【作者】 张喜娟；

【导师】 严祥；

【作者基本信息】 兰州大学 ， 临床医学， 2010， 硕士

【摘要】 目的：研究糖尿病胃动力变化与胃血管变化的关系。方法：72只SPF级雄性Wistar大鼠适应性喂养一周,随机分为实验组和对照组,实验组动物采用高脂高糖饲料喂养诱导并小剂量链脲佐菌素(STZ)注射破坏胰岛β细胞,制造2型糖尿病模型。分别于4周,24周时应用单光子发射断层显像仪(SPECT)技术测定胃排空,观察血内皮素(ET-1)、血栓调节蛋白(TM)、血管性假性血友病因子(vWF),并在显微镜下观察胃血管的病理变化。结果：24周模型组大鼠血浆TM、vWF、ET-1显著升高,4周模型组轻度升高。显微镜观察见早期血管内皮轻度损害,微血管代偿性增加,胃动力加快。随着病程进展,血管内皮损害进一步加重,胃粘膜微血管逐渐减少,毛细血管扩张,粘膜下及肌层小血管管壁增厚,管腔狭窄,管壁／管腔比值明显增大,胃动力延缓。结论：糖尿病胃动力变化与胃血管变化相关,并且血管病变可能是引起胃动力变化的始动因素。更多还原

【Abstract】 Objective:To investigate the correlative of the gastric motility change and gastric vasal change in type 2 diabetic rats. Methods:72 male SPF Wistar rats were first divided random into two groups:control (n=24) and diabetic group (n=48). The control was fed ordinary laboratory diet for 20 weeks, but the diabe-tic group was fed fat-and caloric-rich laboratory diet for 6 weeks and then inject-ed with small dose of streptozotocin(30mg/kg), until 24weeks to develop diabet-ic gastric motility disorders model. Gastric emptying was measured by SPECT after 4,24weeks either. In addition observing plasma endothelin (ET-1), thromb-omodulin (TM), vWillebrand factor (vWF), and observed under a microscope, the gastric peripheral vascular and microvascular pathological changes. Results: The plasma TM, vWF, ET-1 increased significantly in 24 weeks and increased slightly in 4 weeks. Early microvascular compensatory increase, and gastric motility speed up. As the illness progresses, gastric microvascular diminishing、telangiectasia、small vessel wall thickening in submucosal and muscular layers、stenosis、wall/lumen ratio significantly increased. Conclusion:The vascular changes associated with changes in gastric motility in diabetes.更多还原