【作者】 程洋；

【导师】 孙丰源；

【作者基本信息】 天津医科大学 ， 眼科学， 2010， 硕士

【摘要】 研究目的通过观察B转化生长因子(TGF-β1)、β转化生长因子Ⅱ型受体(TβRⅡ)及增殖细胞核抗原(PCNA)在泪腺上皮性肿瘤中的表达情况,并分别分析TGF-β1与TβRⅡ、PCNA的相关性,进而探讨TGF-β1信号通路在泪腺上皮性肿瘤发生与发展中的作用,及TGF-β1在泪腺上皮性肿瘤细胞增殖中的生物学作用,为将TGF-β1信号通路引入泪腺上皮性肿瘤的诊断、治疗及预后判断提供客观依据。研究方法1.收集2003年11月至2008年11月期间在天津市眼科医院诊断为泪腺上皮性肿瘤患者的临床病理资料。2.应用免疫组织化学法检测TGF-β1、TβRⅡ及PCNA在泪腺上皮性肿瘤中的表达情况。3.应用SPSS13.0统计学软件对数据进行分析。研究结果1 TGF-β1蛋白主要表达于细胞胞质内,偶见于胞核,其表达与患者年龄、性别、肿瘤大小、发病时间无关。TGF-β1蛋白的平均光密度(AOD)值在正常泪腺组,泪腺良性上皮性肿瘤组及恶性上皮性肿瘤组分别为0.0192±0.0062,0.0857±0.0484,0.2414±0.0751,三组间两两比较有统计学差异(P<0.05)。TGF-β1蛋白的AOD值在良性上皮性肿瘤初发组及复发组中分别为0.0626±0.0213,0.2370±0.0778,两组间比较有统计学差异(P<0.05)；其在恶性肿瘤初发组与复发组中分别为0.2390±0.0743,0.2502±0.0713,两组间比较无统计学差异(P>0.05)。2 TβRⅡ蛋白主要表达于细胞胞质内,其表达与患者年龄、性别、肿瘤大小、发病时间无关。TβRⅡ蛋白在正常泪腺组,泪腺良性上皮性肿瘤组及恶性上皮性肿瘤组间均有表达,其AOD值分别为0.2548±0.0292,0.2498±0.0285,0.2497±0.0314,三组间两两比较无明显统计学差异(P>0.05); TβRⅡ蛋白的AOD值在泪腺良性上皮性肿瘤初发组及复发组中分别为0.2504±0.0301,0.2501±0.0334,两组间比较无统计学差异(P>0.05)；其在恶性肿瘤初发组与复发组中分别为0.2489±0.0279,0.2481±0.0241,两组间比较无统计学差异(P>0.05)。3 PCNA蛋白主要表达于细胞胞核,其表达与患者年龄、性别、肿瘤大小、发病时间无关。PCNA蛋白的平均光密度(AOD)值在正常泪腺组,泪腺良性上皮性肿瘤组及恶性上皮性肿瘤组分别为0.133±0.0213,0.17±0.0234,0.2445±0.0323,三组间两两比较有统计学差异(P<0.05)。PCNA蛋白的AOD值在泪腺良性上皮性肿瘤初发组及复发组中分别为0.1499±0.0468,0.2431±0.0314,两组间比较有统计学差异(P<0.05)；其在恶性上皮性肿瘤初发组与复发组中分别为0.2344±0.0306,0.2647±0.0261,两组间比较有统计学差异(P<0.05)。4.泪腺上皮性肿瘤中TGF-β1与PCNA蛋白表达呈正相关(P<0.05),TGF-β1与TβRⅡ表达无明显相关性(P>0.05)。结论1. TGF-β1参与了泪腺上皮性肿瘤的发生与发展,可预测良性上皮性肿瘤的复发性。2.在泪腺上皮性肿瘤中TβRⅡ无明显表达缺失,其可能不是导致TGF-β1失去抑制细胞增殖作用的重要因素。3. PCNA可以反映泪腺上皮性肿瘤细胞的增殖活性及肿瘤的恶性程度并评价泪腺上皮性肿瘤的预后及生物活性,为临床早期干预提供理论依据。4. TGF-β1随着肿瘤恶性程度的增强,其抑制细胞增殖的作用减弱,可能失去了抑制细胞增殖、调控细胞分化的生物学功能,并且其高表达可能促进了肿瘤细胞的增殖。更多还原

