【作者】 韩白；

【导师】 冯嵬；

【作者基本信息】 北京化工大学 ， 化学工程与技术， 2010， 硕士

【摘要】 碳纳米管在生物和医药方面有着潜在的应用价值。但是碳纳米管不能在任何溶剂中分散,并且具备一定的细胞毒性,这些缺点严重制约了纳米管的应用。本论文使用正十六胺和D-葡萄糖醛酸-γ-内酯合成了一种新的物质：N-十六烷基-D-葡糖酰胺,可以有效的分散碳纳米管,并具有良好的生物相容性。本论文使用所合成的N-十六烷基-D-葡糖酰胺在不同浓度的甲醇、乙醇、异丙醇的水溶液以及纯水中吸附碳纳米管形成纳米复合体。发现在一定比例的醇-水混合液中形成的纳米复合体分散性优于在水中形成的复合体。复合体在水中重新分散后,分散性也显著增加。分散效果最好的是在体积分数为20%的甲醇-水溶液中形成的复合体。经过检测,发现糖基表面活性剂是通过物理作用吸附在碳纳米管上并对纳米管进行分散的,这种吸附作用没有被破坏碳纳米管的结构。实验使用制备的纳米复合体按照碳纳米管：牛血清白蛋白质量比为1：1、1：0.8、1：0.6三个比例吸附BSA。在25℃、45℃、65℃三个温度下测定不同溶液中形成的纳米复合体吸附牛血清白蛋白后对牛血清白蛋白二级结构的影响。结果发现,该纳米复合体吸附牛血清白蛋白后,对牛血清白蛋白的二级结构影响不大,其α螺旋均保持在74%以上。并且碳纳米管吸附糖基表面活性剂越多,复合体的分散性就越好,所吸附蛋白的结构保持率就会越高。在20%甲醇中制备的纳米复合体所吸附的牛血清白蛋白二级结构保持率高达85%。研究发现被吸附蛋白对温度的敏感性与游离蛋白一致,45℃和65℃条件下,蛋白二级结构中Q螺旋的保持率分别在90%和70%左右。更多还原

【Abstract】 Carbon nanotubes(CNTs) has potential applications on biology and medicines. But CNTs can not disperse in all the solvents and it has toxicity for cells. These disadvantages limit its applications.This paper synthesised a new type of sugar-based surfactant. It can increase CNTs’disperse ability and decrease the toxicity of CNTs.The CNTs sorption N-Hexadecyl-D-gluconamide in different concentration of methanol, ethanol and isopropanol can disperse better than sorption in water. The compound has greatest disperse ability is which was treated in 20% methanol. The N-Hexadecyl-D-gluconamide sorption on CNTs via physics adsorption effect and does not change the structure and character of CNTs.The compound adsorb Bovine Serum Albumin(BSA) by the mass ratio of 1:1,1:0.8 and 1:0.6 respectively. The resulting solution were assayed at different temperatures of 25℃,45℃, 65℃to assay the change of protein’s secondary structure after be adsorbed. The results indicate that after be adsorbed, the change of secondary structure is tiny. The retainα-helix amplitude is more than 74%. Nanocomposites preparation in 20% methanol keep the secondary structure best,the retainα-helix can even more than 85%. More sugar-based surfactant be adsorbed by CNTs, the disperse ability will be better, the secondary structure of BSA will be keep more perfectly. The research indicates that the sensibility to temperature of BSA does not changed before and after be adsorbed. At the temperature of 45℃and 65℃, the retainα-helix are about 90% and 70%both free BSA and the adsorbed BSA.更多还原