【作者】 杨喆；

【导师】 高云芳；

【作者基本信息】 西北大学 ， 动物学， 2010， 硕士

【摘要】 研究目的：1、观察不同浓度川芎嗪对吊尾大鼠比目鱼收缩功能及肌浆网Ca2+-ATP酶活性的影响。2、对川芎嗪影响大鼠比目鱼收缩功能的可能机制进行初步探讨。研究方法：1、尾部悬吊法建立大鼠废用性肌萎缩模型,使用PowerLab系统、电子刺激器(SEN.3301)等仪器测定不同浓度川芎嗪灌胃给药后,大鼠比目鱼肌肌肉收缩功能的变化。2、利用定磷法测定不同浓度川芎嗪给药后,吊尾大鼠比目鱼肌肌浆网Ca2+-ATP酶活性。研究结果：1、与吊尾组比较：1)不同浓度川芎嗪灌胃给药组中,大鼠比目鱼肌的等长、强直收缩最大张力值均有显著增加,其中高浓度组等长收缩最大张力值增长179.02%,强直收缩最大张力值增长90.29%,低浓度组等长收缩最大张力值增长186.65%,强直收缩最大张力值增长123.61%。高低浓度之间无显著差异；2)不同浓度川芎嗪组收缩时程的收缩相TP50、TPT参数均显著增加,高浓度组分别增长148.09%和80.28%,低浓度组分别增长95.38%和31.67%,高低浓度之间无显著差异。3)高浓度川芎嗪组收缩时程的舒张相RT50参数增加21.36%(P<0.05)。2、与吊尾组比较,灌胃给药川芎嗪后,高浓度组大鼠比目鱼肌肌浆网Ca2+-ATP酶活性升高55.60%(P<0.01),低浓度组升高71.84%(P<0.01)；不同浓度实验组中,低浓度组效果稍明显,但不具统计意义(P>0.05)。研究结论：1、不同浓度川芎嗪均能有效缓解吊尾造成的肌萎缩影响；有效对抗吊尾引发的比目鱼肌质网Ca2+-ATP酶活性降低。2、川芎嗪可通过提高肌浆网Ca2+-ATP酶活性、抑制肌纤维类型转换等途径,对抗废用对肌肉收缩功能的影响。更多还原

【Abstract】 Objectives:1, To observe the effects of different concentrations of TMP on the soleus’s contratility and on the sarcoplasmic reticulum Ca2+-ATPase activity in the tail-suspended rat.2, To discuss the possible mechanism of TMP’s effects on the soleus’s contractility in ratMethods:1, The disuse-atrophy model was achieved by tail-suspension on rat; using PowerLab system and electronic stimulator (SEN.3301) to testify the soleus’s contractility in rat.2, The sarcoplasmic reticulum Ca2+-ATPase activity was measured by the Phosphate Determination method.Results:1, Compared with the tail-suspended group:1) both the peak twitch tension (Pt) and peak tetanic tension (Po) of soleus in rat have increased significantly in those groups of intragastric administration with different TMP concentrations; the Pt and P0 have increased 179.02%, 90.29% respectively in the high TMP concentration group, and have increased 186.65%, 123.61% respectively in the low TMP concentration group; there was no significant difference between the high and low TMP concentration groups.2) the time to 50% peak tension (TP50) and time to peak tension (TPT) have incerased notably in those groups of intragastric administration with different TMP concentrations; the TP50 and TPT have rised 148.09% and 80.28% respectively in the high TMP concentration group, and have increased 95.38% and 31.67% respectively in the low TMP concentration group; no significant changes was observed between the high and low TMP concentration groups.3) time from peak tension to 50% relaxation (RTso) has increased 21.36%(P<0.05) in the high TMP concentration group.2, Compared with the tail-suspended group, after intragastric administration with TMP, the sarcoplasmic reticulum Ca2+-ATPase activity of the rat’s soleus has increased 55.60%(P <0.01) and 71.84%(P<0.01) in high and low TMP concentration groups respectively; low TMP concentration was more effective than high one on the sarcoplasmic reticulum Ca2+-ATPase activity but was not statistically significant (P>0.05). Conclusions:1, Different concentrations of TMP can effectively alleviate the Tail on the impact caused by muscle atrophy; effective against the Tail on the soleus muscle induced endoplasmic reticulum Ca2+-ATP activity decreased.2, TMP can increase sarcoplasmic reticulum Ca2+-ATP activity, inhibition of muscle fiber type conversion way, against the disuse of the muscle contraction function.更多还原