【作者】 陈氏红；

【导师】 杨斌；

【作者基本信息】 广西医科大学 ， 药理学， 2010， 硕士

【摘要】 目的:观察针刺对阿霉素(adriamycin ADM)所致大鼠心肌损伤和骨髓抑制的保护作用。方法: 45只SD大鼠随机分为4组:空白对照组10只,ADM模型组15只,针刺预防组10只,针刺治疗组10只。空白对照组大鼠腹腔注射生理盐水模型对照组,针刺预防组,针刺治疗组大鼠隔天腹腔注射阿霉素1.5mg·kg-1,共6次,11天。针刺预防组在造模期间同时给予针刺治疗直到实验结束;针刺治疗组在造模结束后给予针刺7天。第19天测定大鼠心电图,计算死亡率,心脏系数;检测心肌酶:乳酸脱氢酶(LDH)、谷草转氨酶(AST)、肌酸激酶(CK )的活性,检测肌酸激酶同工酶( CK-MB)值;观测心肌病理的改变。计算白细胞计数(WBC)、红细胞计数(RBC)、血红蛋白测定(Hb)、血小板计数(PLT),骨髓有核细胞计数(BMNC)等指标。结果:1对心肌损伤的影响:1.1与ADM模型组比较,针刺预防组和针刺治疗组均可显著改善ADM所致的心功能损伤,减少死亡率,减少CK、CK-MB、LDH、AST的释放(P<0.01或P<0.05)。1.2光镜下观察,ADM组心肌纤维排列紊乱、肿胀、部分断裂,甚至出现片状坏死,周围有炎性细胞浸润,红细胞漏出明显。针刺预防组,针刺治疗组的损伤明显减轻。2对骨髓抑制的影响:与ADM模型组比较,针刺预防组大鼠外周血中WBC,PLT及BMNC增高(P<0.01或P<0.05);针刺治疗组的PLT,BMNC水平明显升高具有显著性差异(P<0.05)。其他检测指标与ADM模型组相比有上升趋势,但无统计学意义。结论:1针刺对ADM大鼠的心肌功能与结构均有明显的保护作用。针刺能减少大鼠死亡率,减少心肌酶水平,减轻心肌病理改变。2针刺可改善外周血象,促进骨髓细胞增殖,对经ADM损伤后大鼠的骨髓造血系统有较好的保护作用。更多还原

【Abstract】 Objective: To observe the protective effect of electroacupuncture (EA) on myocardium injury and bone marrow suppression induced by adriamycin (ADM) in rats.Methods: 45 SD rats were equally divided into 4 groups:normal control group (NC) with 10 rats, ADM model group (ADM) with 15 rats, electroacupuncture prevention group (EAP) with 10 rats, electroacupuncture treatment group (EAT) with 10 rats. The normal control group was given normal saline by intraperitoneal injection, while other three groups were given ADM at a single dose of 1.5mg·kg-1 by intraperitoneal injection every other day, six times for eleven days.On the nineteenth day, the indexes, such as electrocardiogram (ECG), death rate, heart coefficient and the myocardial enzyme :lactate dehydrogenase (LDH), aspartate aminotransferase (AST), creatine kinase (CK) and creatine kinase isoenzyme MB (CK-MB) were detected. The pathological lesion of myocardium was observed. White blood cell count (WBC), red blood cell count (RBC), hemoglobin measurement (Hb), platelet count (PLT), bone marrow nuclear cell count (BMNC) were detected in rats. Results:1 The effects on the myocardium injury:1.1 Electroacupuncture prevention group and electroacupuncture treatment group could recover cardiac function, reduced the death rate and reduced the release of CK, CK-MB, LDH, AST (P<0.01 or P<0.05) when compared with the ADM model group.1.2 Myocardial fibers were in disorder condition under optical microscope in ADM model group, in which cellular edema, break or necrosis, infiltration of inflammatory cells, or obvious leakage of red blood cells could be observed. The above injuries could be significantly alleviated in electroacupuncture prevention group and electroacupuncture treatment group.2 The effects on bone marrow suppression:The electroacupuncture prevention group could increased significantly the contents of WBC, PLT and BMNC (P<0.01 or P<0.05); electroacupuncture treatment group could increased significantly the contents of PLT, BMNC (P<0.05) when compared with the ADM model group.Conclusion:1 EA can protect the heart function and myocardial cellular structure against ADM. EA can reduce the death rate, reduce the release of myocardial enzyme and relieve the pathological lesion of myocardium.2 EA has a better protective effect on bone marrow suppression induced by ADM in rats, which can improve the peripheral blood, and promote the proliferation of bone marrow cells.更多还原