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纯钛表面含辛伐他汀钙磷涂层的成骨活性研究
Investigation of Osteogenic Effects about Ca-P Biomimetic Coatings Including Simvastatin on Pure Ti Surfaces
【作者】 张丽娟；
【导师】 赵士芳；
【作者基本信息】 浙江大学 ， 口腔种植学， 2010， 硕士
【摘要】 目的：创建一种含药仿生涂层,评估其的成骨效用。材料与方法：本实验中,我们将不同浓度的辛伐他汀加入到放生溶液中,通过仿生法使药物与钙磷离子共沉积于钛片表面。应用扫描电子显微镜(SEM)、傅立叶红外光谱(FTIR)和X射线衍射仪(XRD)观察表面的形貌和结构。体外试验中,我们应用碱性磷酸酶(ALP),检测涂层对MC3T3-E1细胞的影响。结果：通过扫描电子显微镜(SEM)、傅立叶红外光谱(FTIR)和X射线衍射仪(XRD),我们观察到低剂量的药物并没有改变钙磷涂层的宏观结构,而是掺入到晶体的结构中。在含药涂层表面培养MC3T3-E1细胞4天、7天和14天后,检测碱性磷酸酶的含量,发现涂层内的辛伐他汀能够释放出来,刺激MC3T3-E1细胞的分化,促进细胞功能的发挥,且1uM组作用效果最为明显。结论：我们可以认为仿生法是携带药物极为有效的方法,它可以不改变药物的性质的同时将其混入晶体内,并能够将药物缓慢释放出来,发挥其应有的作用。另外,我们可以得出,低剂量的药物较高剂量更为安全,更能促进成骨细胞的功能。
【Abstract】 Materials and Methods:In today’s studies,we prepare simvastatin solustion with different concentration that be add into simulated body fluids which can be co-deposited onto the surface of titanium plates. By scanning electron microscope (SEM), X-ray diffraction apparatus(XRD) and fourier transform infrared spectroscopy(FTIR),we can observe the micostructure.In vitro,we can gain its influence on MEC3T3-E1 cells by detecting the alkaline phosphatase(ALP) activity.Results:It is observed that in low-dose group,drugs had not only influenced the macrostructure of Ca-P coatings, but also became one part of Ca-P crystals.In vitro, MC3T3-E1 cells were inoculated onto the surface of biomimetic coatings in4,7 and 14 days, we found drugs could be released from the coatings and induce MC3T3-E1 cells differentiation in all groups especially in 1uM groups by alkaline phosphatase(ALP) detection.Conclusion:We can conclude that biomimetic means is the most effective way to incorporate drugs into surfaces of implants without influencing drug’s characteristics and make them released sustainedly to produce a marked effect.Meanwhile,it is found that low-dose drugs are more effective and safer than high-dose ones.