【作者】 李自亨；

【导师】 刘勇；

【作者基本信息】 青岛大学 ， 外科学， 2010， 硕士

【摘要】 目的近年来研究发现,长期服用非甾体类抗炎药(NSAID)有与抗炎作用无关的抗肿瘤功能,至今,其对抗肿瘤的确切机制还未完全明了,本文章主要探讨选择性环氧化酶-2抑制剂塞来昔布对膀胱肿瘤的抑制作用及其机制。方法建立小鼠膀胱肿瘤模型并随机分为对照组、实验1组和实验2组,分别给与生理盐水和不同剂量的塞来昔布灌胃,观察肿瘤的生长情况,测量肿瘤体积、质量并计算抑瘤率；免疫组织化学方法检测肿瘤原癌基因bcl-2和半胱氨酸天门冬氨酸蛋白酶-3(caspase-3)的表达情况并计算其免疫组化评分(HIS)。结果实验1、实验2组肿瘤的平均质量明显低于对照组,差异有显著性(P<0.05)。实验1、实验2组的抑瘤率分别为43%、58%。在bcl-2免疫组化切片中,bcl-2主要表达于细胞浆中,对照组,实验一组,实验二组,免疫组化的颜色逐渐减淡,染色细胞数量减少；而caspase-3也主要表达于细胞浆中,但随着塞来昔布剂量的增加,免疫组化的颜色逐渐加深,所表达的细胞数量也增加3组间bcl-2HIS比较差异有显著性(F=25.08,q=3.75～9.91,P<0.05)；3组间caspase-3的HIS比较差异有显著性(F=16.82,q=3.26～8.15,P<0.05)。结论塞来昔布能抑制小鼠膀胱肿瘤的生长,其机制可能与抑制bcl-2,增强caspase-3的表达有关。该实验为临床中抑制肿瘤的生长的化疗药物提出了新的方向和选择。更多还原

【Abstract】 Objective To detect whether cyclooxygenase-2 inhibitor celecoxib could prevent mice urinary bladder cancer and to explore the mechanisms of the effect.Methods The mouse bladder tumors model was successfully established, then all mice were randomly divided into control group and test 1,2 group. Celecoxib in suspension was administered to mice in the test group orally, while saline to the mice in the control group. During the test, the growth of tumor mass was observed, the volume and weight of transplantation tumor, and the rate of tumor inhibition were detected. The protein expression of bcl-2 and caspase-3 were examined by immunohistochemical method.The immunohistochemical scores (IHS) were calculated by combining an estimate of the percentage of immunoreactive cells with that of the stain intensity.Result The average quality of experiment 1, experimental group 2 tumors were significantly lower than the control group, the difference was significant (P<0.05). The inhibited rates of experiment 1, experimental group 2 were 43%,58%. The difference of bcl-2 HIS was significant among the 3 groups (F=25.08,q=3.75～9.91,P<0.05), The difference of caspase-3 HIS was significant among the 3 groups (F=16.82,q=3.26-8.15,P<0.05).Conclusion Different doses of celecoxib can significantly suppress tumor growth in a dose-dependent manner, it maybe have something with the decrease of bcl-2’expression but the increase of caspase-3’expression.更多还原