【作者】 解海杰；

【导师】 徐勇； 张志宏； 杨阔；

【作者基本信息】 天津医科大学 ， 外科学， 2010， 硕士

【摘要】 研究背景：近期,独立的全基因组关联扫描已经证实位于染色体8q24上多个基因变异位点与前列腺癌风险有关联性。rs1447295位点是第一个在两个独立的研究样本中被证实的与前列腺癌风险关联的基因变异位点,并且在欧美人群中显示了很强的关联性。这一关联性已在欧洲裔美国人、美国黑人、日裔美国人、夏威夷本土人、日本人群、亚洲印度人群得到验证。2007年,Gudmundsson等证实了第二个位于8q24上遗传变异位点rs16901979与前列腺癌的关联性,同期,Haiman等发现了5个位于8q24遗传变异位点与前列腺癌关联性,其中rs6983561位点的关联性最强。本研究目的是为了研究3个在欧美人群中与前列腺癌高度关联的基因变异位点rs698356、rs16901979、rs1447295位点,在中国汉族人群中是否与前列腺癌有关联性。方法：通过中国汉族人群120例PCa患者和120例对照人群的病例对照研究,采用直接测序法对rs698356、rs16901979、rs1447295等3个SNP位点基因型分型,分析3个SNP与前列腺癌风险关联性,同时也在不同PSA水平、肿瘤分期、Gleason分级、肿瘤侵袭性亚组间进行关联性分析。结果：(1)rs6983561C等位基因与前列腺癌风险无关联性,OR值为1.19(95%CI=0.81-1.76,P=0.367)。rs6983561位点C等位基因在低分期肿瘤与高分期肿瘤及侵袭性前列腺癌组与前列腺癌风险均有关联性,OR值分别为3.54(95%CI:2.38-5.25;P=0.000), 1.60(95%CI:1.02-2.51;P=0.O40), 1.78 (95%CI:1.16-2.67; P=0.008)。(2) rs16901979A等位基因与前列腺癌风险无关联性,OR值为1.13(95%CI=0.76-1.66, P=0.55)。rs16901979位点A等位基因在不同PSA水平、Gleason评分、肿瘤分期、肿瘤侵袭性组间与前列腺癌风险均无关联性(P均>0.05)。(3)rs1447295A等位基因与前列腺癌风险有关联性,OR值为1.63(95%CI:1.02-2.63, P=0.042)。rs1447295位点A等位基因在高PSA组(>20ng/mL)与侵袭性前列腺癌组与前列腺癌风险有关联性,OR值分别为1.63(95%CI:1.00-2.66; P=0.033),1.63(95%CI:1.00-2.67；P=0.049)。结论：rs1447295位点多态性在中国汉族人群中与前列腺癌有关联性,rs1447295A等位基因携带者能增加前列腺癌的风险。更多还原

【Abstract】 BACKGROUND.Recently, independent genome-wide scans have identified multiple genetic variants at 8q24 to be associated with PCa risk. The rs1447295 was the first variant identified by two independent study groups,and it was shown to have the strongest association in European and African populations. This association was replicated in European Americans, African Americans, Japanese Americans, Native Hawaiians, Japanese,and Asian Indians. Gudmundsson et al identified a second variant rs16901979 at 8q24 associated with PCa risk. At about the same time, Haiman et al identified five previously unidentified risk variants at 8q24, of which rs6983561 showed the strongest association with PCa. Most of the association studies have been performed in American, African and European populations. We performed this study to determine whether these three risk variants at 8q24 were associated with PCa risk in Chinese han population.METHODS. We conducted a case-control study comprising of 120 prostate patients and 120 healthy controls in Chinese han population. Genotyping by direct sequencing was performed for rs16901979, rs6983561and rs1447295. Their association with disease stage, tumor grade, PSA level and disease aggressiveness was also determined.RESULTS.1. The allele C of rs6983561 was not associated with prostate cancer risk, odds ratio was 1.19(95% CI:0.81-1.76,P=0.367). The risk allele C was associated with prostate cancer risk in PCa patients of both stages (A+B) and stages (C+D),and in patients with aggressive disease, odds ratio was 3.54(95% CI:2.38-5.25; P=0.000), 1.60(95% CI;1.02-2.51; P=0.040),1.78 (95% CI:1.16-2.67;P=0.008) respectively.2. The allele A of rs 16901979 was not associated with prostate cancer risk,odds ratio was 1.13(95% CI:0.76-1.66,P=0.55).The allele A of rs16901979 was not associated with prostate cancer risk in PCa patients of all stages, all Gleason scores, all PSA level and all disease aggressiveness groups(P>0.05)3. The A allele of rs 1447295 was significantly associated with prostate cancer risk,odds ratio was 1.63(95% CI:1.02-2.63,P=0.042). The allele A of rs1447295 was associated with PCa risk in patients with PSA levels>20 ng/mL and in patients with aggressive disease,odds ratio was 1.63 (95%CI:1.00-2.66;P=0.033) and 1.63 (95% CI:1.00-2.67;P=0.049) respectively.CONCLUSION. The variants rs1447295 at 8q24 is associated with prostate cancer risk in Chinese han population. The A allele of rs1447295 carrying can increase the risk of prostate cancer.更多还原