【作者】 贾华；

【导师】 赵斌；

【作者基本信息】 河北师范大学 ， 运动人体科学， 2010， 硕士

【摘要】 随着全球老年人口的增多,世界各国出现不同程度的老龄化问题,衰老问题逐渐受到各国政府和社会的关注。衰老进程中,心脏早于其他器官,出现结构和功能的退行性变化,严重影响老年人的健康,而自由基产生与清除的动态平衡打破,被认为是心肌衰老的重要原因之一。延缓心肌衰老的干预方式诸多,其中以运动、饮食的效果最佳。本研究对大鼠注射D-半乳糖复制亚急性衰老模型,在6周造模过程中,同时施加负重训练和补充大豆多肽干预,观察衰老大鼠血清和心肌匀浆指标的变化,并分析其对衰老大鼠心肌抗氧化能力的作用效果,为不久的人群实验积累一定的理论基础和实验参考,同时也为在中老年人群中推广有效的运动方式、合理有效营养补剂以及运动与营养相结合的新方法提供理论依据和实践指导。本研究以3月龄SD雄性健康大鼠56只为研究对象,随机分为7组:成年对照组(C)、D-半乳糖模型组(M)、D-半乳糖小负重组(S)、D-半乳糖大负重组(B)、D-半乳糖补肽组(P)、D-半乳糖补肽小负重组(SP)和D-半乳糖补肽大负重组(BP)。除C组,其他各组在6周造模过程中,分别进行跑台负重训练和补充大豆多肽干预。6周末处死,比较各组血清和心肌匀浆生化指标。结果1. 6周末,与C组相比,M组大鼠血清MDA含量显著性升高(P<0.05)、SOD活性非常显著性下降(P<0.01)。2. 6周末,与C组相比,M组大鼠心肌组织MDA、脂褐素含量非常显著性升高(P<0.01,P<0.01)。负重训练能够使衰老大鼠心肌组织MDA、脂褐素含量显著性下降(P<0.05,P<0.05);补充大豆多肽能够使衰老大鼠心肌组织MDA、脂褐素含量出现下降趋势(P>0.05,P>0.05),两种干预手段具有显著性或非常显著性交互作用(P<0.05,P<0.01)。3. 6周末,与C组相比,M组大鼠心肌组织SOD、CAT、GSH-PX活性非常显著性下降(P<0.01,P<0.01,P<0.01)。负重训练或补充大豆多肽均能够使衰老大鼠心肌组织SOD、GSH-PX活性显著性升高(P<0.05,P<0.05),两种干预手段具均有显著性交互作用(P<0.05,P<0.05)。而负重训练或补充大豆多肽均能够使衰老大鼠心肌组织CAT活性出现升高趋势(P>0.05,P>0.05),两种干预方式具有显著性交互作用(P<0.05)。4. 6周末,与C组相比,M组大鼠心肌组织NO含量非常显著性下降(P<0.01)。负重训练或补充大豆多肽均能使衰老大鼠心肌组织NO含量出现升高趋势(P>0.05),两种干预方式具有显著性交互作用(P<0.05)。5. 6周末,与C组相比,M组大鼠心肌组织Na+-K+-ATPase和Ca2+-Mg2+-ATPase活性非常显著性下降(P<0.01, P<0.01)。负重训练能够使衰老大鼠心肌组织Na+-K+-ATPase活性显著性升高(P<0.05),而补充大豆多肽能够使衰老大鼠心肌组织Na+-K+-ATPase活性出现升高趋势(P>0.05),两种干预手段具有显著性交互作用(P<0.05)。负重训练或补充大豆多肽能够使衰老大鼠心肌组织Ca2+-Mg2+-ATPase活性非常显著性或显著性升高(P<0.01, P<0.05),两种干预方式具有显著性交互作用(P<0.05)。结论1.通过6周D-半乳糖皮下注射,模型组大鼠血清MDA含量升高、SOD活性降低,心肌组织脂褐素含量升高,以及外观衰老症状出现和行为的变化,说明本实验衰老造模成功。2. 6周负重训练或补充大豆多肽均能够不同程度的提高衰老大鼠心肌抗氧化酶活性,减少脂质过氧化产物的生成;负重训练结合补充大豆多肽,两者交互作用显著,共同进一步增强衰老大鼠心肌抗氧化能力,其中以小负重补肽组效果最佳,而且抗氧化方面表现的比较全面,升高心肌组织SOD、CAT、GSH-PX活性和NO含量,降低MDA和脂褐素含量。3. 6周负重训练或补充大豆多肽均能够不同程度提高衰老大鼠心肌组织Na+-K+-ATPase和Ca2+-Mg2+-ATPase;负重训练结合补充大豆多肽,两者交互作用显著,进一步增强衰老大鼠心肌功能酶活性,减少自由基对细胞生物膜的破坏,维持细胞生物膜的稳定性,增强离子的转运,调节能量代谢,延缓心肌衰老。更多还原

