【作者】 曾何华；

【导师】 吐尔洪·买买提；

【作者基本信息】 新疆大学 ， 化学， 2010， 硕士

【摘要】 本论文在详细细综述分子印迹技术和固相萃取的研究现状和实际样品中齐多夫定、拉米夫定分析方法最新进展的基础上,通过非共价本体聚合法分别合成了以齐多夫定和拉米夫定为模板的分子印迹聚合物,将所得的材料作为固相吸附剂,探讨了在实际样品中选择性固相萃取齐多夫定和拉米夫定等方面的应用。主要内容包括:1、将计算机模拟与渗漏实验相结合,筛选出最佳功能单体;合成拉米夫定的替代模板拉米夫定酯化产物;通过紫外光谱法研究模板分子齐多夫定与功能单体甲基丙烯酸及拉米夫定酯化产物与功能单体甲基丙烯酸形成主客体复合物的自组装过程,此方法为制备抗HIV药物分子印迹聚合物及识别聚合机理提供了依据。2、合成以甲基丙烯酸为功能单体,三甲氧基丙基三甲基丙烯酸酯为交联剂的齐多夫定分子印迹聚合物;以及以甲基丙烯酸为单体、乙二醇二甲基丙烯酸酯为交联剂的拉米夫定替代模板-拉米夫定酯化产物分子印迹聚合物。将其用于固相萃取,通过对上样、除杂、洗脱等条件进行优化,成功的将齐多夫定和拉米夫定从其结构类似物的混合物中分离出来,为实际样品的分离与富集奠定基础。同时虚拟模板技术可以很好的解决聚合物应用中“模板渗漏”的问题。3、将分子印迹技术与固相萃取联用,成功的去除了药片及血清中的干扰组分,净化了样品,并通过高效液相色谱法测定了血清中抗HIV药物的含量:齐多夫定不同加入量的回收率73.8%~76.3%;拉米夫定不同加入量的回收率为69.9%~75.0%。该方法直观、快速,可用于其它生物体液中药物的分离与富集。更多还原

【Abstract】 In this research, molecular imprinting technique and solid-phase extraction were reviewed in detail. In addition the advances were in analytical methods of zidovudine (AZT) and lamivudine(3TC) in real samples were summarized. Using non-covalent bulk polymerization method, AZT molecularly imprinted polymers (MIP) and 3TC MIP were synthesized and packed into the SPE cartridges. The application of molecular imprinting solid-phase extraction(MI-SPE) in real samples was discussed. The main results of the studies are as follows:1. The best monomer was chosen by combining computer simulation and breakthrough experiment; the esterified product of 3TC(3TC-Es) was synthesized. Besides, Molecular imprinting was used to develop a method based on non-covalent interaction for the synthesis of AZT and 3TC-Es imprinted polymer. The molecular recognition characteristics of the imprinting polymers were evaluated by UV. This method provided basic evidence for synthesizing anti-HIV drugs molecularly imprinted polymers and analysising its mechanism.2. Using methacrylic acid as functional monomer, and trimethlolpropane trimethacrylate as cross linking agent, MIP specific for AZT was synthesized; in the same way, using methacrylic acid as functional monomer, ethylene glycoldi methacrylate as cross linking agent, a MIP specific for 3TC was synthesized using 3TC analog 3TC-Es as template, then were packed into SPE cartridges. By optimizing the loading, washing and eluting conditions, AZT, 3TC and their analogues were separated by the MI-SPE method successfully. It laid foundation for separation and enrichment of drugs in real samples. At the same time, the problem of“template leakage”was solved successfully by suppositional molecular imprinting technique.3. After cleaning off the interfering compounds, the content of anti-HIV in real samples was determined: It was found that MI-SPE-HPLC can be used for the determination of AZT and 3TC. The recoveries of AZT in serum were in the range of 73.8%~76.3%, and 3TC in serum were 69.9%~75.0%. This method is rapid, intuitive, also can be used to separate and enrich drugs of other biomedical sample.更多还原