【作者】 王乐；

【导师】 李多伟；

【作者基本信息】 西北大学 ， 细胞生物学， 2010， 硕士

【摘要】 绿原酸是一种天然的抗氧化剂,具有免疫调节、抗癌抗衰老等作用,同时还被认为是很有希望的抗艾滋病毒先导化合物。目前我国绿原酸产业不发达,仍需进口部分绿原酸产品特别是高纯度制品才能满足市场需求,因此急需一种低成本、高得率的绿原酸生产工艺,开展此方面相关研究具有较高的经济价值和广阔的市场前景。我国传统植物元宝枫中富含绿原酸,本文即以元宝枫叶为提取原料,开发一种能降低绿原酸生产成本、提高产物纯度与得率,并减少废弃物污染的工艺,为发展完善绿原酸产业提供理论依据,使其进一步市场化。研究结果如下：1分别使用石油醚和乙酸乙酯对元宝枫叶原料进行热回流提取预处理,结果证明其可优化提取工艺流程效率,有利于提高绿原酸产品纯度。2对比数种提取方法之间的效率,根据结果确认使用超声波法提取元宝枫叶中绿原酸的。针对其设计单因素考察试验与正交试验,优选得出超声提取元宝枫叶中绿原酸的最佳参数：超声功率150W,提取时间15 min,料液比1：12(m／v),提取2次。使用此方法绿原酸得率达3.14%。3采用大孔树脂层析法分离纯化元宝枫叶提取液中的绿原酸,通过对4种不同类型树脂的筛选发现LSA-21树脂具有较高的吸附率和解吸率,用Langmuir方程分析得出其对绿原酸的理论最大吸附量可达90.91 mg/g,4h内可基本吸附完全。进一步研究确定最佳分离纯化工艺条件为：pH 3.0,以8BV(柱体积)绿原酸含量约为2 mg／ml的元宝枫叶提取液上柱吸附,6BV70%乙醇进行洗脱。4本实验选用反相高效液相色谱法进行测定,绿原酸的保留时间在7 min左右,与杂质色谱峰之间分离良好,且峰面积与绿原酸含量的线性关系符合定量分析原则,能较好满足精确测定的要求。经检测,按试验确定最佳工艺路线处理所得终产物中绿原酸纯度为78.14%,得率2.81%,绿原酸总回收率为73.48%。此方法效果优于传统工艺,缩短了生产周期,降低了生产成本,符合绿原酸产业化要求,值得大力推广。更多还原

【Abstract】 Chlorogenic acid, a kind of natural anti-oxidant, has an immuno-modulatory, anti-aging and anti-cancer effect. Moreover, it was considered as promising anti-HIV leading compounds. Recently, Chinese Chlorogenic acid industry hasn’t developed well. It is still essential to import some Chlorogenic acid products to meet market demands, particularly products in high purity. So there is an urgent need for a low-cost product process with high yield of Chlorogenic acid. Carrying out research in this area has high economic value and broad marketing prospects.Traditional Chinese plant Acer truncatum Bunge is rich in Chlorogenic acid. This paper is about developing a new product process of Chlorogenic acid with the maple leaves as the raw materials. It is hoped to reduce product costs, improve purity and yield of the product, reduce pollution by the waste. The aim of the paper is providing a theoretical basis for the industry of Chlorogenic acid to make the market of it more perfect. The results are as follows:1 Petroleum ether and ethyl acetate were used in heatened reflux extraction for pretreatment of the maple leaves. The results proved that it could optimize the efficiency of extraction process, improve the purity of Chlorogenic acid.2 According to the contrasting test results of some extraction methods, the ultrasonic method is chosen for the extracting of Chlorogenic acid. According to the result of single-factor and orthogonal test, the best program is:ultrasonic power is 150W, extracting time is 15 minutes, solid to liquid ratio is 1:12 (m/v), and extracting for 2 times. The yield of Chlorogenic acid with this method could reach 3.14%.3 Using macroporous resin chromatography to purify Chlorogenic acid from crude extracts of the maple leaves. Through the chosen test of 4 different types of resins, it is found that LSA-21 resin performed best. The analysis with the Langmuir equation proved its theoretical maximum of adsorption of capacity Chlorogenic acid is up to 90.91 mg/g, achieving in complete absorption in 4 hours. Further research confirmed the optimum conditions for separation and purification is:pH 3.0, using 8 BV (column volume) of crude extraction from maple leaves, with a content of Chlorogenic acid at about 2 mg/ml, for adsorption,6 BV of 70%ethanol for desorption.4 In this experiment, HPLC is used for determination of Chlorogenic acid. The retention time of it is about 7 minutes, with a good separation of the peaks of targeting products and impurities. The linear relationship between quantity of Chlorogenic acid and peak area has a consistency with the principles of quantitative analysis and can satisfy the requirements of precise determination. The test of experiment with the optimum producting process is as follows:purity of Chlorogenic acid product was 78.14%, with a yield 2.81%, a total recovery of 73.48%.This method is better than the traditional process, reducing the production cycle and cost, meeting the industrial requirements of chlorogenic acid, so worth promoting vigorously.更多还原