【作者】 张洪志；

【导师】 周士福；

【作者基本信息】 苏州大学 ， 外科学， 2010， 硕士

【摘要】 目的:检测水通道蛋白5(AQP5)在大肠癌组织中的表达情况,分析其在大肠癌发生、发展及转移过程中的临床意义。方法:选择2006年11月至2007年12月手术切除后并经病理证实的大肠癌患者30例,采用逆转录聚合酶链反应(RT-PCR),免疫印迹(Western-blot),免疫组织化学的方法检测AQP5在人大肠癌组织和癌旁正常组织中的表达情况。结果:RT-PCR:在大肠癌组织中均有AQP5 mRNA的表达,AQP5/GAPDH比值为0.42±0.08,而在癌旁正常组织中几乎没有AQP5 mRNA的表达,AQP5/GAPDH比值为0.02±0.00。AQP5与GAPDH灰度值相比后统计分析证实,AQP5 mRNA在大肠癌组织中表达较癌旁正常组织表达明显增多(P<0.05),内参GAPDH的PCR产物在各组中表达无显著性差异(P>0.05)。Western-blot:大肠癌组织标本均有AQP5蛋白表达,AQP5蛋白条带位置为35kD。半定量比较各种组织AQP5蛋白水平,大肠癌组织AQP5/GAPDH为0.74±0.12,癌旁正常组织AQP5/GAPDH为0.01±0.00,蛋白条带灰度值分析表明:大肠癌组织中AQP5蛋白表达量较癌旁正常组织明显增多(P<0.05),内参GAPDH蛋白表达在各组无显著性差异( P > 0.05)。免疫组织化学:AQP5阳性反应呈棕黄色,主要存在于大肠癌组织中肿瘤细胞的细胞膜及细胞浆,而在癌旁正常组织几乎没有表达。AQP5蛋白表达程度与肿瘤分化程度、肿瘤浸润深度、淋巴结转移及TNM分期相关(P<0.05)。结论:在大肠癌组织中AQP5的高表达,提示整个AQP5在人大肠癌的发生、发展及转移的过程中可能起重要作用。AQP5蛋白表达程度与肿瘤分化程度、肿瘤浸润深度、淋巴结转移及TNM分期相关,或许可以通过AQP5作为干预靶点来抑制大肠癌的生长和转移,为肿瘤的治疗提供一个新的途径。更多还原

【Abstract】 Objective: To investigate expression of aquaporin5 (AQP5) in human colorectal carcinoma tissues and analyze its clinical significance.Method: Human colorectal carcinoma tissues were acquired immediately after operation from November 2006 to December 2007. The expression of aquaporin5 in human colorectal carcinoma tissue and paraneoplastic normal tissue was detected by reverse transcription PCR (RT-PCR), Western-blot, Immunohistochemistry.Results: Findings by RT-PCR indicated that the AQP 5 mRNA expressed in colorectal carcinoma tissues was significantly higher than that expressed in paraneoplastic normal tissues (carcinoma tissues vs. normal tissues: 0.42±0.08 vs. 0.02±0.00, P<0.05). Weston blot showed that the AQP5 protein band was located at 35 kD. Furthermore, the AQP5 protein was significantly higher expressed in colorectal carcinoma tissues compared with paraneoplastic normal tissues (carcinoma tissues vs. normal tissues: 0.74±0.12 vs. 0.01±0.00, P<0.05). Immunohistochemistry showed that the AQP5 was mainly expressed in colorectal carcinoma cell membrane and cytoplasm. However, no AQP5 was detected in paraneoplastic normal tissues. The expression of AQP5 was related with the loss of tumor differentiation, lymph node metastasis and TNM stage (P<0.05).Conclusion: The overexpression of AQP5 in human colorectal carcinoma tissue plays an important role in colorectal carcinogenesis, progression and metastasis; The expression of AQP5 was related with the loss of tumor differentiation, lymph node metastasis and TNM stage. Furthermore, the AQP5 inhibtors may be regarded as novel targets of anti-cancer therapy.更多还原