熟地杞子炖猪脑对AD小鼠学习记忆能力的影响

【作者】 史银飞；

【导师】 路新国；

【作者基本信息】 扬州大学 ， 营养与食品卫生学， 2010， 硕士

【摘要】 本研究旨在探讨熟地杞子炖猪脑在对老年性痴呆小鼠学习记忆能力及相关血清、脑组织生化指标的影响。选用4-5月龄清洁级的ICR健康小鼠112只,雌雄各半,按体重随机分为空白组、模型组、西药组(石杉碱甲)、熟地杞子炖猪脑高剂量组、熟地杞子炖猪脑中剂量组、熟地杞子炖猪脑低剂量组和淮山杞子炖猪脑组等七组。除空白组外,其余各组均采用东莨菪碱(SCOP)、Alcl3与D-gal三因素联合诱导,即实验开始的第1天起,每日于16:00以4mL/只Alcl3水溶液的量灌胃,同时按200mL/kg的剂量于腹腔注射D-gal注射液,并于实验开始的第5周起,中午12:00于腹腔注射2mL/kg的氢溴酸东莨菪碱注射液,建立AD模型。从实验开始第2天起,空白组与模型组每日7:30灌胃20mL/kg的蒸馏水,其余各组灌服相同剂量的药食,连续6周后检测各项指标。通过Y型迷宫测试小鼠的学习记忆能力的变化,用化学比色法测定小鼠血清及脑组织内丙二醛(MDA)的含量和脑组织内乙酰胆碱酯酶(AChE)的活力,用黄嘌呤氧化酶法测定小鼠血清及脑组织内超氧化物歧化酶(SOD)的活力,运用全自动生化分析仪测定血清胆碱酯酶(ChE)活力和血清总胆固醇的含量,并用光学显微镜观察小鼠脑海马组织形态结构的变化。采用SPSS18.0统计软件对数据进行统计分析显示:与模型组相比,空白组和熟地杞子炖猪脑中剂量组小鼠在体重、学习记忆能力和其他生化指标上均达到显著性差异(P<0.01)。西药组在血清ChE活力和脑组织AChE活力上优于其他药食组,熟地杞子炖猪脑中剂量组在体重、学习记忆能力和血清及脑组织内MDA的含量及超氧化物歧化酶(SOD)的活力等方面,效果较好。在光镜下,小鼠海马CA3区的切片图中模型组小鼠变化最为明显,熟地杞子炖猪脑中剂量组与空白组的变化差异性不显著。根据结果可得出以下结论:东莨菪碱、Alcl3与D-gal三因素联合诱导对4-5月龄的ICR小鼠的AD模型建造是成功的。熟地杞子炖猪脑能改善AD模型小鼠的学习记忆障碍。熟地杞子炖猪脑对AD模型小鼠海马组织病理改变具有改善作用。熟地杞子炖猪脑可增强SOD的活性,减少MDA在体内的含量,从而改善因氧化引起的氧自由基的改变,发挥其抗氧化作用。熟地杞子炖猪脑能抑制AChE或ChE的活性,降低乙酰胆碱的水解速度起到防治AD的作用。更多还原

【Abstract】 In this paper, the author’the purpose of this research is to discuss the effect of Shudi Gouqi Dun Zhunao to the senile dementia mouse in learning and memory ability and the related blood serum, the brain organization biochemistry target influence. Selects April to May age clean level ICR healthy mouse 112, is half and half female and male, divides into 7 groups according to the body weight : blank group, model group, western medicine group, Shudi Gouqi Dun zhunao high dose group, Shudi Gouqi Dun zhunao middle dose group, Shudi Gouqi Dun zhunao low dose group and Huaishan Qizi Dun Zhunao. All groups except the blank group, uses the scopolamine (SCOP), Alcl3 and the D-gal three factor union induction, namely the 1st day starts 16:00 to fill stomach 4mL/d in the afternoon the Alcl3 peroxide solution only and injects 200mL/kg D-gal, and starts in the 5th week, noon 12:00 injects 2mL/kg the scopolamina hydrobromidum inoculation fluid, establishes the AD model. The 2nd day starts 7:30 to fill stomach 20mL/kg every day in the morning the distilled water, other each group fills the clothing commensurability the medicine food and examines each target after 6 weeks. Through the Y-maze test of learning and memory ability in mice changes in chemical colorimetric determination of serum and brain tissue malondialdehyde (MDA) content and brain tissue acetylcholinesterase (AChE) of energy, with the yellow Determination of purine oxidase superoxide dismutase (SOD) of the activity, using automatic biochemical analyzer determination of serum cholinesterase (ChE) activity and serum total cholesterol levels, and with an optical microscope hippocampus morphology in mice changes.SPSS18.0 statistical software used for statistical analysis of data and found that Compared with model group, control group and Shudi Gouqi Dun zhunao middle dose groups in body weight, learning and memory abilities, and other biochemical indicators were significant differences ( P <0.01). Western medicine group in serum ChE activity and brain AChE activity than other drugs groups, Shudi Gouqi Dun zhunao middle dose in body weight, learning and memory ability and brain tissue and serum MDA content and superoxide dismutase ( SOD).The viability so that the effect is good. In the light microscope, hippocampal CA3 area of slices in the model group were the most obvious change, Shudi Gouqi Dun zhunao middle dose group and control group did not change significantly.The following conclusions can be drawn based on the results: scopolamine, Alcl3 with the D-gal induced by three factors, the AD model mice on the ICR to build is a success. Shudi Gouqi Dun zhunao can improve the AD model of porcine brain stew of learning and memory impairment in mice. Shudi Gouqi Dun zhunao AD stew Porcine Model of pathological changes in hippocampus of mice with the improvement. Shudi Gouqi Dun zhunao SOD stew porcine brain activity can be enhanced to reduce the MDA content in the body, thereby improving the oxidation caused by oxygen free radicals due to the change to play its antioxidant activity. Shudi Gouqi Dun zhunao can inhibit AChE or stew porcine brain ChE activity, reduce the rate of hydrolysis of acetylcholine and thus increase its concentration in the patient’s body and thus play a role in AD prevention and treatment.更多还原