【作者】 邢涛；

【导师】 孙汉文；

【作者基本信息】 河北大学 ， 分析化学， 2007， 硕士

【摘要】 喹诺酮类药物是一类全合成抗感染药物,已经广泛应用于医学和兽医学中,该类药物在被大量使用的同时带来了健康安全相关的问题。因此对喹诺酮类药物的分析研究已经引起人们的重视。发展可靠、灵敏和实用的分析技术无疑是检测喹诺酮类药物质量、检测和控制兽药残留、保证食用者安全和避免国际间有关贸易争端的重要前提。电化学分析法具有灵敏度高,选择性好,响应时间短,方法简便,适用面广的特点；在测定过程中测定的是电信号,容易实现自动化、连续化和在线分析。近年来,修饰电极以其独特的性能正日益引起分析工作者的关注,它是在电极表面进行分子设计,造成某种微结构,赋予电极某种特定的化学和电化学性质,以便高选择性地进行所期望的反应,在提高选择性和灵敏度方面具有独特的优越性,特别是在生物样品和药物样品分析研究中得到迅速发展。本论文对玻碳电极的表面进行了处理,研究了喹诺酮类药物在处理电极表面的电化学性质,提高了电化学方法测定喹诺酮类药物的灵敏度,应用于药品的测定。全文分为如下四个部分：第1章：对电化学方法和喹诺酮类药物进行了简述,对电化学方法研究喹诺酮类药物的现状进行了综述,主要介绍了汞电极和玻碳电极对该类药物的研究情况。第2章：采用电化学方法对玻碳电极表面进行了处理,研究了诺氟沙星在该电极上的电化学特性。结果表明：电极过程为一个不可逆过程,主要由吸附控制；该方法提高了诺氟沙星测定的灵敏度,检出限为0.2μg/mL,线性范围为0.5-5.0μg/mL,可以用于诺氟沙星胶囊的测定。第3章：采用碳纳米管对玻碳电极表面进行了修饰,研究了依诺沙星在该电极上的电化学特性。结果表明：电极过程为一不可逆过程；该方法提高了依诺沙星测定的灵敏度,检出限为0.01μg/mL,线性范围为0.02-0.20μg/mL,可以用于依诺沙星胶囊的测定。第4章：采用聚合物薄膜对玻碳电极表面进行了修饰,研究了氧氟沙星在该电极上的电化学特性,结果表明：电极过程为一不可逆过程；该方法提高了氧氟沙星测定的灵敏度,检出限为0.02μg/mL,线性范围为0.1-1.0μg/mL,可以应用于氧氟沙星片剂的测定。更多还原

【Abstract】 Quinolone is a kind of synthetical antibiotics and has been widely used in human and veterinary medicine. It has brought some problems about health and safety for its wide use. So the analysis of quinolones attracts people’s attentions. Developing reliable, sensitive and practical methods is the precondition of pharmaceutical quality control and residue analysis, and is necessary to assure the safety of food and to avoid international trade issue.Electrochemical analysis is characterized by high sensitivity, high selectivity, fast response and wide use. Because what are detected are electricity signals, it is easy to realize automation, series and on-line analysis. Especially nowadays, the appearance of modified electrode has attracted analysts’ attentions with special characteristics. Modified electrode is to design on the surface of the electrode, to make microstructure and to give electrode special chemical and electrochemical characteristics, in order to improve the sensitivity and selectivity. It has been improved in biological and pharmaceutical samples analysis.In this paper, the surface of glassy carbon electrode was modified in different ways and the electrochemical characteristics of quinolones on this electrode were studied. Modified electrode can improve the sensitivity and detection of quinolones, and can be applied on pharmaceutical analysis. It includes four chapters as follows:Chapter 1:The electrochemical methods and quinolone were reviewed, and the applications of electrochemical methods on quinolone analysis were introduced in details, especially the behaviors on mercury and glassy carbon electrode.Chapter 2:The glassy carbon electrode was treated by electrochemical method, and the electrochemical behaviors of norfloxacin on this electrode were studied. The electrochemical process was irreversible and fundamentally controlled by adsorption. The sensitivity of norfloxacin detection was improved, the detection limit was 0.2μg/mL and the linear range was 0.5-5.0μg/mL. This methond was used for determination of norfloxacin in capsule.Chapter 3:The glassy carbon electrode was modified with multi-wall carbonnanotubes functionalized with carboxylic group, and the electrochemical behaviors of enoxacin on this electrode were studied. The electrochemical process was irreversible. The sensitivity of enoxacin detection was improved, the detection limit was 0.01μg/mL and the linear range was 0.02-0.20μg/mL. This methond was used for determination of enoxacin in capsule. Chapter 4:The glassy carbon electrode was modified with poly (crystal violet) film, and the electrochemical behaviors of ofloxacin on this electrode were studied. The electrochemical process was irreversible. The sensitivity of ofloxacin detection was improved, the detection limit was 0.02μg/mL and the linear range was 0.1-1.0μg/mL. This methond was used for determination of ofloxacin in tablet.更多还原