【作者】 梁瑜；

【导师】 岳旺；

【作者基本信息】 青岛大学 ， 药理学， 2010， 硕士

【摘要】 目的研究壳寡糖对急、慢性痛风的预防作用,探讨其作用机制,以研发防治痛风的天然新药。方法1.用尿酸钠晶体(MSU)溶液注入大鼠右侧踝关节腔制造急性痛风模型。大鼠随机分为正常对照组、模型组、阳性药对照组和预防组(n=8),观察壳寡糖对大鼠急性痛风性关节炎的关节周径、血白细胞数及血清IL-1B的影响。2.用腺嘌呤和乙胺丁醇灌胃法制备大鼠高尿酸血症痛风模型。大鼠随机分为正常对照组、模型组、阳性药对照组和预防组(n=8),观察壳寡糖对大鼠慢性痛风的黄嘌呤氧化酶活性、血尿酸、尿素氮、肌酐水平及肾脏病理的影响。结果1.MSU可诱发大鼠急性痛风性关节炎,壳寡糖对关节肿胀有抑制作用,升高血白细胞数,降低血清IL-1β(P<0.01)。2.腺嘌呤和乙胺丁醇灌胃法可诱导大鼠慢性痛风模型,壳寡糖可降低大鼠黄嘌呤氧化酶活性,尿酸、尿素氮和肌酐水平(P<0.01)；减少肾小管内MSU及炎细胞浸润,减轻肾小管损伤。结论1.壳寡糖在大鼠急性痛风性关节炎模型,可抑制急性痛风性关节炎发作。2.壳寡糖在大鼠高尿酸血症动物模型,可预防慢性痛风。更多还原

【Abstract】 ObjectiveTo investigate the effect of Chitosan oligosaccharide on acute and chronic gout as well as mechanisms in order to develop new natural drug to control gout.Methods1. After an acute gouty arthritis model of rat was established with monosodium urate crystal (MSU), animals were randomly divided into normal control group, model group, positive control group and three prevented groups(n=8). The effect of Chitosan oligosaccharide on ankle perimeters, leucocyte count and IL-1βtest of rats was observed.2. After a chronic gout model of rat was established with adenine and ethambutol, animals were randomly divided into normal control group, model group, positive control group and three prevented groups(n=8). The effect of Chitosan oligosaccharide on activity of XOD, level of UA, BUN and CRE as well as pathological changes of kidney was observed.Results1. An acute gouty arthritis model of rat could be induced by MSU. Chitosan oligochitosan exhibited inhibitive effect on swelling of ankle and increased leucocytes as well as decreasing IL-1βof model rats (P<0.01).2. A chronic gout model of rat was established with adenine and ethambutol. Chitosan oligochitosan could lower activity of XOD and decrease level of UA, BUN and CRE (P<0.01). Monosodium urate crystals, cellular infiltration and damage of kidney tubules were reduced in Chitosan oligochitosan groups.Conclusion1. Chitosan oligochitosan can inhibit the attack of acute gouty arthritis in rats.2. Chitosan oligochitosan can prevent chronic gout in the hyperuricemia model of rats.更多还原