组比,鹿茸提取物高剂量组糖尿病小鼠的甘油三酯、总胆固醇、低密度脂蛋白分别降低了39.77%、18.71%、和47.83%；高密度脂蛋白提高了1.76倍；而鹿茸提取物黑木耳多糖复合制剂(EC)高剂量组糖尿病小鼠的甘油三酯、总胆固醇、低密度脂蛋白分别降低了44.78%、34.05%、和63.77%；高密度脂蛋白提高了1.95倍。这也表明鹿茸提取物黑木耳多糖复合制剂(EC)对控制糖尿病小鼠并发高血脂症的效果要优于鹿茸提取物(DE)。(4)鹿茸提取物(DE)与鹿茸提取物和黑木耳多糖复合制剂(EC)均可以提高糖尿病小鼠体内超氧化物歧化酶和谷胱甘肽过氧化物的活性,降低丙二醛的含量。从而延缓脂质过氧化,并且鹿茸提取物和黑木耳多糖复合制剂(EC)要优于鹿茸提取物更多还原

【Abstract】 Red deer antler is one of the best famous chinese medicine and it has many kinds of physiological alive substance. Auriculari-aceae amylose have the physiological function of improving immunity, anti-tumor, lowering serum lipids, lowering serum glucose, antibacterial, anti-virus, anti-thrombin, and so on. So far, the physiological function of extraction from red deer antler and Auriculari-aceae amylose is not reported in China. The red deer antler and Auriculari-aceae were selected as experiment material, and the regulation function of the extraction on serum glucose and serum lipids of diabetic mice induced by Alloxan were disscussed in this study. The results were as follows:(1) The extraction of red deer antler and the compound extraction of red deer antler and Auriculari-aceae amylose all have the regulation function on bite and sup of the mice, and the effect of compound extraction was batter than that of deer antler extraction.(2) The extraction of red deer antler(DE) and the compound extraction of red deer antler and Auriculari-aceae amylose(EC) all have the effect on lowering serum glucose of diabetic mice by way of regulation their insulin, uric acid, liver starch, and maltose enzyme. Contrasted with model control group, the serum glucose of diabetic mice in high dose team were reduced 59.07% and 59.5% by DE and EC respectively.(3) The extraction of red deer antler(DE) and the compound extraction of red deer antler and Auriculari-aceae amylose(EC) all have the effect on lowering TG, TC, and LDL-C, improving HDL-C. Contrasted with model control group, the TG, TC, and LDL-C of diabetic mice in high dose team were reduced 39.77%,18.71%, and 47.83% by DE respectively, but the HDL-C was improved 1.76 times. Contrasted with model control group, the TG, TC, and LDL-C of diabetic mice in high dose team were reduced 44.78%,34.05%, and 63.77% by EC respectively, but the HDL-C was improved 1.95 times. EC had better effect on controling the levels of serum glucose/liquid and correct its lipid metabolism disorder than that of DE.(4) The extraction of red deer antler(DE) and the compound extraction of red deer antler and Auriculari-aceae amylose(EC) all have the effect on improving the activity of SOD, GSH-Px, and lowering the MDA content. The ability on delaying lipid peroxide of EC was better than that of DE.更多还原