【作者】 李虹娇；

【导师】 金英子；

【作者基本信息】 延边大学 ， 外科学， 2009， 硕士

【摘要】 目的:研究甘油果糖注射液对宫内缺氧缺血胎鼠脑组织自由基和细胞凋亡的影响,探讨甘油果糖对宫内窘迫胎鼠脑组织的保护作用。方法:WiStay雌性大白鼠,于妊娠第19d通过无损伤动脉钳钳夹双侧子宫、卵巢血管20min建立缺血缺氧宮内窘迫模型。孕鼠随机分为假手术组(A组),缺血缺氧组(B组),甘油果糖治疗组(3.5ml/100g,腹腔注射):甘油果糖HI前30min治疗组(C组)和HI后立即治疗组(D组)。此后实验用母鼠均关腹放回原饲养环境中24h后,立即剖宮取胎,确定胎鼠是否存活,在每组存活胎鼠中随机抽取10只断头取脑,以比色法测定脑组织丙二醛(MDA)含量、TUNEL法观察脑海马区神经元凋亡。结果:1、与缺血缺氧组比较,C组、D组胎鼠死亡率明显降低(P＜0.05,P＜0.05)。2、与缺血缺氧组比较,C组、D组脑组织MDA含量降低(P＜0.01,P＜0.05);与D组比较,C组脑组织MDA含量降低(P＜0.01)。3、与假手术组比较,缺血缺氧组脑海马神经元凋亡数目明显增加(P＜0.01)。C组、D组与缺血缺氧组比较,海马区凋亡细胞数目减少(P＜0.01,P＜0.05)。C组凋亡细胞数目多于D组(P＜0.01)。结论:1、钳夹孕鼠子宫、卵巢血管造成胎鼠缺氧缺血模型是模仿临床围生期胎儿窘迫较为理想的动物模型。2、实验证明甘油果糖能够通过胎盘屏障及血脑屏障,可能通过促进能量代谢途径对HIBD胎鼠起脑保护作用。甘油果糖是否可以通过直接清除自由基对HIBD起治疗作用需进一步研究探讨。3、实验表明缺氧缺血性脑损伤前用药比其后用药效果更佳.更多还原

【Abstract】 Objective: To study the effects of Glycerol Fructose on free radical andapoptosis of fetal rat brain sufered from intrauterine hypoxic-ischemicinjury,so as to explore the neuroprotective mechanisms of GlycerolFructose.Methods: Wistar female rats of gravidity were randomly divided into fourgoups : ham operation group ( group A ) , HI group(group B), GlycerolFructose-treated group (3.5ml/100g, intraperitoneal injection) : GlycerolFructose-treated ahead of HI(group C) , Glycerol Fructose-treatedimmediately after HI (group D). On the nineteenth day after gravidity , wemade the model of fetal rat distress by clamping the maternal uterine andovarian vessels in group B、C、D. The rats in group C were given GlycerolFructose 30 min ahead of HI, the rats in group D were given Glycerol Fructoseimmediately after HI. Group A was sham operated.The rats were made dead at24h after HI by cutting head and brain tissues were separately taken .Wejudged whether the fetal rats living or not , chose ten fetal rats randomlyeach group and measured the content of MDA in fetal brains and detectedthe number of hippocampus apoptosis .Results :1、The mortality rate of group C and D decreased significantly than thatof HI group (P<0. 05, P<0. 05).2、The content of brain tissue MDA in group C and D decreased significantlythan that of HI group (P< 0.01, P<0. 05). MDA in group D increasedsignificantly, higher than that of group C (P<0. 01).3、The number of hippocampus apoptosis in HI group increased significantlythan that of ham operation group (P<0.01) .The number of cerebralhippocampus apoptosis in group C and D decreased significantly than thatof HI group (P<0. 01, P<0. 05). Group C and D were significantly different (P<0. 01).Conclusions :1、It may be an ideal method to made the model of fetal rat distress byclamping the maternal uterine and ovarian vessels.2、Glycerol Fructose can pass placental barrier and hematoencephalicbarrier, Glycerol Fructose may protect brain by promoting energy metabolism.Whether Glycerol Fructose can protect brain by cleaning up free radicaldirectly, more research is needed.3、Preventive medication before HIBD is better than medicine interventionafter HIBD .更多还原