【作者】 周强；

【导师】 马保华；

【作者基本信息】 西北农林科技大学 ， 临床兽医学， 2009， 硕士

【摘要】 以小鼠胚胎体外发育和体内植入为试验模型,将小鼠早期胚胎暴露于含不同质量浓度Aroclor 1254的胚胎培养液中,进行Aroclor 1254对小鼠胚胎的体外发育毒性和体内植入毒性试验,并进行体内发育胚胎的植入毒性试验,探讨Aroclor 1254对小鼠胚胎发育和植入的毒性作用及其影响规律。1.探讨Aroclor 1254对小鼠不同时期胚胎体外发育和移植于受体鼠后胚胎植入的影响。采集不同发育时期小鼠胚胎(2-细胞、4-细胞和8-细胞胚胎),在体外培养条件下,以不同持续时间暴露于不同试验溶液(分别含0.05, 0.25, 1.25μg/mL Aroclor 1254的3个Aroclor 1254毒性试验溶液、溶剂对照溶液、空白对照溶液),记录各时期胚胎发育情况。当胚胎在上述各溶液中体外发育至囊胚阶段后,移植到受体鼠子宫角。在受体鼠见栓后第8天(dpc8.5)上午,尾静脉注射台盼兰,之后处死小鼠,观察、记录胚胎植入位点数。试验结果显示,溶剂对照组与空白对照组的胚胎体外发育和胚胎植入情况没有统计学差异;小鼠2-细胞和4-细胞胚胎暴露于0.25μg/mL Aroclor 1254毒性溶液12 h后,胚胎发育会受到明显影响,并影响胚胎移植后的植入(P<0.05);小鼠8-细胞胚胎暴露于0.25μg/mL Aroclor 1254毒性试验组24 h后,会影响其体外发育,并影响胚胎移植后的植入(P<0.05)。随着毒性试验溶液中Aroclor 1254质量浓度的提高和暴露时间的增加,Aroclor 1254对小鼠胚胎的毒性影响逐渐明显,对小鼠胚胎体外发育和胚胎移植后的植入,毒性影响均呈现一定的时间-剂量-效应关系。2.探讨Aroclor 1254对小鼠体内发育胚胎植入的影响。选取自然发情配种的小鼠为试验鼠,于配种后第4天(dpc 4.5),子宫角注射不同试验溶液(分别含0.25, 1.25, 6.25μg/mL Aroclor 1254的3个Aroclor 1254毒性试验溶液、溶剂对照溶液、空白对照溶液)。配种后第8天(dpc 8.5),尾静脉注射台盼兰,之后处死小鼠,观察、记录胚胎植入位点数。试验结果表明,当Aroclor 1254毒性溶液中Aroclor 1254的质量浓度达到到6.25μg/mL时,Aroclor 1254对胚胎植入呈现明显的毒性影响(P<0.01);当Aroclor 1254毒性试验组的质量浓度为1.25μg/mL,试验侧子宫角胚胎植入位点数与溶剂对照组无统计学差异,但平均植入位点数有下降趋势,说明该浓度已经开始对胚胎植入产生一定影响。结论认为,当体内发育胚胎暴露于一定浓度的Aroclor 1254时,会影响胚胎正常植入,Aroclor 1254对小鼠胚胎植入的影响呈现一定的剂量-毒性效应关系。更多还原

【Abstract】 The study was based on the models of mouse embryo development in vitro and implantation in vivo, and investigated the effect of different concentration of Aroclor 1254 on mouse embryo development in vitro and implantation in vivo.1. The experiment investigated the toxic effect of Aroclor 1254 on embryo development in vitro and embryo implantation after embryo transfer. Different stages of mouse embryo(2 cell, 4 cell, 8 cell) were collected and exposed to the different dosages toxicity solution of Aroclor 1254 (0.05, 0.25, 1.25μg/mL) for different time, comparing to solvent control group(containing ethanol)and blank control group. When the mouse embryo developed into blastocysts, they were transferred into the horns of uterus in recipient mice. In the eighth morning of mouse mating (dpc8.5), trypan blue was injected into vena caudalis for detecting implantation site. The results indicated that there was no significant difference between solvent control group and blank culture group in embryo development and embryo implantation. The mouse embryos of 2 cell and 4 cell were exposed to Aroclor 1254 of 0.25μg/mL for 12 h, then mouse embryo development in vitro and implantation in vivo were significantly restrained (P<0.05). The mouse embryos of 8 cell were exposed to Aroclor 1254 of 0.25μg/mL for 24 h, then mouse embryo development in vitro and implantation in vivo were significantly restrained (P<0.05). With the increasing of the dosages and the exposure time of Aroclor 1254, the toxical effect to mouse embryos acted more fiercely. It indicated that the toxical effect of Aroclor 1254 on the mouse embryo according to the time-dose.2. The aim of this experiment is to investigate the effect of Aroclor 1254 on mouse embryo implantation in vivo. The female mouses of natural empathema were selected as test mouse. In the fourth day after mating (dpc4.5), the different toxicity solution (0.25, 1.25, 6.25μg/mL) was injected into horn of uterus. In the eighth morning afer mouse mating (dpc8.5), trypan blue was injected into vena caudalis for detecting implantation site. The results indicated that Aroclor 1254 of 6.25μg/mL significantly restrained embryo implantation (P<0.01). Aroclor 1254 of 1.25μg/mL also restrained embryo implantation, but there was no significant difference between Aroclor 1254 of 1.25μg/mL and solvent control group in embryo implantation sites. It revealed that Aroclor 1254 of 1.25μg/mL had begun to affect embryo implantation. The conclusion was that Aroclor 1254 restrained embryo implantation evidently according to the dose-toxicity.更多还原