【作者】 魏丽娟；

【导师】 刘俊田； 于津浦；

【作者基本信息】 天津医科大学 ， 肿瘤学， 2010， 硕士

【摘要】 目的：研究吲哚胺2,3-双加氧酶(Indoleamine 2,3-dioxygenase,IDO)在乳腺癌中的表达及相应组织内的CD31、CD105标记的肿瘤微血管密度(microvessel density, MVD),明确乳腺癌中IDO蛋白的表达与临床病理特征的关系及其预后的意义,同时寻找IDO与MVD之间的关系。利用Transwell小室,建立表达IDO的肿瘤细胞MCF-7与人脐静脉内皮细胞HUVEC共培养模型,检测肿瘤细胞分泌的IDO对HUVEC的增殖、CD105表达情况及成管能力的影响,探讨IDO在乳腺癌血管形成过程中发挥的作用及可能机制。方法：1.收集天津医科大学附属肿瘤医院2004年1月手术切除的乳腺癌石蜡切片40例,免疫组织化学法检测IDO、CD31、CD105的表达情况,观察IDO表达与临床病理特征的关系及其预后的意义,同时分析IDO表达与MVD的关系。2.半定量RT-PCR法检测IDO基因的表达情况,筛选表达IDO的乳腺癌细胞系。3.建立表达IDO的人乳腺癌细胞系MCF-7与人脐静脉内皮细胞HUVEC共培养模型,利用IDO抑制剂1-甲基色氨酸(1-MT)阻滞IDO活性,细胞计数法检测MCF-7分泌的IDO作用后HUVEC增殖情况,流式细胞仪检测CD105表达率变化,基质胶实验观察HUVEC形成管样结构能力的变化情况。结果：1.免疫组化结果显示乳腺癌组织中IDO蛋白表达率为67.5%(27/40),低表达或不表达率为32.5%(13/40)。乳腺癌组织中IDO表达水平与患者临床分期和腋窝淋巴结转移密切相关(P<0.05)。2.乳腺癌组织中IDO蛋白高表达组的患者3年、5年无瘤生存率均低于IDO蛋白不表达或低表达组,分别为88.9%(24/27)与100%(13/13)、85.2%(23/27)与92.3%(12/13),但差异无统计学意义。3.乳腺癌组织中IDO表达水平与CD105标记的MVD(MVD-CD105)正相关(rs=0.659,P<0.05),与CD31标记的MVD(MVD-CD31)无关。4.RT-PCR结果显示：人乳腺癌细胞系MDA-MB-231、MDA-MB-435S、MDA-MB-453、SK-Br-3、MCF-7、T47D、ZR-75-1中,MCF-7、MDA-MB-435S、T47D的IDO表达水平较其余细胞系高。5.利用Transwell小室,建立表达IDO的人乳腺癌细胞系MCF-7与人脐静脉内皮细胞HUVEC共培养模型,使用IDO抑制剂1-甲基色氨酸(1-MT)阻滞IDO活性,通过倒置显微镜观察细胞形态,同时利用细胞计数法绘制HUVEC细胞生长曲线发现：MCF-7共培养后HUVEC增殖明显,加入1-MT后这种增殖作用几乎完全被抑制。6.流式细胞检测结果显示：HUVEC细胞表达CD105,48h表达率最高；MCF-7共培养后促进HUVEC细胞表面CD105的表达,48h作用最明显；加入1-MT后CD105的表达率降低。7.基质胶成管实验结果显示：HUVEC可以形成管样结构；MCF-7共培养后,管样结构形成增加,24h作用最明显；加入1-MT组几乎无管样结构形成。结论：1.乳腺癌组织中IDO蛋白表达率为67.5%(27/40),且其表达水平与肿瘤临床分期和腋窝淋巴结转移密切相关(P<0.05),IDO高表达组的3年、5年无瘤生存率均低于IDO不表达或低表达组,但差异无统计学意义。提示肿瘤组织中IDO的表达水平与乳腺癌的进展和转移相关。2.乳腺癌组织中IDO表达与MVD-CD105正相关(rs=0.659,P<0.05),与MVD-CD31无关,提示IDO可能通过促进肿瘤新生血管形成进而加速肿瘤的进展,同时说明CD105标记的肿瘤新生血管可能较CD31所标记的血管对肿瘤转移的意义更大。3.人乳腺癌细胞系MDA-MB-231、MDA-MB-435S、MDA-MB-453、SK-Br-3、MCF-7、T47D、ZR-75-1中,MCF-7、MDA-MB-435S、T47D的IDO表达水平较其余细胞系高。4.肿瘤细胞分泌的IDO可以促进HUVEC的增殖、活化并提高其形成管样结构的能力,进一步验证了IDO对肿瘤组织中新生血管的形成具有一定促进作用。更多还原

【Abstract】 Objective:To investigate the expression of Indoleamine 2,3-dioxygenase (IDO) in breast cancer and its correlation to clinicopathologic factors and prognosis. To investigate the expression of CD31、CD105 which can be used as markers of MVD. To investigate the relationship between IDO and MVD. Furthermore, using a co-culture system of MCF-7 and HUVEC, we will evaluate the effects of IDO on proliferation of HUVEC in vitro. We also detected the CD105 of HUVEC after the co-culturing. Moreover, IDO expression can promote the formation of vessel-like structures in vitro. These findings suggest that IDO may promote angiogenesis in vitro.Methods:1. The paraffin blocks of 40 cases of breast cancer were collected from Tianjin cancer hospital. Immunohistochemistry was used to detect the expression of IDO、CD31 and CD105 proteins in previous tissues, then to seek the relationship between them. We investigated the relationship between IDO and clinicopathologic factors and prognosis.2. RT-PCR was used to detect the mRNA of IDO in human breast cancer cell lines, such as MDA-MB-231、MDA-MB-435S、MDA-MB-453、SK-Br-3、MCF-7、T47D、ZR-75-1.3. Using a co-culture system of MCF-7 and HUVEC, we evaluate the effects of IDO on proliferation、the expression of CD105、the formation of vessel-like structures of HUVEC in vitro.Results:1. The overexpression ratio of IDO in breast cancer is 67.5%(27/40), and expression of IDO was closely associated with clinical stage and lymph nodes metastasis.2. The disease-free survival rate in patients with overexpression of IDO was lower than that in patients with negative or low expression of IDO (P>0.05)3. The expression of IDO was positively correlated with MVD-CD105 (P <0.05). 4. RT-PCR results showed that human breast cancer cell lines MCF-7、MDA-MB-435S、T47D are high expression of IDO. MDA-MB-231、MDA-MB-453、SK-Br-3、ZR-75-1 are low or no expression of IDO.5. Using a co-culture system of MCF-7 and HUVEC, IDO can promote the proliferation、the expression of CD105、the formation of vessel-like structures of HUVEC in vitro.Conclusion:1. The overexpression ratio of IDO in breast cancer is 67.5%.Expression of IDO was associated with clinical stage and lymph nodes metastasis.2. In breast cancer, the expression of IDO may be in relation to increase MVD, which suggested that IDO maybe induced tumor angiogenesis, then to promote cancer cells metastasis.Meanwhile, CD105 is a better marker than CD31 in cancer.3. Using a co-culture system of MCF-7 and HUVEC, we evaluate the effects of IDO on proliferation the expression of CD105、the formation of vessel-like structures of HUVEC in vitro.更多还原