【作者】 谢晓芳；

【导师】 彭成；

【作者基本信息】 成都中医药大学 ， 方剂学， 2009， 硕士

【摘要】 目的通过对补经胶囊主要药效和辅助药效学研究,探讨补经胶囊补肾养血调经的生物学效应及治疗月经不调（月经后期、量少）的可能作用机制。方法选用大鼠去势肾虚模型和小鼠氢化可的松模型研究补经胶囊补肾作用,小鼠失血性血虚和溶血性血虚模型研究补经胶囊养血作用,大鼠雄激素致无排卵模型（ASR）、大鼠离体子宫模型、家兔在体子宫模型研究补经胶囊调经作用。氢化可的松肾虚小鼠在给药9天后测定肛温、负重游泳时间,以及子宫、卵巢、胸腺、脾脏的脏器系数。两种血虚动物模型给药7天后用BC3000血球仪测定外周血红细胞计数（RBC）、血红蛋白（HGB）含量和网织红细胞比率（Ret）。放免法测定去势大鼠给药5周和ASR大鼠给药8周后血清FSH、LH、E₂和P含量,HE染色观察两模型大鼠子宫、乳腺、阴道等激素靶器官的病理组织学变化。用BL-420生物机能系统记录大鼠离体子宫和家兔在体子宫收缩曲线。结果补经胶囊能增加去势肾虚大鼠子宫湿重和脏器系数,升高模型大鼠血清E2水平,病理学检查显示补经胶囊能改善模型大鼠子宫内膜及乳腺的萎缩现象；能显著升高氢化可的松肾虚模型小鼠的肛温,延长负重游泳时间,提高胸腺和脾脏指数。补经胶囊能升高失血性血虚小鼠RBC和HGB含量,降低Ret,升高乙酰苯肼致血虚小鼠RBC含量。补经胶囊可减轻ASR模型体重,增加模型大鼠子宫和卵巢脏器系数,并能升高血FSH、LH水平,病理学检查显示可改善模型大鼠卵巢、子宫、乳腺的萎缩现象；能增加大鼠离体子宫的收缩频率、收缩强度和子宫活动力,但对催产素引起的子宫痉挛无明显作用；能增强家兔在体子宫的收缩强度和活动力。结论补经胶囊具有补肾养血调经作用,可用于治疗肾虚血亏型月经不调（月经后期、量少）。推测调经作用机制可能与改善下丘脑-垂体-卵巢轴（H-P-0）内分泌功能及雌激素样作用有关。更多还原

【Abstract】 Objective:To investigate the biological effects of strengthening kidney, nourishing blood and regulating menses of Bujing Capsule and its potential mechanism for menstruation disorders by major and minor pharmacodynamic experiments.Methods:Female Castrated rats and hydrocortisone-induced kidney-yang deficiency mice were used as kidney-dificiency animal models for the study of strengthening kidney. Bleeding-induced anaemia mice and acetylphenyhydrazine-induced anaemia mice were used to investigate the nourishing blood action of Bujing capsule. Androgen-induced sterile rats (ASR), in vitro uterine muscle strips of rats, nd in vivo uterine muslce of rabbits were applied to investigate menses regulating effect of Bujing capsule, as well as potential mechanism.Rrectal temperatures, weight loading swimming time, and organ coefficients of thymus, spleen, liver, uterus, and ovary were measured for hydrocortisone-induced kidney-yang deficiency mice after 9 days’ drug administration. After treating with Bujing capsule for 7 days, the red blood cell count (RBC), hemoglobin (HGB) and reticulocytes Ret in peripheral blood of anaemia mice models were assayed by BC3000-type blood analyzer. Serum levels measures of Estradiol (E2), Progesterone (P) of castrated rats after drug administration for 5 week and E2, P, follicle-stimulating hormone (FSH), luteinizing hormone (LH) of ASR after drug administration for 8 weeks were done with radio-immunity testing. Tissues of uteruses, mammary glands, vaginas, pituitary glands of both two models and ovaries from ASR were fixed by 10% formaldehyde for histopathologic examination with HE staining. Uterine muscle contraction activities of in vitro uterine muscle strips from rats and in vivo uteruses from rabbits were reccorded with BL-420 biological functional system.Results:For castrated rats, Bujing capsule increased uterine weight and organ coefficient significantly, as well as serum level of E2. The atrophy of endometrium and mammary gland of castrated rats were improved for some extent. For hydrocortisone-induced kidney-yang deficiency mice, Bujing capsule could increase the low rectal temperature, prolong weight loading swimming time, and arised ovary and uterine coefficients. Bujing capsule successfully improved contents of RBC and HGB and decreased Ret for Bleeding-induced anaemia mice.It also significantly elevated RBC content for acetylphenyhydrazine-induced anaemia mice.For ASR, Bujing capsule markably showed effcts of lowering body weight, increaing ovary and uterine coefficients, revising serum contents of FSH and LH, and improving the atrophy of ovaries, uteruses and mammary glands. Also, Bujing capsule significantly increased frequency, magnitude, and motoricity of in vitro uterine muscle contraction of rats,but had no apparent effect on oxytocin-induced uterine muscle spasm. Bujing capusle also showed significantly effects on strengthening magnitude and motoricity of in vivo uterine muscle contraction of rabbits.Conclusions:Bujing capsule has biological activities of strengthening kidney, nourishing blood, and regulating menses (including delayed menorrhea and less menstrual flow) and is appropriate for menstruation disorders of kidney and blood deficiency pattern. The potential mechanism of menses regulation may contributes to progressing endocrine function of hypothalamus-pituitary-ovary axis (H-P-O) and its estrogen-liking effects.更多还原