【作者】 李宗臻；

【导师】 冯嵬；

【作者基本信息】 北京化工大学 ， 化学工程与技术， 2010， 硕士

【摘要】 碳纳米管是一种重要的载体材料,在生物医学领域具有独特的应用价值,但是由于其在任何溶剂中都不溶也不能稳定分散的特性,使得它在这个领域的应用出现了障碍。在众多的改性碳纳米管的方法中,本文采用了用两性小分子的表面活性剂来非共价修饰碳纳米管,提高它在水中的分散性,从而可以为下一步的应用打下基础。本论文对两性小分子表面活性剂在有机相中的制备条件等进行了一定的探索,并成功制得了可以分散碳纳米管的两性小分子。利用市售的葡萄糖酸内酯和常见的化学试剂(纯度>99%)——过量的苄胺液体(分析纯),在无水甲醇中进行反应。本文中所用的碳纳米管为CVD法生成的多壁碳纳米管。实验室采用一定浓度的硝酸溶液进行氧化纯化多壁碳纳米管,将碳纳米管表面的这些多余的杂质氧化去除,剩余纯化的碳纳米管。本论文采用甲醇、乙醇、异丙醇等不同浓度的水溶液以及纯水作为表面活性剂分散碳纳米管的初始处理溶剂,并对处理后的碳纳米管分散水溶液进行紫外-可见光谱全波长扫描,最终确定了异丙醇水溶液为碳纳米管的最佳处理溶剂以及最佳浓度30%,甲醇、乙醇水溶液的最佳浓度分别为15%,15%,这就奠定了下一步的研究基础。用经过各个最佳浓度的溶剂处理之后的碳纳米管,对实验中的牛血清蛋白分子进行有效的吸附,并通过紫外-可见分光光度计全波长扫描,以及在三种不同的温度(25℃、45℃、65℃)下通过圆二色光谱仪考察牛血清蛋白被分散性的碳纳米管吸附之后二级结构的变化情况,并利用一定的软件对蛋白二级结构中的主要成分进行计算,定量的分析碳纳米管吸附蛋白的性能以及其对蛋白二级结构的影响。更多还原

【Abstract】 Carbon nanotube is an important material as carrier which has particular applied value in the field of Biological and medical science.while, their characteristic nature of insoluble in any solvent, largely limits their application.Among so many modified methods of carbon nanotubes, we choose small amphiprotic molecule to modify carbon nanotubes by non covalent bond, so the disperstiveness of the modified carbon nanotubes in water will be increased.In this paper, we have quested the Preparation Conditions of the small amphiprotic molecule which can be a well surface active agent to carbon nanotubes. The glucolactone we used in the laboratory was available in the market, and its purity quotient is>99%,and another reactant is superfluous benzylamine (analytically pure). The reaction happened in absolute methanol under determinate condition.The carbon nanotubes we used in this paper is prepared by the method of CVD. It was purified by the aqueous solution of nitric acid which is a certain consistency. In this process, the excrescent impurityies at surface of carbon nanotubes were removed by the way of oxygenize, and the pure carbon nanotubes were received.In this paper, we used different consistency of methanol, ethanol, and isopropanol aqueous solution as the initial processed solvents of carbon nanotubes. And the processed carbon nanotubes dispersed again in the water to obtain a well dispersion, which were detectd by the ultraviolet spectrograph. So we can choose out the best initial processed solvents of carbon nanotubes. They are 15% methano,15% ethanol,30% isopropanol, in which the 30%isopropanol is the best one.The carbon nanotubes processed by the best initial processed solvents can adsorb BSA(Bovine Serum Albumin) in water. And the solutions were detected by ultraviolet spectrograph and the Circular Dichroism Spectrometer at three different temperatures(25℃、45℃、65℃). In addition to this, the changes of secondary structure in BSA (Bovine Serum Albumin) can be calculated by a soft named CDpro. So we can analyze the structure of BSA in ration.更多还原