【作者】 李文海；

【导师】 袁呈山；

【作者基本信息】 兰州大学 ， 有机化学， 2010， 硕士

【摘要】 箭舌豌豆(Vicia sativa linn),属于蝶形花科(Papilionaceae),野豌豆属(Vicia)一、二年生或多年生草本。野豌豆属含物种约200种,产于北半球温带至南美洲温带。我国有43种5变种,广布于全国各省区,西北、华北、西南较多。此种为绿肥和及优良牧草。全草药用。花果及种子有毒,国外曾有用其提取物作抗肿瘤的报道。京大戟(Euphorbia pekinensis Rupr),系大戟科(Euphorbiaceae)大戟属(Euphorbia)多年生草本植物。大戟科植物主要产于热带或亚热带,约包含300个属8000余种,在我国大戟科约有66属360余种,主要分布于长江流域以南各省区。该科植物的主要化学成分为生物碱、二萜及三萜类化合物。多数植物含有抗癌,抗菌,抗病毒等生理活性的二萜类化合物。为进一步挖掘箭舌豌豆和京大戟中新颖的化合物,本论文对箭舌豌豆和京大戟两种植物的化学成分进行了系统研究,采用常规的硅胶柱层析(CC),葡聚糖(Sephadex LH-20)柱层析,制备薄层层析(PTLC)和重结晶等实验手段对其进行了分离纯化,利用波谱技术(Ir, EI-MS,HR-ESI-MS,1H NMR,13C NMR,DEPT, 1H-1H COSY, HMBC, HSQC等)对分离的化合物进行了结构鉴定。从箭舌豌豆(Vicia sativa linn)中分离得到了10个化合物,1个为新化合物；从京大戟(Euphorbia pekinensis Rupr)中分离得到了20个化合物,其中3个为新化合物。一个新的天然产物。此外,为了寻找新的活性成分,我们对分离得到的部分化合物进行了细胞毒活性实验。论文最后对大戟科植物近年来的研究概况和生物活性研究进行了综述。更多还原

【Abstract】 The Vicia sativa linn, a perennial herb, is genus of family Papilionaceae. There are about 200 species all over the world which are widely distributed in Northern Hemisphere temperate regions and South America. In China, there are 43 species and 5 mutations which are widely spreaded all across the country, espacially in Northwest, North and Southeast. They have long been used as green manure, also are fine pastures. Previous studies on Vicia sativa linn indicated that it contained flavonol, flavonol glycoside, steroid and terpenes. It is also reported that its extract was ever used to treat tumor.The Euphorbia pekinensis Rupr is a genus of family Euphorbiaceae tribe Euphorbia and a perennial herb. There are about 8000 species all over the world which are widely distributed in tropic or subtropic zone. The genus Euphorbia is composed of more than 360 species in China which are widely spreaded in provinces south of Yangzi River. The major actively functional constituents include alkaloid, diterpenoids and triterpenoids. Research shows diterpenoids have anti-tumor, antibiosis, antiviral activies.In order to explore more medical potential plants of Vicia species and Euphorbia species, and to exploit new resources of Chinese traditional medicine, the chemical constituents of these two plants have been studied. The compounds were isolated by means of silica gel and Sephadex LH-20 gel columm chromatography, preparative thin layer chromatography and recrystallization etc. The structures of these compounds were elucidated by spectroscopic methods (IR, EI-MS, HR-ESI-MS, 1H NMR,13C NMR, DEPT,’H-’H COSY, HMBC, HSQC etc.). From Vicia sativa linn, ten compounds were isolated and elucidated, one of them is new compound, and twenty compounds were isolated and elucidated from Euphorbia pekinensis Rupr and three of them were new compounds, one triterpene was isolated from nature for the first time. Meanwhile, some compounds were tested for cytotoxic activity the result indicated that one of them exihibited high cytotoxic activity.Finally, studis on chemical constituents of Euphorbiaceae were reviewed in this thesis.更多还原