【作者】 刘斌；

【导师】 向军俭； 唐勇；

【作者基本信息】 暨南大学 ， 免疫学， 2010， 硕士

【摘要】 目的：规范重金属镉、铅残留样品的处理流程,建立适应于重金属镉、铅免疫学快速检测的样品处理与富集方法,并研制成质量可靠稳定的免疫学检测试纸条。方法：首先对含有重金属Cd、Pb残留的样品进行采集,包括水样、土壤、植物、农产品类等。针对不同样品,选择不同的条件进行预处理,如水样进行过滤、土壤进行混酸微波消解等,然后选用纳米Ti02对处理液中的重金属Cd、Pb进行分离富集,摸索分离富集的最佳条件。最后应用胶体金试纸条方法进行Cd、Pb的含量检测,摸索免疫检测试纸条的最佳检测条件。结果：针对水样中的重金属Cd、Pb残留,已经形成完整的样品处理及免疫学检测流程。结果表明,将采集好的水样用0.45μm滤膜过滤,调节pH至9.0,水样中残留的Cd可被纳米Ti02定量富集,吸附于纳米TiO2上的镉离子可用0.1 mol/L的EDTA·2Na溶液定量脱附。在优化实验条件下,纳米Ti02对Cd的吸附容量为14.7 mg/g,富集倍数可达50倍。制备了比色法判定结果的胶体金试纸条,并建立了纳米Ti02富集-胶体金试纸条联用检测方法。对实测样品的检测耗时约90min,该方法对Cd的定量下限可达5μg/L,适用于环境水样检测的国家标准。同样,水样中的残留的Pb可被纳米Ti02定量富集,然后用DTPA溶液进行洗脱,富集倍数可达50倍。制备了比色法判定结果的胶体金试纸条,对分离富集后的溶液进行Pb含量检测,换算倍数后,得出的检测结果令人满意,符合国家检测标准。结论：成功建立了重金属Cd、Pb残留的纳米钛富集－免疫学检测的方法,该方法简便,易于推广,为现在重金属的残留检测提供了新思路新方法,为重金属免疫学检测方法的实际应用奠定了基础。更多还原

【Abstract】 Objectives:To standardized the sample processing against cadmium and lead, to establish a method of sample handling and Enrichment for the Immunological detection against ion cadm ium and lead, and make stable and reliable Colloidal Gold test papers.Methods:First of all,samples must be collected including water, plants,soil and primary pro ducts,and choose different qualification to handle different kinds of samples. Then,it could using Nanometer-size TiO2 to adsorp the ion cadmium and lead,as well as explore the best conditions of Adsorption. Finally, colloidal gold immunochromatographic assay was used for detecting ion cadmium and lead, and the best conditions of Immunological detection was fixed.Results:At pH 9.0, Cd was retained on the nanometer-size TiO2, then eluted with 0.1 mol/ L EDTA·2Na quantitatively. The adsorption capacity of nanometer TiO2 was found to be 14.7 mg/g for Cd(II). The maximum preconcentration factor was 50. We made a kind of dipstick wit h colorimetric assay, nano colloidal gold particles were prepared and labeled to an anti-Cd(Ⅱ)-i EDTA (Isothiocyanobenzyi-EDTA) monoclonal antibody. Linked with nanometer TiO2 as soli d adsorbent, the whole detection process could be performed in 90 minutes, and the minimum de tection concentration could be down to 5μg/L. Samely, Pb was retained on the nanometer-size Ti 02, then eluted with 0.5 mol/L DTPA quantitatively. The maximum preconcentration factor also was 50. The method was applied to the determination of Cd (Ⅱ) and Pb(Ⅱ) in environmental wa ter samples and satisfactory results were obtained.Conclusion:A new method was developed for the rapid determination of trace Cd(Ⅱ) and Pb(II) using nanometer sized TiO2 as solid adsorbent and detected by Colloidal gold immunoch romatographic method. The sensitivity of the method is high enough to satisfy the safety require ments for non-environmental pollution aquatic products.更多还原