【作者】 张义冉；

【导师】 汪恩强；

【作者基本信息】 河北农业大学 ， 临床兽医学， 2010， 硕士

【摘要】 为治疗奶牛子宫内膜炎,本试验以河北省邢台市3个大型牛场饲养的奶牛为调查对象,对20头患病奶牛子宫分泌物通过显微镜检查、细菌分离培养、生化鉴定等方法进行病原学研究。并在此基础上,通过子宫灌注致病性大肠杆菌、葡萄球菌、链球菌、沙门氏菌、蜡状芽孢杆菌等混合菌液,复制獭兔子宫内膜炎病理模型。运用正交试验的方法优选栓剂制备方案,以栓剂的色泽、不溶物、融变时限为标准,研制奶牛子宫内膜炎中药栓、复方碘栓和生物栓。对獭兔子宫内膜炎病理模型分别于第1d,3d,5d按0.2g/kg﹒bw子宫投放中药栓,复方碘栓,生物栓进行治疗,观察其用药前后的临床症状、子宫形态、子宫内膜病理学变化,并测定血清SOD,MDA,GSH-Px等生化指标。对30头患子宫内膜炎奶牛分别子宫投放三种栓剂,观察其用药前后临床症状,并于第0d、2d、4d、6d、8d测定血清SOD、GSH-Px活力和MDA含量。结果表明,从患病奶牛子宫分泌物中共检出6种病原菌,其中金黄色葡萄球菌8株(占34.78%)、表皮葡萄球菌6株(占26.09%)、链球菌2株(占8.70%)、沙门氏菌2株(占8.70%)、大肠杆菌4株(占17.40%)、蜡状芽孢杆菌1株(占4.35%)。制备出的中药栓,复方碘栓,生物栓符合药典标准。对獭兔子宫内膜炎病理模型治疗试验结果,与各组首次用药前各项指标相比,复方碘栓投药4d后,阴门肿胀及阴道充血逐渐消退,子宫分泌物逐渐减少,子宫颈和子宫角轻微肿胀,宫腔内有少量透明分泌物,子宫内膜较完整,上皮细胞结构基本正常,血清SOD和GSH-Px活力均显著增高(P<0.05);6d后血清SOD活力显著增高(P<0.05),而血清GSH-Px活力极显著增高(P<0.01),血清MDA含量极显著降低(P<0.01)。中药栓治疗组和生物栓治疗组临床症状均未有明显改善,血清SOD、GSH-Px活力和MDA含量变化也不明显。对30头患子宫内膜炎奶牛进行临床治疗试验结果,给药后患病奶牛阴门分泌物明显减少。第6d复方碘栓治疗组SOD活力极显著性增高(P<0.01),血清GSH-Px活力显著性增高(P<0.05),血清MDA含量极显著性降低(P<0.01)。第8d生物栓治疗组血清SOD活力显著性增高(P<0.05),血清GSH-Px活力极显著性增高(P<0.01),血清MDA含量极显著性降低(P<0.01);而中药栓治疗组血清GSH-Px活力显著性增高(P<0.05),MDA含量极显著性降低(P<0.01)。可见,复方碘栓是治疗子宫内膜炎的最佳药物。更多还原

【Abstract】 In order to treat the cow endometritis, 20 endometritis cows in 3 large farms of XingTai, Hebei province were selected, their uterus secretions were studied by microscopic examination, isolation and culture of pathogens, biochemical identification etc. Under this foundation, the rabbit endometritis pathological model was made by perfusion of pathogenic E. coli, staphylococcus, streptococcus, Salmonella, Bacillus cereus and other mixed bacilli to the uterus. The schemes of the suppository preparation were optimized by orthogonal test, the color, insoluble substance, melt time were used as standard to prepare the traditional Chinese medicine suppository, the compound iodine suppository and the biological suppository. The suppositories were put to the uterus by 0.2g/kg﹒bw in 1st, 3rd, 5th days respectively, the clinical symptoms, uterine shape, pathological changes of endometrium, SOD, MDA, GSH-Px in sera, and other biochemical parameters were observed. The suppositories were put into the uterus of 30 endometritis cows respectively. Clinical symptoms were observed, and serum SOD, MDA, GSH-Px were examined in 0d, 2d, 4d, 6d, 8d treatment.The results showed that 6 pathogens were isolated in the uterus secretions, they were Staphylococcus aureus(8, 34.78%), Staphylococcus epidermidis(6, 26.09%), Streptococcus(2, 8.70%), Salmonella(2, 8.70%), E coli(4, 17.40%), Bacillus cereus(1, 4.35%) respectively. The traditional Chinese medicine suppository, the compound iodine suppository and the biological suppository were fit the standard of the pharmacopoeia. Compared with the indexes before using the suppositories, the results of rabbit endometritis pathological model after using the suppositories showed that the vulva edema and vaginal congestion subsided gradually, the uterine secretions reduced gradually. The uterine cervix and horn swelled slightly, there was a small amount of clear secretions in the uterus. The endometrium was more integrity, the structure of epithelial cell was normal. SOD and GSH-Px activity in sera were increased significantly (P<0.05) by 4 days treatment of compound iodine suppository. Serum SOD activity was increased significantly (P<0.05), serum GSH-Px activity was increased very significantly (P <0.01), serum MDA content was decreased very significantly (P <0.01) by 6 days treatment of compound iodine suppository. There were no significant change of the clinical symptoms, the serum SOD, GSH-Px and MDA in the traditional Chinese medicine suppository and biological suppository groups. The results on cow endometritis after using the suppositories showed that the uterine secretions of cows were obviously decreased. In 6d treatment, serum SOD activity increased significantly(P<0.01), serum GSH-Px activity increased significantly(P<0.05), and serum MDA content decreased significantly (P<0.01) in the compound iodine suppository groups. In 8d treatment, serum SOD activity increased significantly (P<0.05), serum GSH-Px activity increased significantly(P<0.01), and serum MDA content decreased significantly(P<0.01) in the biological suppository groups. While serum GSH-Px activity increased significantly (P<0.05) and serum MDA content decreased significantly (P<0.01) in the traditional Chinese medicine suppository groups. The compound iodine suppository was optimized in the suppositories for treatment of endometritis.更多还原