【作者】 王会龙；

【导师】 张莉芸；

【作者基本信息】 山西医科大学 ， 内科学， 2010， 硕士

【摘要】 目的：通过检测雷公藤多甙(GTW)治疗AS患者不同时间点血清中基质金属蛋白酶(matrix metalloproteinase, MMP)-3和白介素-6 (interleukin, IL-6)的表达水平变化,分析其与AS疾病活动的相关性,并探讨GTW治疗AS发挥作用的可能机制。方法：1.分别在治疗0、2、6、12周时留取本研究临床研究中22例AS患者空腹血清,应用酶联免疫吸附试验(ELISA)测定MMP-3和IL-6水平。2.分析血清MMP-3、IL-6表达水平与AS疾病活动指标如强直性脊柱炎疾病活动指数(BASDAI)、强直性脊柱炎功能指数(BASFI)、血沉(ESR)和C反应蛋白(CRP)等之间的相关性。结果：ELISA方法检测22例AS患者GTW治疗不同点血清MMP-3、IL-6水平,结果显示：1.12周时,血清MMP-3水平与O周有显著下降(P<0.01),0、12周时与BASDAI、BASFI、ESR (P<0.05)相关,而与病人总体评估(PGA)、夜间痛、脊柱痛、脊柱炎症、强直性脊柱炎测量指数(BASMI)、肌腱端指数(EI)、关节肿胀数和扩胸度无相关性。2.6周、12周时,血清IL-6水平与0周有显著下降(P<0.01),而二者间无统计学意义(P>0.05)；0周、6周时IL-6水平与脊柱炎症及ESR显著相关(P<0.01),12周时IL-6水平与BASDAI显著相关(P<0.01)。结论：1.GTW能降低患者血清MMP-3和IL-6水平,这可能是其发挥临床疗效的机制。2.血清MMP-3和IL-6可作为预测GTW治疗AS疾病进展及病情活动的潜在生物学标志物。更多还原

【Abstract】 ObjectiveTo investigate the changes of serum matrix metalloproteinase(MMP)-3 and interleukin(IL-6 level in different times treating ankylosing spondylitis(AS) with multi-glycosideofTripterygium wilfordii Hook. f. (GTW) and to explore their meaning in diseases activity and possible link to therapy response.MethodsPeripheral bloods of 0,2,6,12 weeks were collected from 22 patients with AS who received GTW. Serum MMP-3 level and IL-6 level were measured by sandwich enzyme-linked immunosorbent assay(ELISA).Further respectively analyze the relationship among MMP-3, IL-6, ankylosing spondylitis disease activity index (BASDAI), ankylosing spondylitis functional index (BASFI), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and so on.Results1. The MMP-3 level decreased statistically after 12 weeks GTW treatment with 0 week(P<0.01). The level of MMP-3 showed correlation with BASDAI, BASFI, ESR at 0 week or 12 weeks and no correlation with other indexes.2. The IL-6 level decreased statistically at 6 weeks or 12 weeks after GTW treatment compared with 0 week(P<0.01), but no variability between them. The level of IL-6 showed correlation with spinal inflammation and ESR at 0 week or 12 weeks and with BASDAI at 12 weeks, and no correlation with other indexes.Conclusion1. The therapy of GTW can effectively cut down the serum MMP-3 level and IL-6 level in AS patients. This is likely to be a relevant mechanism for the clinical efficacy of GTW therapy.2. Serum MMP-3 and IL-6 level could be used as potential biomarkers to evaluate disease progression and disease activity.in AS patients of GTW treatment..更多还原