【作者】 柴伟；

【导师】 孙威；

【作者基本信息】 中国医科大学 ， 外科学， 2009， 硕士

【摘要】 目的探讨信号传导及转录活化因子3(STAT3)、生存素(Survivin)及血管内皮生长因子(VEGF)在胃癌组织中的表达及其与临床病理特征之间的关系。方法采用免疫组织化学SP法检测56例胃癌组织及其癌旁正常组织中STAT3和survivin及VEGF的表达情况。结果1、STAT3与Survivin及VEGF三者在56例胃癌组织中的表达阳性率分别为75.0%、71.4%及76.8%,均明显高于三者在癌旁正常组织中的表达率(X2=17.75-25.84,P<0.01)；2、三者在Ⅲ、Ⅳ期胃癌中的表达水平明显高于Ⅰ、Ⅱ期胃癌(P<0.001),胃壁浆膜受侵及、淋巴结有转移及低、未分化的胃癌中三者表达水平高于浆膜未侵及、淋巴结无转移及高、中分化的胃癌(P<0.05)；3、STAT3、Survivin及VEGF在胃癌组织的表达两两之间均表现出正相关关系。结论STAT3、Survivin及VEGF的过度表达与胃癌的发生发展密切相关,STAT3的活化导致其下游靶基因Survivin及VEGF的表达明显升高,促使肿瘤迅速增长、浸润及转移。更多还原

【Abstract】 ObjectiveTo investigate the expression of signal transducers and activators of transcription (STAT3), Survivin and vascular endothelial growth factor (VEGF) in gastric carcinoma and the relationship between clinicopathological features.MethodsImmunohistochemistry SP method was used to detect the expression of STAT3、survivin and VEGF in 56 cases of gastric carcinoma and adjacent normal tissue.Results1. The expression rate of STAT3、survivin and VEGF in 56 cases of gastric cancer tissue were 75.0%71.4% and 76.8% respectively, much higher than that cancer around tissue (X2= 17.75-25.84, P<0.01);2. The expression rate in phaseⅢandⅣof gastric cancer was much higher than that of cancer phaseⅠandⅡ(P< 0.01).The expression rate in serous membrane invasion of gasttic wall, lymph nodes metastasis, poorly differentiated and undifferentiated gastric cancer were higher than that of no membrane invasion,no metastasis and moderately and welldifferentiated gastric cancer (P<0.05).3. Positive correlation was found between the expression of STAT3、survivin and VEGF.ConclusionSTAT3, Survivin and VEGF over-expression is closely related to the genesis and development of gastric cancer, STAT3 activation leads to downstream target genes of Survivin and VEGF expression was significantly increased, prompting the rapid tumor growth, invasion and metastasis.更多还原