【作者】 黄娅珣；

【导师】 苗雄鹰；

【作者基本信息】 中南大学 ， 普通外科学， 2010， 硕士

【摘要】 目的研究磷脂酰肌醇蛋白聚糖3 (Glypican-3)和原癌基因C-myb在原发性肝癌(Primary Hepatic Carcinoma, PHC)组织中的表达情况,探讨它们之间的相互关系以及它们与原发性肝癌临床病理特征之间的关系。方法收集中南大学湘雅二医院肝胆胰外科2001年1月至2006年12月经手术切除及病理证实的原发性肝癌标本50例(术前均未进行化疗和放疗)及15例癌旁组织(距癌肿≥3cm)为对照,经10%甲醛固定后常规石蜡包埋连续切片,切片厚3μm,应用免疫组化SP法分别检测癌组织及癌旁组织的Glypican-3蛋白和C-myb蛋白的表达情况,并于高倍镜下评分。结果Glypican-3蛋白在原发性肝癌的癌组织中有表达,在癌旁组织中未见表达,C-myb蛋白在原发性肝癌的癌组织和癌旁组织均有表达,且原发性肝癌组织中Glypican-3蛋白和C-myb蛋白的阳性表达率和表达评分与癌旁组织相比均具有显著的统计学差异(P<0.01,P<0.05)。Glypican-3蛋白表达与年龄、性别、肝癌病理类型、病理分级、乙肝表面抗原、血清AFP水平、肝硬化程度、有无转移、有无门静脉癌栓、肿瘤大体形态、肿块直径无明显相关(P>0.05)。C-myb蛋白在有肝内转移、有门静脉癌栓形成组中的阳性表达率及表达评分明显高于无肝内外转移、无门静脉癌栓形成组,差异有统计学意义(P<0.05)。而C-myb蛋白表达与年龄、性别、肝癌病理类型、病理分级、乙肝表面抗原、血清AFP水平、肝硬化程度、肿瘤大体形态、肿块直径无明显相关(P>0.05)。原发性肝癌组织中Glypican-3蛋白和C-myb蛋白的表达评分无明显关联(r=0.23,P>0.05)。结论1、Glypican-3蛋白在PHC癌组织中的高表达,而在癌旁肝脏组织中未见表达,表现出Glypican-3良好的敏感性和特异性,有望成为早期肝癌,尤其是AFP阴性的肝癌的诊断指标。2、C-myb蛋白在PHC癌组织中的表达明显高于癌旁肝脏组织,且其可能在PHC的侵袭转移中发挥了作用,可能是肝癌的不良预后因素,可能作为判断肝癌恶性程度的一个预测指标3、Glypican-3蛋白和C-myb蛋白在PHC组织中的表达不存在相关性,提示Glypican-3蛋白和C-myb蛋白在原发性肝癌的发生、发展中不存在明显关系。更多还原

【Abstract】 Objective To investigate the expression of Glypican-3 (GPC3) protein and proto-oncogene C-myb protein in Primary Hepatic Carcinoma (PHC), and to explore the relationships of themselves and the correlation between them.Methods The specimens from 50 patients with Primary Hepatic Carcinoma (PHC) and 15 corresponding paraneoplastic hepatic tissues were fixed in 10% formalin and routinely embedded in paraffin. The specimens were continuously sliced into 3μm-thick sections. The expressions of Glypican-3 and C-myb were detected by immunohistochemistry SP method and scored under high-power microscopy.Results There are significantly statistical distinctions in the expressive positive rate and the expressive scores of Glypican-3 and C-myb protein between cancer tissues and paraneoplastic tissues (P<0.01, P<0.05)We haven’t found the relatioships between Glypican-3 and main clinicopathological characteristics of PHC(P>0.05).There is no correlation between the expression of CAI protein and clinicopathological characteristics of PHC, except that the expressive positive rate and the expressive scores of PHC significantly higher with intrahepatic metatasis and tumor thrombosis than those which were without metastasis and tumor thrombosis.(P<0.05).There is no evident correlation between the expressive scores of Glypican-3 protein and that of C-myb protein in cancer tissues of PHC (r =0.23, P>0.05)Conclusion1. The high expression of Glypican-3 protein in PHC may play an impotant role in the diagnosis of PHC because of its sensitivity and especiallity.2. The expression of C-myb palys an important role in the invasive and metastatic part of PHC.It may be a harmful factor of prognosis.3. there is no correlation between the expressive scores of Glypican-3 protein and C-myb protein in cancer tissues of PHC更多还原