【作者】 武俊香；

【导师】 贾因棠；

【作者基本信息】 山西医科大学 ， 内科学， 2010， 硕士

【摘要】 目的：功能性DNA多态性影响基因表达以及血浆或组织中IL-10的水平。我们的病例对照研究主要是要探索白细胞介素-10(IL-10)基因启动子区域-1082,-592位点单核苷酸多态性(SNP)与乙型肝炎病毒感染后临床转归之间的关系.方法：我们采用病例-对照研究的方法,分别收集175例乙肝患者和153例正常对照者的外周静脉血,以基因组DNA为模板,采用聚合酶链反应-限制性片段长度多态性方法(PCR-RFLP)分析IL-10-592A/C位点和IL-10-1082A/G位点基因分布及等位基因频率,并结合临床资料初步评价IL-10-592A/C位点和IL-10-1082A/G点基因多态性与乙型肝炎病毒感染后的相关性研究。用x2检验进行统计学分析。结果：①中国山西地区汉族人群中IL-10-592A/C基因多态性存在AA、AC和CC三种基因型,但这三种基因型的分布在患者组和对照组无统计学差异,且在患者组各种临床分型的基因型分布比较中亦无统计学差异。②IL-10-1082位点在正常对照组有AA,AG,GG三种基因型,而患者组只发现AA,AG两种基因型。经统计学分析,在基因型分布上,患者组和对照组无统计学差异。患者组各临床分型组进行比较,慢性乙型肝炎组与急性乙型肝炎组,重症肝炎组比较均有统计学差异(P值<0.001,<0.05)；而与肝硬化组却无明显统计学差异。另外,肝硬化组与急性乙型肝炎组基因型分布亦有统计学差异(P=0.020)。结论：IL-10-592A/C位点和IL-10-1082A/G位点的SNP与乙型病毒性肝炎易感性之间并无关联性。而IL-10-1082A等位基因及AA基因型与乙肝病毒感染及感染后转归相关。更多还原

【Abstract】 Objective To investigate the correlation between the single nuclertide polymorphism in the promoter region of interleukin-10(IL-10) gene and the clinical phenotypes of hepatitis B virus(HBV)infection in Chinese population。Methods A retrospective case-control study design was applied for the present study. Using genomic DNA as templates, The genotypes of-1082 and-592 loci in the promoter region of IL-10 were analysis by polymerase chain-restriction fragment length polymorphism method (PCR-RFLP) in 175 cases of patients with hepatitis B and 153 cases of the control.And Preliminary evaluate the association between the genotypes of-1082 and-592 loci in the promoter region of interleukin-10 polymorphisms with hepatitis B virus infection consequence.Chi-square test (χ2 test) was used for statistical.Results Genotype AA,AC,CC in loci-592 in the promoter region of IL-10 were found in both patients group and the control group from Chinese Han population of Shanxi, and the genotypes distribution in two groups has no significant difference (P>0.05). The-1082AA and-1082AG genotype in IL-10 promoter region were only found in the group of patients with hepatitis B, but-1082 A A,-1082AG and-1082GG genotypes were all found in the control group. the genotypes distribution in two groups has no significant difference.But the distribution frequences of-1082A allele and-1082AA genotype in IL-10 promoter region were significantly higher in the group of patients with chronic hepatitis B than the group of patients with acute hepatitis B and the group of patients with sever hepatitis B (P 0.001,0.05)。Morever,the distribution between the group of Cirrhosis and the group of acute hepatitis B has significant difference(p=0.020).Conclusion 1.IL-10-592A/C and IL-10-1082A/G Single nucleotide polymorphism with the occurrence of hepatitis B has no significant difference.2.The-1082A allele and -1082AA genotype in IL-10 promoter region may be associated with the chronic hepatitis B infection.更多还原