【作者】 罗春玉；

【导师】 唐立；

【作者基本信息】 大连医科大学 ， 生物化学与分子生物学， 2010， 硕士

【摘要】 目的：研究赤峰地区蒙古族溃疡性结肠炎患者活动期和缓解期肠道优势菌群的变化,探讨UC患者肠道优势菌群的变化与疾病发生、发展的关系,为临床治疗UC提供依据。方法：病例采集及菌群分析法1.收集2008年10月-2009年12月我地区两家三级医院消化内科收治的蒙古族溃疡性结肠炎确诊患者32例,根据Sourtherland疾病活动指数(DAI),将病例分为UC活动期和UC缓解期两组。2.选取来我院正常体检的蒙古族人员18例作为正常对照组。3.采集病例组与对照组人员粪便进行肠杆菌、肠球菌、葡萄球菌、酵母菌、类杆菌、双歧杆菌、消化球菌、乳酸杆菌、优杆菌和梭菌10种肠道优势菌群定量分析。结果：1.与正常对照组比较,活动期溃疡性结肠炎患者肠道菌群中肠杆菌(9.00±0.19,p<0.01)、肠球菌(8.04±0.38,p<0.01)的数量明显增加,梭杆菌(6.83±0.34,p<0.05)的数量略有增加;双歧杆菌(7.34±0.29,p<0.01)的数量明显下降,其他细菌无明显变化。2.缓解期溃疡性结肠炎患者肠道菌群中肠杆菌(8.54±0.24,P<0.05)仍呈增加趋势,双歧杆菌、优杆菌数量略有下降,但无显著性差异,其他细菌无明显变化。结论：1.赤峰地区蒙古族溃疡性结肠炎患者均不同程度存在优势菌群失调。2.不同分期的UC患者肠道优势菌群的改变与UC的活动性具有相关性。更多还原

【Abstract】 Objective:Studying the alteration of intestinal flor in active ulcerative colitis, ulcerative colitis in remission patinents and the change of pathology for Mongolian in Chifeng region.To explor the relationship between the change of ulcerative colitis (UC)intestinal flora and occurrence and development of disease. To provide the basis for clinical treatment of UC.Methods:Case collection and analysis method of Fecal Flora1.Collecting 32 cases Mongolian (UC) Ulcerative Colitis patients who were treated from two third level hospitals from October 2008 to December 2009, in accordance with Sourtherland DAI,individed into two groups,UC active and UC remission.2.Choosing 18 Mongolian cases of physical examination from our hospital as control group.3.Collecting excrement of the cases and control groups for Enterobacteria,Enterococcus,Staphylococcus, Yeast,Bacteroides,bifidobacte rium,peptococcus,lactobacillus, eubacterium and Clostridium,above 10 kinds of intestinal predominant flor make quantitative analysis of the flora.Results1. Comparing with the control group, the quantity of Enterobacteria (9.00±0.19, p<0.01) in the Intestinal Flora with active (UC) Ulcerative Colitis patients, Enterococcus (8.04±0.38, p<0.01) increases obviously,the quantity of fusiformis (6.83±0.34, p<0.05) increase slightly,the quantity of Bacillus Bifidus (7.34±0.29, p<0.01) decrease obviously and other Bacteriums do not change obviously.2. Enterobacteria (8.54±0.24, P<0.05) in remission (UC) Ulcerative Colitis patinents is the trend of increasment, the quantity of Bacillus Bifidus and Eubacterium decrease slightly,but it is not obvious difference. Other Bacterias do not change obviously.Conclusions1, The Mongolian Ulcerative Coliti patients of ChiFeng exist flora imblanceIn different degrees.,2, the different stages (UC) Ulcerative Colitis patinents, it has the relevant connections between the UC patients with changes in intestinal predominant flora and the activity of UC.更多还原