【Abstract】 With the increasing of the elderly population in the world, there are different degree aging problems, which gradually become an obvious attention in many countries and society. In aging process, heart is earlier than other organs, which appears degenerative changes in the structure and function, seriously affecting the health of the elderly, while the dynamic balance of production and scavenging of free radicals to break, is considered one of the important reasons of myocardial aging. There are many interventions delaying myocardial aging, and sport, diet is best. In this study, rats were injected D-galactose to copy subacute aging model, imposing weight training and soybean peptides supplement in 6 weeks modeling process. We observed changes of serum and myocardial indicators in aging rats and analyze the effects of myocardium antioxidant capacity, in order to accumulate a certain theoretical basis and experimental reference for the future crowd experiments, but also promote effective sports pattern, rational nutritional supplement and the new method of combining sport with nutrition to provide theoretical basis and practical guidance in the elderly population.In this study, 56 three-month-old SD male healthy rats were randomly divided into 7 groups: control group (C), model group (M), small load exercise group (S), big load exercise group (B), peptide group (P), small load exercise and peptide group (SP), big load exercise and peptide group (BP). Except group C, the other groups were interfered respectively treadmill weight training and the soybean peptides in 6 weeks modeling process. After 6 weeks, all groups were killed to compare the serum and myocardial biochemical markers.Results1. After 6 weeks, compared with group C, the serum MDA level was higher significantly (P<0.05), and SOD vitality was very lower significantly in group M (P<0.01).2. After 6 weeks, compared with group C, myocardium MDA and lipofuscin level were very lower significantly in group M (P<0.01, P<0.01). Weight training made myocardium MDA and lipofuscin level of aging rat decrease significantly (P<0.05, P<0.05); Soybean peptides supplement made myocardium MDA and lipofuscin level of aging rat have declining trend (P>0.05, P>0.05), and they had significant or very significant interaction (P<0.05, P<0.01).3. After 6 weeks, compared with group C, myocardium SOD, CAT, GSH-PX vitality were very lower significantly in group M (P<0.01, P<0.01, P<0.01). Weight training or soybean peptides supplement made myocardium SOD, GSH-PX vitality of aging rat decrease significantly (P<0.05, P<0.05), and they had significant interaction (P<0.05, P<0.05). Weight training or soybean peptides supplement made myocardium CAT vitality of aging rat have increased trend (P>0.05, P>0.05), but they had significant interaction (P<0.05).4. After 6 weeks, compared with group C, myocardium NO level was very lower significantly in group M (P<0.01). Weight training or soybean peptides supplement made myocardium NO level of aging rat have increased trend (P>0.05), but they had significant interaction (P<0.05).5. After 6 weeks, compared with group C, myocardium Na+-K+-ATPase and Ca2+-Mg2+-ATPase vitality were very lower significantly in group M (P<0.01, P<0.01). Weight training made myocardium Na+-K+-ATPase vitality of aging rat increase significantly (P<0.05), and soybean peptides supplement made myocardium Na+-K+-ATPase vitality of aging rat have increased trend (P>0.05), but they had significant interaction (P<0.05). Weight training or soybean peptides supplement made myocardium Ca2+-Mg2+-ATPase vitality of aging rat very increase significantly or increase significantly (P<0.01, P<0.05), and they had significant interaction (P<0.05)Conclusions1. Through the 6 weeks D-galactose subcutaneous injection, the serum MDA level increased and SOD vitality decreased , and myocardium lipofuscin level increased, as well as the appearance of aging symptoms and behavioral changes in group M. These indicated the rat aging model can be copied successfully.2. 6 weeks weight training or soybean peptides could enhance the different levels of myocardial antioxidant enzymes in aging rat, and reduce the generation of lipid peroxidation. Combination weight training with soybean peptides had notable significant interaction to enhance myocardial antioxidant capacity of aging rat, in which small load exercise and peptide group was best. And antioxidant appear comprehensive performance: myocardium SOD, CAT, GSH-PX vitality and NO level increased , and MDA and lipofuscin level decreased.3. 6 weeks weight training or soybean peptide supplement could improve myocardium Na+-K+-ATPase and Ca2+-Mg2+-ATPase vitality of aging rat. Combination weight training with soybean peptides had notable significant interaction to enhance myocardial Na+-K+-ATPase and Ca2+-Mg2+-ATPase vitality, reduced free radical damage to cell membranes, maintain cell membrane stability, enhanced ion transport,regulated energy metabolism and delayed myocardium aging.更多还原