木聚糖酶的生产及应用研究

【作者】 李文孝；

【导师】 范代娣；

【作者基本信息】 西北大学 ， 化学工程， 2010， 硕士

【摘要】 本论文主要优化了木霉RS30(Trichoderma RS30)生产木聚糖酶的培养条件。并完成了木聚糖酶与纤维素酶对玉米秸秆的降解研究及含有木聚糖酶的复合酶影响蛋鸡生产性能的应用研究。通过优化试验获得产木聚糖酶的最佳条件是：温度为28℃、培养基pH值为5.0、种龄为96h、接种量1×107个孢子、摇床转速150r／min、培养时间144h。通过木聚糖酶和纤维素酶对玉米秸秆的降解研究,确定的酶解条件与效果为：在酶解体系pH4.5、温度50℃、固液比为1：15、摇床转速为100r／min、木聚糖酶120U／mL、纤维素酶60u／mL时,降解24h,玉米秸秆降解率为12.2%。蛋鸡饲料中添加复合酶制剂没有显著改变鸡蛋品质,使用了0.2%的复合酶制剂能有效提高蛋鸡的生产性能,平均产蛋率提高了2.53%,产蛋量提高了3.56%,料蛋比下降6.36%,盈利率提高了16.23%。更多还原

【Abstract】 In this study, the producing condition of xylanase by Trichoderma RS30 has been optimized. Then the xylanase was used for hydrolysis of corn straw cooperating with cellulase. An enzyme mixture containing xylanase was added into chick feed. The effect of enzyme mixture on eggs producing has also been investigated in this study.The optimal conditions of xylanase producing are as following:28℃, pH 5.0,96 hour-old inoculum, inoculum size of 1×107 spore, shake speed of 150 r/min, and 144 hours of fermentation.In the study on corn straw enzymatic hydrolysis, the performance of xylanase and cellulase mixture was evaluated. The highest degradation rate (12.2%) of corn straw was achieved under the conditions as following:pH 4.5,50℃, a solid to liquid ratio of 1:15, shake speed of 100 r/min, dosage of 120 U/mL xylanase and 60 u/mL cellulase,24 hours of hydrolysis.The enzyme mixture added into feeds has no significant effects on quality of eggs. The results indicated that addition of 0.2% complex enzymes in feed effectively improved the eggs production performance with 2.53% increase of average eggs producing rate,3.56% increase of eggs production,6.36% decrease of feed to egg ratio, and 16.23% increase of profile.更多还原