节点文献
人MGC5306基因原核、真核表达载体的构建及亚细胞结构的定位
Prokaryotic/Eukaryotic Expression, Preparation of Human MGC5306 Gene and the Subcellular Localization of MGC5306
【作者】 王劼；
【导师】 雷鸣；
【作者基本信息】 西北农林科技大学 ， 生物化学与分子生物学， 2008， 硕士
【摘要】 MGC5306基因作为一个新发现的基因,目前国内外对其研究的相关报道还非常少。有实验证据表明MGC5306参与卵巢癌的发生。MGC5306的功能及其作用机理现在还不完全清楚。我们利用基因重组技术构建了MGC5306的原核表达体系,并成功表达了MGC5306(98-204AA)。我们构建了pDsRed-MGC5306,并将其转染至Hela细胞,观察其亚细胞结构的分布及细胞核内的定位。将DsRed-MGC5306和GFP-SUMO1共转染至Hela细胞,通过激光共聚焦发现两者共定位于细胞核内。
【Abstract】 As a newly discovered gene, domestic and foreign research reports of MGC5306 are also less now. There is experimental evidence that the MGC5306 involved in the occurrence of ovarian cancer. MGC5306 functions and its mechanism is not entirely clear. We use gene recombination technology to build the prokaryotic MGC5306 expression system, and we have successfully expressed MGC5306(98-204AA).We build eukaryotic expression DsRed- MGC5306, and transfected it into Hela cell to observed sub-cellular distribution and nuclear localization of protein. Eukaryotic expression vectors pDsRed-C1-MGC5306 and pEGFP-N1- SUMO1 were transfected into Hela cell, we found MGC5306 and SUMO1 co-localize in the nucleus by CLSM(Confoca Laser Scanning Microscopel).
【Key words】 MGC5306； prokaryotic/ eukaryotic expression； fusion protein； sub cellular structure； localization；