【作者】 王丛笑；

【导师】 周军；

【作者基本信息】 首都体育学院 ， 运动人体科学， 2010， 硕士

【摘要】 目的：针对目前对运动性疲劳恢复的研究热点,通过对国内外有关中药抗运动性疲劳和神经内分泌免疫网络(NEI)的文献资料的查阅,初步了解它们之间的关系。本研究通过大鼠运动性疲劳模型,观察具有疏肝理气、益气养阴、养血.安神功效的中药复方理气调补汤对运动疲劳训练大鼠NEI的影响,以揭示其在延缓疲劳、加速疲劳恢复及提高机体运动能力方面的作用机理。方法：健康雄性SD大鼠30只,随机分为正常对照组8只、模型组11只、中药组11只。中药组每天定时定量中药灌胃1mL/100g体重,对照组、模型组灌胃相同剂量的生理盐水。除对照组外,其余大鼠进行6周大强度游泳运动训练,6周末进行负重大鼠自身体重5%的力竭训练。力竭游泳实验24 h后麻醉,腹主动脉取血5mL,分离血清用ELISA法测定大鼠血清β内啡肽(β-EP)、皮质酮(C)、睾酮(T)、黄体生成素(LH)、白细胞介素2(IL-2)。同时,取血后分离下丘脑,切碎匀浆,离心取上清液用ELISA法测下丘脑β-EP含量。结果：(1)大鼠力竭时间中药组与模型组相比显著延长(P<0.01)。(2)比较对照组和模型组,长期大强度游泳训练后,大鼠血清T含量下降(P<0.01),血清C升高(P<0.01),T/C比值显著降低(P<0.01),血清IL-2含量下降(P<0.01)造成大鼠机体运动性疲劳状态。与模型组相比,中药组大鼠血清T含量显著升高(P<0.05),血清C显著降低(P<0.01),T/C比值显著升高(P<0.01)。IL-2含量显著升高(P<0.05)。(3)模型组大鼠血清β-EP浓度显著高于对照组和中药组(P<0.05)。下丘脑β-EP含量模型组显著高于对照组(P<0.05),中药组下丘脑β-EP含量较模型组有所降低,但下降不显著。结论：(1)理气调补汤可以延长大鼠的力竭游泳时间,使T、C、T/C比值趋于正常,能够调节血清和下丘脑β-EP的分泌,维持下丘脑-垂体-肾上腺轴和下丘脑-垂体-性腺轴的正常功能。(2)理气调补汤能够提高IL-2的活性,可能参与了其调节机体免疫水平的作用。(3)理气调补汤可以通过调节NEI的功能,维持系统的稳定,达到预防和消除运动性疲劳的作用。更多还原

【Abstract】 Objective:For the current research focus on Exercise-induced fatigue recovery, Through search information at home and abroad of Chinese medicine for anti-exercise-induced fatigue and NEI, Initial understanding of the relationship between them. This experiment in rat model of exercise-induced fatigue, Observe the effects of Liqi Tiaobu Tang decoction on NEI of the rat of fatigue induced by exercise. Reveal the mechanism of the decoction in the field of delaying fatigue, speeding recovery and improving physical function.Methods:30 male SD rats were randomly divided into 3 groups:control group 8, model group 11, medicine group 11. Medicine group ig the certain dosage Liqi Tiaobu Tang every day. Control group and model group ig the certain dosage Saline every day. In addition to control group, the rest rats trained for 6 weeks with swimming training method. By the end of the training, load 5% of their body weight. All rats were killed on 24 hours after last training, Taking blood 5mL from the abdominal aorta. Separate serum and Test the content ofβ-EP、C、T、LH and IL-2. Then separate hypothalamus, Chopped and Homogenate, centrifugal and get the supernatant. Using ELISA test the content of hypothalamusβ-EP.Results:(1) The time of the medicine group rats swimming to exhaustion was significantly prolonged compared with model group(P<0.01).(2) Compared with the control group, levels of serum T and IL-2 in the model group were markedly decreased(P<0.01), levels of serum C were markedly increased(P<0.01), T/C were markedly decreased(P<0.01). Rats fatigue caused by overtraining.Compared with the model group, content of serum T and IL-2 in the medicine group were significantly enhanced(P<0.05). Content of serum C were markedly decreased(P<0.01), T/C were markedly increased (P<0.01).(3) Compared with the control group and the medicine group, content of serumβ-EP in the model group were markedly increased(P<0.05). The content of hypothalamusβ-EP in the model group were markedly increased compared to the control group (P<0.05). Compared with the model group, content of hypothalamusβ-EP in the medicine group were slightly decreased, but the change isn’t significant.Conclusion:(1) Liqi Tiaobu Tang can prolong the exhaustive swimming time. It can regulate the secretion of serum T、C、β-EP and hypothalamusβ-EP. Keep balance of the hypothalamic-pituitary-glandular axis.(2) Liqi Tiaobu Tang can enhance the activity of IL-2, play a role in regulating immune function. (3) Though regulating the function of nerve-endocrine-immune network, Liqi Tiaobu Tang can maintain system stability. It can prevent the exercise fatigue.更多还原