【作者】 施莉莉；

【导师】 刘希琴；

【作者基本信息】 南京航空航天大学 ， 核技术及应用， 2009， 硕士

【摘要】 放射神经生物学是辐射生物学的一个新的分支,越来越受到国内外辐射生物学研究者的关注。神经、内分泌、免疫诸系统之间存在着相互作用。中枢神经系统与免疫系统之间存在两条途径,其一是下丘脑-垂体-肾上腺轴,另一条是通过自主神经对免疫系统进行调节。目前国内放射神经生物学主要涉及中枢神经在受到电离辐射刺激后的形态学和功能学的改变。国外主要集中在电离辐射作用后生物体的功能学改变。免疫细胞是辐射最敏感的细胞群体,细胞表面表达多种受体,其中与神经递质结合的受体则是神经系统调控免疫系统的物质基础。神经系统对受辐射机体免疫系统的影响在放射损伤和放射治疗中具有重要的理论和临床价值,但迄今为止有关这方面的研究报道还很缺乏。本试验用6-OHDA阻断外周交感神经传导,通过检测小鼠外周血中淋巴细胞及其亚群细胞数量、IL-2、NO酶和NO合酶的含量、胸腺指数、脾指数等指标的变化来研究交感神经阻断对受辐照小鼠免疫系统的影响,探讨交感神经阻断对受辐照小鼠免疫系统的作用。结果显示(1)随着辐照剂量的增加,辐照组小鼠淋巴细胞及其各亚群受损加剧;阻断组小鼠淋巴细胞及其各亚群的变化趋势同辐照组,中、高剂量组下降更加明显。(2)辐照组小鼠体重、脾指数、NOS活力、NO含量等在0.2Gy处有最大值,以后随剂量加大,从0.5Gy到3Gy,数值逐渐减少;而胸腺指数随剂量增加而递减。(3)3Gy组小鼠IL-2的含量为3Gy阻断神经组>空白组>3Gy非阻断神经组。实验表明去甲肾上腺素对受辐照小鼠的免疫系统的恢复有积极的作用。由于交感神经阻断进一步增强了受辐照机体的免疫抑制作用,在放射治疗过程中应避免损伤交感神经。更多还原

【Abstract】 Radioneurobiology is a new subject of radiobiology. More and more people in and abroad are paying attention to radiobiology. There are interactions among nerve, endocrine system and immune system. There are two ways between central nervous system and immune system, one is hypothalamic-pituitary-adrenal axis, the other one is through autonomic system. This subject involves the morphological and functional changes of central nerve receiving irradiation. There are much more studies in this area abroad, mainly focusing on the functional changes in biotics after irradiation. Immune cell is the most sensible cell group. There are many receptors expressed on the immune cell. The receptor bonded with neural transmitter is the basic of nervous system regulating the immune system. So the study of the nervos system and its influence in immune system has theoretical and clinical value in irradiation curation. But the study in this area is very rare. We used 6-OHDA to block the peripheral transduction of sympathectical nerve. Then we watched the effect of sympathectomy on the immune system by test the changes in quantity of immune cells and its subgroups, IL-2, NO and NOS in the peripheral blood, thymus index and spleen index of irradiated mice.The results show (1) the more the dosimetry of irradiation is, the more damage causes in immune cells and its groups; the irradiate effect of sympathectomized group is the same as that of the unsympathectomized group, and the mice that received irradiation of mediate and high dose of had much smaller number of immune cells. (2) weight, thymus index, spleen index, NOS, NO have the highest quatity after receiving 0.2Gy irradiation, and declined with the increasing dose. (3) the quantity of IL -2 in the peripheral blood is: 3Gy+S>control>3Gy(S means sympathectomy).The conclusion shows that NE has positive function for the recovery of mice. The sympathetic nerve should be watched when the patient are receiving irradiation therapy.更多还原