【作者】 李宏庆；

【导师】 朱惠莉； 李向阳； 朱砚萍；

【作者基本信息】 复旦大学 ， 内科学， 2010， 硕士

【摘要】 良、恶性胸腔积液的蛋白质组学研究【目的】利用蛋白质组学技术获得并分析良、恶性胸腔积液的蛋白质表达谱,寻找具有诊断意义的蛋白标志物及建立诊断模型,有利于恶性胸腔积液的诊断及良、恶性胸腔积液的鉴别。【方法】收集一组恶性胸腔积液(8例肺腺癌癌性胸腔积液)和一组良性胸腔积液(共7例,4例为炎症性胸腔积液,3例为漏出液),经磁珠纯化(液体蛋白芯片技术)、基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)分析及生物信息学方法获得并研究其蛋白质表达谱。【结果】经良、恶性胸腔积液蛋白质图谱分析比较,找到两个具有显著统计学差异的蛋白,质核比(m／z)分别为3927.49Da及5865.49Da,在恶性胸腔积液中均呈低表达。建立了诊断模型,建模蛋白质为5534.81(m／z)、4963.18(m／z)、2788.87(m／z)。【结论】利用液体蛋白芯片-飞行时间质谱技术对良、恶性胸腔积液进行蛋白质组学研究,获得了良、恶性胸腔积液的蛋白质图谱,得到了差异蛋白和诊断模型,为临床胸腔积液的诊断与鉴别诊断提供了新的思路和方法,差异蛋白质的鉴定结果可能为肺腺癌及恶性胸腔积液的诊断及治疗提供有价值的途径。更多还原

【Abstract】 Objective Use the proteomics technology to acquire and analyze the protein profiles of the benign and malignant pleural effusion, to seek useful protein biomarkers with diagnostic value and to establish the diagnostic model, for diagnosis of the malignant pleural effusion and antidiastole between the benign and malignant pleural effusion.Method Collect one group of malignant pleural effusion (8 samples from 8 lung adenocarcinoma patients) and one group of benign pleural effusion (total 7 samples including 4 inflammatory pleural effusion and 3 transudate), to acquire and analyze their protein profiles by functional magnetic bead based sample fractionation method (liquid protein chip technology) combinded with matrix-assisted laser desorption/ionization—time of flight—mass spectrometry (MALDI-TOF-MS) and bioinformatics method.Results The protein profiles of benign and malignant pleural effusion were analyzed and compared. Two differential proteins with significant statistical difference were got, the mass-to-charge ratio (m/z) were 3927.49Da and 5865.49Da respectively, down-regulated in the malignant pleural effusion. The diagnostic model was established with three proteins which the mass-to-charge ratio (m/z) were 5534.81Da and 4963.18Da and 2788.87 respectively.Conclusion Through the proteomic study of benign and malignant pleural effusion by using liquid protein chip technology combinded with time of flight—mass spectrometry method, we acquired the protein profiles of the benign and malignant pleural effusion, besides, got the differential proteins and diagnostic model. The result provided new thought and method for diagnosis and antidiastole of the malignant pleural effusion in clinics. Furthermore, the identification of differential proteins maybe provide valuable path to the diagnosis and therapy of lung adenocarcinoma and malignant pleural effusion.更多还原