【作者】 柳洪良；

【导师】 吴明根； 崔宗均；

【作者基本信息】 延边大学 ， 作物栽培与耕作学， 2010， 硕士

【摘要】 甘蔗渣资源占我国生物质资源的重要组成部分,由于其自身高纤维素含量的特点,一直以来被广泛应用于建材等方面广为应用,但作为饲料资源的应用报道较少。由于甘蔗渣具有养分低,适口性差,消化率低等特点,直接饲喂动物采食量低,因此必须寻找新的途径解决该问题。如果能提高其进食率并提高其营养成分,甘蔗渣作为反刍动物的饲料便成为缓解人畜争粮问题和增加饲料品种来源的重要途径,也符合我国畜牧业长远发展的目标。本研究从筛选接种于甘蔗渣发酵的乳酸菌复合系出发,筛选出较稳理想的甘蔗渣发酵菌剂LBC-8,并对乳酸菌复合系LBC-8的组成进行了16SrDNA系统发育分析,即菌体克隆。结果显示其主要组成菌为：乳酸杆菌(Lactobacillus sp)、发酵乳杆菌(Lactobacillus fermentum)、(Lactobacillus pantheris)、植物乳杆菌(Lactobacillus plantarum)、干酪乳杆菌(Lactobacillus casei)、副干酪乳杆菌(Lactobacillus paracasei)和醋酸菌(Acetobacter pasteurianus)等组成。在将乳酸菌复合系LBC-8直接接种到甘蔗渣后发现,复合系菌群能够占主导地位成为优势菌株。通过甘蔗渣起始糖浓度对甘蔗渣发酵饲料品质的影响进行研究。结果表明,甘蔗渣起始糖浓度5.0%DM(干物质)为甘蔗渣能否成功发酵的分界线,通过提高甘蔗渣糖浓度可以在一定程度上改善发酵饲料的品质；DGGE结果证明,当甘蔗渣起始糖浓度达到5.0%以上时,乳酸菌增殖明显。在研究可溶性糖对甘蔗渣乳酸发酵影响的基础上,还进行了适合甘蔗渣发酵饲料喷施的方式及保存的方法的选择。通过相关性质分析及DGGE结果证明,在不同的喷施菌液的方式及保存发酵饲料的方法在结果上有很大的差异,以喷淋菌液并密封保存的方法效果最佳。通过对乳酸菌复合系的筛选及性质的研究,并接种于甘蔗渣进行发酵饲料的制作,使微生物菌剂用于生物质资源的开发和利用又向前迈进了一步,并且使甘蔗渣微生物饲料领域更为丰富化。为以后的实际生产和应用打下了坚实的基础。更多还原

【Abstract】 Bagasse resources were an important part of China’s biomass resources, it has been widely used in building materials and so widely used because of the high cellulose content of its own characteristics. we must seek new ways to solve the problem that the bagasse with low nutrients, poor palatability, low digestibility and low intake by direct-fed animal. It was an important way that as a ruminant animal feed has become an indisputable human and animal food to alleviate the problems and increase the variety of sources if the consumption rate and the nutrition of bagasse can be increased, it also in line with the long-term development objectives of China’s animal husbandry.This study from the screening the lactic acid bacteria community of bagasse fermentation starting, and filter out the more appropriate lactic acid bacteria community LBC-8, and also made the analysis of bacterial composition of LBC-8 with the 16S rDNA phylogenetic analysis(bacterial clone). The results showed that the bulk of their strains as follows:Lactobacillus sp, Lactobacillus fermentum, Lactobacillus pantheris, Lactobacillus plantarum, Lactobacillus casei, Lactobacillus paracasei and Acetobacter pasteurianus.The lactic acid bacteria community LBC-8 inoculation into the bagasse and found that the community can be the dominant to become predominant strains.By the reseach of effects of different soluble sugar concentration on fermentation characteristics of bagasse found that water soluble sugar concentration 5.0% DM can be success of the fermentation of bagasse and improvong the quality of bagasse fermentation. DGGE results show that lactic acid bacteria obvious proliferation when the water soluble sugar concentration 5.0% DM.We made a choice in the spraying methods and preservation options of fermented bagasse on the basis of effects of water soluble sugar concentration on fermentation characteristics of bagasse. From the patterns of denaturing gradient gel electrophoresis (DGGE), there were different results in a different way of spraying and the preservation methods in fermented feed, the best method is sprayed sealed way.It maked the microbial agents used in the development of biomass resources and made great progress by the screening of lactic acid bacteria and the nature studies of the community and inoculated in the bagasse for the production of fermented feed, and to make more abundant of the field of microbial feed, and also laid a solid foundation for the practical production and application.更多还原