【作者】 付艳；

【导师】 江剑平；

【作者基本信息】 福建师范大学 ， 动物学， 2009， 硕士

【摘要】 感觉神经元特异性受体(sensory neuron-specific receptor,SNSR)SNSR的mRNA只独特地存在于脊髓背根神经节和三叉神经节的中小型神经元里,具有高度的组织特异性,该类神经元为伤害性信息传入的初级神经元,推测SNSR可能参与伤害性信息的调制。本实验应用SNSR受体的激动剂(BAM8-22,MSH)通过行为学、免疫组织化学等实验方法,研究SNSR受体在CFA慢性炎症痛大鼠脊髓背角和背根神经节痛觉调制中的作用。行为学实验结果表明:鞘内注射SNSR受体激动剂(BAM8-22,MSH)能剂量依赖地恢复CFA炎性大鼠热刺激缩脚反射潜伏期,降低热痛觉过敏,减轻足跖炎性红肿程度。免疫组织化学结果显示:鞘内注射BAM8-22能下调大鼠CFA炎症侧脊髓背角和背根神经节NOS,nNOS和CGRP阳性细胞表达量,从细胞水平阐明SNSR受体在炎症痛模型中痛觉传递和调制的作用。我们的研究证实SNSR受体参与了以DRG为代表的脊髓水平的痛觉调制,探讨SNSR受体在炎性痛中的作用及其与其它兴奋性神经递质之间的关系,有助于人们加深对疼痛机制的认识,也可为临床镇痛和新型镇痛药的开发提供依据。更多还原

【Abstract】 The mRNA of sensory neuron-specific receptor is uniquely located in the small diameter neurons in dorsal root ganglia(DRG) and trigeminal ganglia(TG).Because these neurons are the primary nociceptive sensory neuron,the physiological effect of SNSR may modulate the transfer of nociceptive signal.This study was conducted with behavioral and immunohistochemistry techniques application of SNSR ligands;Bovine adrenal medulla 8-22(BAM8-22) and Tyr6-γ2-MSH-6-12 to research the pain regulation of SNSR in the level of spinal cord dorsal horn and dorsal root ganglion.In behavioral experiment,i.t.(intrathecal injection) administration of SNSR agonists-BAM8-22 and Tyr6-γ2-MSH-6-12 recovery paw withdrawal latency by thermal stimulation,weaken the thermal hyperalgesia induced by i.pl.injection of complete Freund’s adjuvant(CFA),ease paw inflammation swell.The immunohistochemical result is that i.t.administration of BAM8-22 lead to decrease the positive neurons expression of NOS,nNOS and CGRP in spinal cord dorsal horn and DRG,which illustrate SNSR function in pain transference and regulation from cellular mechanisms in CFA-treated rats.The present study demonstrates that SNSR modulate the transfer of nociceptive signal.A better understanding of the mechanisms underlying the SNSR-induced the antihyperalgesic effects in chronic inflammation will be pivotal in the development of SNSR-targeted medication for the treatment of chronic pain.更多还原