【作者】 季雪梅；

【导师】 李晓燕； 陈娅蓉； 缪应雷；

【作者基本信息】 昆明医学院 ， 内科学， 2010， 硕士

【摘要】 目的:检测细胞型Fas相关死亡域蛋白样白细胞介素-1β转换酶抑制蛋白(cFLIP)、半胱氨酸天冬氨酸特异性蛋白酶-8(caspase-8)mRNA在结直肠癌组织中的表达,分析两种基因与结直肠癌发生发展的关系,探讨其在结直肠癌的诊断和治疗中的作用和意义。方法:采用实时荧光定量聚合酶链(SYBR Green I QRT-PCR)技术检测cFLIP、caspase-8mRNA在32例结直肠癌组织、10例癌旁组织及10例正常粘膜中的表达。结果:cFLIP、caspase-8在癌组织中的相对表量分别为(1.913±0.845)、(1.856±0.810),两者在癌旁组织中的相对表量分别为(1.521±0.447)、(1.597±0.578),两者在癌、癌旁组织中的表达均高于癌旁正常组织(p＜0.05)。其中cFLIP在癌组织中的表达与临床病理参数(性别、年龄、分化程度、肿瘤直径、有无淋巴结转移、有无远处转移、浸润程度,分布部位)无关(p＞0.05),caspase-8在有淋巴结转移组中的相对表达量(2.009±0.765)高于无淋巴结转移组(1.315±0.770),其与其它的临床病理参数(性别、年龄、分化程度、肿瘤直径、有无远处转移、浸润程度、分布部位)无关(p＞0.05).结论:cFLIP、caspase-8高表达在结直肠癌的发生发展中起重要作用,两者可能无线性关系。cFLIP表达与肿瘤的临床病理参数无关,caspase-8在有淋巴结转移组的相对表达量高于无淋巴结转移组,提示其与结直肠癌的侵袭转移及预后密切相关。因此认为cFLIP、caspase-8与肿瘤的发生发展、预后有关,有望成为肿瘤诊断和治疗的新靶标。更多还原

【Abstract】 Objective: To explore the effect and significance of Fas- associated death domain-like interleukin-1 converting enzyme (FLICE)- like inhibitory protein (cFLIP) and cystine asparate specific protease-8 (caspase-8) in diagnosing and treating with colorectal neoplasms, we investigated the expression of in colorectal neoplasms at mRNA level, and analyzed relationship between the occurrence and development of colorectal neoplasms with them,Methods: The expression of cFLIP、caspase-8 was detected in colorectal neoplasms, nearby tissues and matched normal tissues, at mRNA level, was detected by SYBR Green I quantitatie realtime polymerase chain reaction technique.Result: The relative expression amount of cFLIP, caspase-8 in colorectal neoplasms tissues were (1.913±0.845) , (1.856±0.810) , respectively; The relative expression amount of cFLIP, caspase-8 in nearby tissues was (1.521±0.447), (1.597±0.578) , respectively. The expression of them in neoplasms and nearby tissues was higher than that in normal tissues(p < 0.05). No relationship could be exist in cFLIP and clinicopathological parameters (including sex, age, differentiation degrees, size, lymph node metastasis, distant metastasis, invasion, distribution) (P>0.05), while caspase-8 in group with lymph node metastasis seemed much higher than that in group without lymph node metastasis (P<0.05), and there were no statistical differencese among groups devided by other different clinicopathological parameters (P>0.05).Conclusion: The overexpresstion of cFLIP and caspase-8 played a great role in the occurrence and development of colorectal neoplasms, but there might be no linear relationship between them. We couldn’t observe cFLIP correlated with clinicopathological parameters, while caspase-8 in group with lymph node metastasis was higher than that in group without lymph node metastasis. It suggested that caspase-8 had close relationship with the malignant degree and prognosis of colorectal neoplasms. To sum up, we concluded cFLIP and caspase-8 had close relationship with the occurrence, development and prognosis of colorectal neoplasms, and them could be new targets of the diagnosis and treatment of colorectal tumors in the future, hopefully.更多还原