【作者】 许琳雅；

【导师】 周光雄；

【作者基本信息】 暨南大学 ， 药物化学， 2010， 硕士

【摘要】 本论文对两株来源于我国南海红树林底泥且具有抗菌活性的海洋放线菌(No.H74-18和No.H41-26)的发酵液和菌丝体进行了活性成分的分离和结构鉴定。利用硅胶开放柱色谱、ODS开放柱色谱、制备性HPLC和葡聚糖LH-20排阻层析等多种色谱方法,从活性流分中分离得到得到21个单体样品、1个结晶和1个混晶,经MS、1D及2D-NMR或X-衍射等谱学方法鉴定了其中12个化合物结构。它们分别为抗霉素类化合物：抗霉素A1a(1)、抗霉素A1b(2)、抗霉素A2a(3)、抗霉素A3a(4)、抗霉素A3b(5)、(2S,3R,6R,8S)-3-(3-formamido-2-hydroxybenzamido)-8-hexyl-2,6-dimethyl-4,9-dioxo-1,5-diox onan-7-yl acetate(抗霉素A18,6)、(2S,3R,6R,8S)-3-(3-formamido-2-hydroxybenzamido)-2,6-dimethyl-4,9-dioxo-8-pentyl-1,5-dioxonan-7-yl-2,3-dimethylbutanoatei(抗霉素A19,7)；放线菌素类化合物：放线菌素D(8)、放线菌素X2(9)、actinomycin放线菌素X0β(10)、放线菌素X0δ(11)；其他类型化合物：吡虫啉(1)。其中化合物6、7为新天然产物。更多还原

【Abstract】 This thesis has studied on the isolation and structural determination of active constituents in fermented mycelium and broth from two strains of marine actinomycetes No. H74-18 and No. H41-26, which were isolated from the bottom soil of mangrove zone in south China sea and showed antibiotic activity. Twenty-one pure samples, one crystal sample and one mixed crystal sample were isolated from the active fractions by silica gel column, ODS open column chromatography, preparative HPLC, Sephadex LH-20 permeation chromatography, and other chromatographic techniques. Twelve compounds were identified by MS, 1D and 2D NMR spectroscopic analysis, or X-ray diffraction analysis, they were determined to be 7 antimycins as antimycin A1a(1), antimycin A1b(2), antimycin A2a(3), antimycin A3a(4), antimycin A3b(5), (2S,3R,6R,8S)-3-(3-formamido-2-hydroxybenzamido)-8-hexyl-2,6-dimethyl-4,9-dioxo-1,5-diox onan-7-yl acetate (antimycin A1g,6),(2S,3R,6R,8S)-3-(3-formamido-2-hydroxybenzamido)-2,6-dimethyl-4,9-dioxo-8-pentyl-1,5-dio xonan-7-yl 2,3-dimethylbutanoate (antimycin A19,7).4 actinomycins as actinomycin D (8), actinomycin X2 (9), actinomycin X0p (10), actinomycin X0s(11), and imidacloprid (12). Compounds 6 and 7 were two new natural products..更多还原