低温复合产絮菌去除铁离子的试验研究

【作者】 刘泽群；

【导师】 韩晓云；

【作者基本信息】 黑龙江大学 ， 微生物学， 2010， 硕士

【摘要】 我国地下水资源中富含铁离子,为了提高对地下水的利用率,需要对其进行去除。本试验利用从活性污泥和低温生物反应器中筛选得到的四株低温产絮菌,H8、H13、DN1、DG2对水中的几种金属离子进行去除研究,发现这四株菌对铁离子的去除效果最好。经过进一步菌种复配,得到由四株产絮菌组成的复合菌对水中铁离子的去除率达到75%以上。为了得到对铁离子去除高效的复合絮凝菌,对其培养条件进行优化,结果表明当向pH8.0的培养液中接种2%的复合菌后,在20℃,100r·min-1的摇床中连续培养72h,培养出的复合菌对铁离子的去除效果最好,去除率达到80%以上。对培养得到的复合絮凝菌进行去除铁离子的静态试验研究,研究结果表明：试验水样pH值为6.5至7.0时,向含有10mg·L-1铁离子的试验水样中投加4%的复合絮凝菌,DO3.0mg·L-1,曝气5h后,复合絮凝菌对水中铁离子的去除率可达到80%以上。进行除铁动态试验研究过程中,为了避免复合絮凝菌的流失,需要对复合菌进行固定化。固定化试验研究后得到的最佳固定化条件为：海藻酸钠包埋剂的浓度为4%,低温复合絮凝菌与包埋剂的重量比为1：2,用直径为5mm的医用针头将混合液滴入浓度为1%CaCl2交联剂中,交联18h,得到的凝胶小球能够满足试验和应用要求。将复合絮凝菌固定化小球投加到除铁试验装置中,进行装置运行参数和效果优化,试验表明：试验装置中的水样pH为6.5-7.0,向其中投加5%的复合絮凝菌固定化小球,DO3.0mg·L-1条件下运行,复合菌对铁离子的去除率可达到80%以上。运行30d时,复合絮凝菌对铁离子的去除率达到84%以上。由此可以推断,本试验的动态运行系统在经过较长时间的运行后,运行效果良好,对铁离子的去除能力较强,可为除铁工艺工程化进程奠定一定的理论和实践基础。更多还原

【Abstract】 In China the groundwater resources contains abundant of iron, which needs to be removed in order to improve the utilization of ground water.In this experiment, four bacteria strains of low-temperature flocculant-producing (strain H8, H13, DN1, and DG2).which were screened from activated sludge and low temperature bioreactor were investigated in removing several metal ions in water. The study found that the iron ions could be removed effectively by four bactria strains. After the further rematch these four strains, obtained the compound flucculanting bacteria, which is constituted by H8, H13, DN1, and DG2. By using the compound flocculant-producing bacteria, the removal rate of iron in the water was over 75%.In order to improve the iron removal efficincy, the culturing conditions were optimized, including culturing temperature, pH, culturing time, and vaccinating amount.. The results showed that the best culturing condition was:after vaccinated 2% of compound flocculant-producing bacterium into the culture solution which is pH8.0, and cultivated 72h at 20℃, rotating 100r·min-1. The effective of removing iron is best by using the compound flocculant-producing bacterias cultrued under the best conditions; the removal rate of iron was over 80%.Investigating the iron removing conditions by static test, the results showed the best removing effect was:vaccinated 4% of compound flocculant-producing bacterium into the water, the water contained 10mg·L-1 iron which pH is from 6.5 to 7.0. After 5h aeration, DO3.0 mg·L-1, the removal rate of iron was over 80%.In order to avoid the bacteria running off, compound flocculant-producing bacteria were needed to be immobilized. The condition of immobilization was studied and was determined that the concentration of sodium alginate embedding medium was 4%, the proportion of the low temperature compound flocculant-producing bacterium and embedding medium was 1:2, dropping the mixed liquor into CaCl2 linker which concentration was 1%, cross linking 18 hours. The gel glocule of compound flucculanting-producing bacteria was obtained which was able to apply to iron removal.The immobilized globule of compound flocculant-producing bacteria was applied into the deferrization test facilities designed for iron removal treatment. Running parameter of the treatment was optimized and the results indicated that by putting 5% fossilize globule of compound flocculant-producing bacterium into the water, pH was 6.5-7.0, DO3.0mg·L-1, the removal rate of iron was over 80%.After 30 days running, the removal rate of iron reached to 84% by using the compound flocculant-producing bacteria. The iron removal effect was proved high effiency by compound flocculant-producing bacteria after immobilization and the treament effect could last for long time. The results above can settle the theory and practice foundation in deferrization technology project.更多还原