【作者】 宋杰；

【导师】 张英杰；

【作者基本信息】 河北农业大学 ， 动物营养与饲料科学， 2010， 硕士

【摘要】 选择4~5月龄、体重25.4kg左右的杂交羔羊36只,随机分为3组:低能量组、中能量组、高能量组,每组12只。中能量组按照中国肉羊饲养标准(NY/T816-2004)中的能量需要量设计饲喂日粮,能量水平为10.33MJ/d,低、高能量组处理是在中等能量水平的基础上分别减少或增加30%。饲喂37d后,屠宰取绵羊心脏、背最长肌和股二头肌组织样,用荧光定量PCR法对绵羊不同组织H-FABP mRNA表达量进行检测,分析不同能量水平对H-FABP mRNA含量的差异。同时取绵羊背最长肌和股二头肌组织样,应用常规分析方法测定肉品质的常规指标。试验结果如下:1羊肉系水力、pH值、剪切力及肌间脂肪含量与日粮能量水平有关。中能量组失水率、pH值、剪切力与低能量组相比,差异均不显著(P>0.05)。高能量组失水率、pH值与中能量组相比,差异不显著(P>0.05)。高能量组失水率与低能量组差异极显著(P<0.01)。高能量组背最长肌pH值与低能量组差异显著(P<0.05);高能量组股二头肌pH值与低能量组差异极显著(P<0.01);高能量组背最长肌剪切力显著低于中、低能量组(P<0.01);高能量组股二头肌剪切力显著低于低能量组(P<0.01),高与中能量组差异不显著。低能量组背最长肌及股二头肌肌内脂肪含量极显著低于中、高能量组(P<0.01),中、高能组之间差异也极显著(P<0.01)。2.不同组织中H-FABP mRNA含量均随着能量水平的提高而增加,背最长肌和股二头肌高、中、低能量水平之间差异均极显著(P<0.01)。高能量组心脏组织H-FABP mRNA含量极显著高于中、低能量组(P<0.01);中、低能量水平之间差异显著(P<0.05)。3. H-FABP mRNA含量具有组织差异性。背最长肌和股二头肌之间H-FABP mRNA无显著差异(P>0.05),心脏组织中H-FABP mRNA含量极显著地高于背最长肌和股二头肌(P<0.01)。更多还原

【Abstract】 Choose healthy disease-free, 4~5 months old , weight 25.4kg of hybrids lambs which were randomly divided into 3 groups of 12,including group A (low-energy group), group B(middle-energy group),group C (high-energy group). Group B was raised according to Chinese sheep raising standards (NY/T816-2004) design requirements in the diet of protein, the metabolic energy was 10.33 MJ / d, group A and group C based on middle-energy group on the reduction and increase of 30%,separately. After 37 days feeding, slaughtering sheep and taking heart, longissimus dorsi and biceps femoris muscle tissue samples, using real time quantitative PCR method to detect the expression of H-FABP mRNA in different tissues of sheep and analyze of different energy levels on the H-FABP mRNA content differences. At the same time, taking the longissimus and biceps femoris to determine the main indicators which affect the meat quality by conventional analysis methods. The results are as follows:1.The department of water, pH value, shear force and intramuscular fat content is relevant to the level of dietary energy.Compared with the low-energy group,the loss rates of water, pH value and shear force of the the middle-energy group is not significantly difference(P>0.05).The difference of the loss rates of water and pH value between the high-energy group and the middle-energy group is not significantly. The loss rates of water between the high-energy group and the low-energy group is significantly difference(P<0.01).The pH value of longissimus between the high-energy group and the low-energy group is significantly difference(P<0.05); the pH value of biceps femoris muscle between the high-energy group and the low-energy group is significantly difference(P<0.01). The shear force of longissimus of the high-energy group is significantly lower than that of the middle-energy group and the low-energy group(P<0.01); the shear force of biceps femoris muscle of the high-energy group is significantly lower than that of the low-energy group(P<0.01),and there is on difference between the high-energy group and the middle-energy group. The intramuscular fat content of longissimus and biceps femoris muscle of the low-energy group is significantly lower than that of the middle-energy group and the high-energy group(P<0.01),and the difference between the middle-energy group and the high-energy group is also significantly different(P<0.01).2.The content of H-FABP mRNA in different organism is rised as the energy level increased, it is significantly difference among the low-energy group, the middle-energy group and the high-energy group in longissimus and biceps femoris muscle(P<0.01). The content of H-FABP mRNA in cardiac tissue of the high-energy group is significantly higher than that of the low-energy group and the middle-energy group(P<0.01); it is significantly difference between the low-energy group and the middle-energy group(P<0.05)3.The content of H-FABP mRNA is variability in different organism. The content of H-FABP mRNA is not significant difference in longissimus and biceps femoris muscle(P>0.05),but the content in cardiac tissue is significantly higher than in longissimus and biceps femoris muscle(P<0.01).更多还原