【作者】 梁正芬；

【导师】 王祝年；

【作者基本信息】 海南大学 ， 种质资源学， 2010， 硕士

【摘要】 本文分为两部分,第一部分概述了苦木科(Simaroubaceae)牛筋果属(Harrisonia)植物化学成分及药理活性研究进展。20世纪80年代初至今,前人已对牛筋果属(Harrisonia)植物中色原酮类、苦木素类、聚酮类、三萜类等有效化学成分及其抗肿瘤、抗疟、抗氧化和昆虫拒食活性等多种药理活性进行了研究。第二部分对苦木科(Simaroubaceae)牛筋果属Harrisonia)植物牛筋果[(Harrisonia perforata (Blanco) Merr.)]果实的乙醇提取物中的化学成分及其抗氧化活性和抗肿瘤活性进行了研究。1、在活性筛选指导下,从该植物果实的乙醇提取物中分离得到18个化合物,运用现代波谱分析技术鉴定了12个化合物：Ethyl 3,4,5-Trihdroxybenzoate(1)、4-ethylgallic acid(2)、Hydroxyhydroquinone(3)、Gallic acid(4)、β-Sitosterol(5)、β-Daucosterol(6)、Stigmasterol(7)、Protocatechuic acid(8)、Palmitic acid(9)、Linoleic acid(10)、Cis-Oleic acid(11)、5-hydroxymethyl-2-Furancarboxaldehyde(12);化合物1-12均为首次在该植物果实中分离得到。2、用DPPH法进行抗氧化活性测试,结果表明其醇提物部分、石油醚部分、乙酸乙酯部分具有较强的抗氧化活性；在活性追踪指导下,对单体化合物进行抗氧化活性测试,结果表明化合物1、2、3、4、8具有较强抗氧化活性。3、用MTT法对牛筋果果实乙醇提取物进行体外活性筛选,结果表明醇提物部分、石油醚部分、乙酸乙酯部分对人慢性髓原白血病细胞(K562)及人胃癌细胞(SGC-7901)均未表现出明显的生长抑制活性。4、采用气相色谱-质谱联用技术(GC-MS)对牛筋果果实脂溶性进行分析,确认了其果实石油醚部分29种成分(占总量的69.67%),其含量较高的成分依次为顺-9,12-十八碳二烯醇(12.35%)、棕榈酸(9.67%)、Z-9,17-十八碳烯醛(8.97%)、油酸乙酯(5.81%)、β谷甾醇(4.88%)、亚油酸乙酯(4.10%)；其乙醇粗提物的脂溶性成分共确认了其中的12种成分(占总量的71.00%),其中含量较高的成分依次为奎尼酸(40.44%)、焦桔酸(14.64%)、5-羟甲基-2-呋喃甲醛(5.89%)。更多还原

【Abstract】 The thesis consists of two parts. The first part is a review of the research progress of chemical constituents and their bioactives from the plants of the genus of Harrisonia R. Br.. There have been many researches about the fruits of [Harrisonia perforata (Blanco) Merr.] since 1980s. In recent several decades, chromones, triterpenoids, limonoids and prenylated polyketides with various bioactivities have been reported from H. Perforata.The second part is about the antioxidant and anti-tumor activity constituents from the ethanol extract of the fruits of H. Perforata.1、Bioassay-guided fractionation led to the isolation of 18 compounds from the ethanol extract of H. Perforata,12 compounds structures were unambiguously elucidated as Ethyl 3,4,5-Trihdroxybenzoate (1)、4-ethylgallic acid (2)、Hydroxyhydroquinone (3)、Gallic acid (4)、β-Sitosterol (5)、β-Daucosterol (6)、Stigmasterol (7)、Protocatechuic acid (8)、Palmitic acid (9)、Linoleic acid (10)、Cis-Oleic acid (11)、5-hydroxymethyl-2-Furancarboxaldehyde (12), and all of them were isolated from the fruits of the H. Perforata for the first time.2、With bioassay-guiding, the parts of Petroleum Ether extract, Ethyl Acetate extract and Ethanol extract possessed high antioxidant activity by DPPH free radical scavenging method; and compounds 1、2、3、4、8 showed high antioxidant activity respectively.3、In our screening for bioactive agents from tropical medicinal plants, the parts of Petroleum Ether extract, Ethyl Acetate extract and Ethanol extract of the fruits from H. Perforata did not show cytotoxic activity towards human chronic myelogenous leukemia (K562) and human gastric cancer (SGC-7901) cell lines in vitro by MTT method.4、This is the first report of gas chromatography-mass spectrometry of chemical constituents from the petroleum ether fraction and alcohol extraction of the fruits of H. Perforata, and the constituents were identified by their mass spectra. In the petroleum ether extraction,29 compounds were identified, amounting to 69.67% of the total detected constituents. The major constituents were identified as Z-9,12-Octadecadien-1-ol (12.35%), Palmitic acid (9.67%), Z-9,17-octadecadienal (8.97%), Ethyl Oleate (5.81%),β-Stitosterol (4.88%), Ethyl Linoleate (4.10%). From the alcohol extraction,12 compounds were identified, amounting to 71.00% of the total detected constituents. The major constituents were identified as Quinic acid (40.44%), pyrogallol (14.64%),5-(hydroxymethyl)-2-Furancarboxaldehyde (5.89%).更多还原