【Abstract】 ObjectiveThis study is to investigate the expressions of TGF-β1, TβRⅡand PCNA in lacrimal gland epithelial tumors,it evaluates the possible role of TGF-β1 signaling pathway in the occurrence and development of lacrimal gland tumor. Simultaneously, it clarifies whether TGF-β1 plays role in cell proliferation by analysis the relevance of TGF-β1 and PCNA.That will be benfit to the diagnosis and treatment,and to evaluate the prognosis of lacrimal gland tumor as a theoretical basis.Methods1. Collect the linical and pathological information of patients diagnosed as lacrimal gland epithelial tumors from November 2003 to November 2008 in Tianjin Eye Hospital.2. Immunohistochemical staining was used to detect the expression of TGF-β1, TβRⅡand PCNA in lacrimal gland epithelial tumors.3. The data was analyzed by SPSS 13.0 statistical software.Results1. TGF-β1, TβRⅡprotein were mainly localized in the cytoplasm, and PCNA protein was localized in cell nuclei,their expressions have nothing to do with ages,sexs, tumor size and course of disease.2. The AOD values of TGF-β1 protein respectively were 0.0192±0.0062,0.0857±0.0484,0.2414±0.0751 in the normal group,benign group and malignant goup,significant difference among them(P<0.05); The AOD values of TGF-β1 protein respectively were 0.0626±0.0213,0.2370±0.0778 in no recurrence group of the benign epithelial tumors and recurrence group,significant difference between them(P<0.05). The AOD values of TGF-β1 protein respectively were 0.2390±0.0743,0.2502±0.0713 in no recurrence group of the malignant epithelial tumors and recurrence group,no difference between them(P>0.05).3. The AOD values of TβRⅡprotein respectively were 0.2548±0.0292,0.2498±0.0285,0.2497±0.0314 in the normal group,benign group and malignant goup, there were no significant differences among them (P> 0.05); The AOD values of TβRⅡprotein respectively were 0.2504±0.0301,0.2501±0.0334 in no recurrence group of the benign epithelial tumors and recurrence group,no difference between them (P> 0.05). The AOD values of TβRⅡprotein respectively were 0.2489±0.0279,0.2481±0.0241 in no recurrence group of the malignant epithelial tumors and recurrence group,no difference between them(P>0.05).4. The AOD values of PCNA protein respectively were 0.133±0.0213,0.17±0.0234,0.2445±0.0323 in the normal group,benign group and malignant goup,significant difference among them(P<0.05); The AOD values of PCNA protein respectively were 0.1499±0.0468,0.2431±0.0314 in no recurrence group of the benign epithelial tumors and recurrence group,significant difference between them(P <0.05). The AOD values of PCNA protein respectively were 0.2344±0.0306,0.2647±0.0261 in no recurrence group of the malignant epithelial tumors and recurrence group, significant difference between them(P<0.05).5. The positive correlation was showed between protein expression levels of TGF-β1 and PCNA in lacrimal gland tumors (P<0.05); protein expression levels of TGF-β1 and TβRⅡwere no significant correlation (P> 0.05).Conclusion1. TGF-β1 participates in the occurrence and development of lacrimal gland tumor,and possibily play a important role in reccurence of benign epithelial tumors.2. TPRⅡprotein possibily was no loss in lacrimal gland tumors, and it had no relationship with TPRⅡprotein that TGF-β1 can not inhibit the cell proliferation.3. PCNA can reflect cell proliferation of lacrimal gland tumor, and evaluate the clinical biological behavior of lacrimal gland tumor.4. TGF-β1 can’t play a biological role of inhibiting the cell proliferation with the increasing of the degree of tumor malignancy and the high expression of TGF-β1 may promote tumor cell to proliferate.更多还原