【作者】 李波；

【导师】 吴春梅； 管洪在；

【作者基本信息】 青岛大学 ， 临床检验诊断学， 2010， 硕士

【摘要】 目的：探讨染色体分析对慢性骨髓增殖性疾病(CMPD)分类、诊断及疗效判断的临床意义。方法：收集CMPD患者骨髓细胞,采用24h常规培养法及R显带技术制备染色体标本,分析染色体核型改变,并对部分患者进行动态观察。结果：①159例CMPD患者中染色体异常的检出率为69.18%,其中122例初诊为CML患者中,染色体异常的检出率为85.25%(104/122),37例初诊为其他类型的CMPD患者中,染色体异常的检出率为16.22%(6/37)。②CML慢性期与急变期和加速期患者出现额外染色体异常的比例分别为14.42%和61.11%,两者有显著性差异(X2=15.228,P<0.01)。③CML患者应用格列卫治疗与骨髓移植治疗后Ph染色体转阴率没有明显差异(X2=0.0178,P>0.05)。结论：额外染色体异常的出现与CML病人的病程进展密切相关；染色体分析对CMPD的分型、诊断及疗效判断具有重要意义。更多还原

【Abstract】 Objective:To investigate the clinical significance of chromosome analyzing in classification, diagnosis and efficacy to determine with the myeloproliferative disorders (CMPD).Methods:Making a collection of bone marrow cells with CMPD were prepared by short-time culture, karyo-typing was performed by R-banding, dynamic detection of chromosomal changes were carried out in some patients in the course of progress.Results:①The detection rate of chromosomal abnormality in 159 cases of CMPD patients was 69.18%, of which, in 122 cases of CML patients, was 85.25%(104/122), in 37 cases with newly diagnosed other types CMPD patients, the detection rate was 16.22% (6/37).②The rate of extra chromosome was found in 4.42% of patients in chronic phase (CP), and 61.11% in accelerated phase (AP) and blast crisis phase (BP), which had significant difference(X2=15.228, P<0.01).③Ph chromosome negative rate in CML patients with Gleevec therapy compared with bone marrow transplantation after treatment was not significantly different (X2=0.0178, P>0.05).Conclusions:There is a close correlation between complex chromosome abnormalities and the progress of CML. Chromosome analysis plays an important role in the classification, diagnosis, and efficacy to determine of CMPD.更多还原