【作者】 徐翔；

【导师】 董强； 任惠民； 魏文石； 王亮；

【作者基本信息】 复旦大学 ， 神经内科， 2009， 硕士

【摘要】 目的:探讨Y迷宫训练后大鼠脑内MMP-9表达及其在海马依赖的学习记忆中的作用。方法:3月龄雄性SD大鼠,随机分成对照组(CON,n=12)和Y迷宫训练组(T,n=60),后者又根据训练天数和处死时间分成5组。计算大鼠在Y迷宫训练中的连续正确反应次数,观察其学习能力;用Western blot检测各组动物不同脑区MMP-9前体(proMMP-9)和活性形式(actMMP-9)的蛋白表达,用免疫组化学和RT-PCR分别检测海马区MMP-9蛋白和mRNA的表达。探讨MMP-9表达与其学习能力的相关性。结果:1)Y迷宫训练早期,大鼠海马区出现actMMP-9蛋白水平一过性增加,同时其MMP-9mRNA表达也相应升高;而额叶区未出现类似变化。2)Y迷宫第一天训练后4h大鼠海马区actMMP-9表达水平与其第一天训练成绩(平均连续正确反应次数)呈正相关(p＜0.05)。结论:actMMP-9可能参与了大鼠Y迷宫的学习过程。更多还原

【Abstract】 Objective: To study matrix metalloproteinase -9 (MMP-9)expression in the rat brain after Y maze training and its role in the hippocampal-dependent learning and memory. Methods: Male Sprague-Dawley rats(3 months old) were assigned randomly to control group (CON, n=12) and training group (T, n=60). The training group was also divided into five groups. Rats were evaluated for their learning ability in Y maze by measuring the number of continuous correct responses in finding the safe place with repeated trials. Then two forms of MMP-9(proMMP-9 and actMMP-9) protein were detected and quantitated from different rat lobs and in different time by Western blot. The expression of MMP-9 and MMP-9mRNA were detected by immumohistochemistry and RT-PCR, respectively. Furthermore, the correlation between the learning ability of rats in Y maze training and the levels of MMP-9 in hippocampus was evaluated. Results: 1) It was observed that hippocampal actMMP-9 increased transiently in the early time during Y maze training as assessed by Western blotting and mRNA analysis. But the same appearances were not found in prefrontal cortex of all the groups. 2) Four hours after the first day training, levels of actMMP-9 in the hippocampus of rats were significantly correlated with their numbers of the continuous correct responses. Conclusion: ActMMP-9 may involved in the hippocampal-dependent learning during Y maze training.更多还原