【作者】 吴长阳；

【导师】 唐家龄；

【作者基本信息】 广州医学院 ， 妇科学， 2009， 硕士

【摘要】 目的:测量子宫内膜异位症(内异症EMs)患者腹腔液中碱性成纤维细胞生长因子(BFGF)、血管内皮生长因子(VEGF)和基质金属蛋白酶2(MMP-2)及其抑制物(TIMP-2)的含量改变,探讨这些物质与内异症的关系;分析内异症患者的瘦素受体(LEPR)Gln223Arg等位基因多态性,并探讨这种多态性与内异症患者腹腔微环境变化的相关性,进一步探讨子宫内膜异位症的发病机制。方法:采用ELISA方法检测约30例内异症妇女和30例非内异症对照组妇女腹腔液中MMP-2、TIMP-2、BFGF、VEGF的含量。采用PCR-RFLP法测定两组妇女瘦素受体基因的Gln223Arg等位基因型。结果:ELISA方法测定提示EMs组腹腔液中VEGF、MMP-2含量高于对照组,TIMP-2含量低于正常对照(P<0.05),差异有统计学意义。两组BFGF含量无明显差异(P>0.05)。两组妇女瘦素受体基因的Gln223Arg等位基因型、等位基因频率分布无显著性差异(P>0.05)。结论:EMs患者腹腔液中血管生长因子VEGF增高,促进基质降解的基质金属蛋白酶MMP-2增高,而其抑制物TIMP-2降低,这些腹腔微环境的改变有利于内异症的发生和发展。广州地区汉族妇女人群中的LEPR编码基因中存在Gln223Arg的多态性,尚未发现这个多态性在EMs患者中有差异分布。更多还原

【Abstract】 Objective: To measure the levels of matrix metalloproteinases-2 (MMP-2), tissue inhibitor of metalloprotienases (TIMP-2), Vascular endothelial growth factor(VEGF), basic fibroblast growth factor(bFGF) in peritoneal fluid (PF) from patients with endometriosis so as to investigate the roles these cytokines play in the occurence of endometriosis. To study whether the Gln223Arg polymorphism in the leptin receptor (LEPR) gene is associated with endometriosis and their microenvironment changes of abdominal cavity in order to explore the pathogenesis of endometriosis.Methods: Peripheral blood and peritoneal fluid specimens were obtained from 30 cases of endometriosis and 30 cases of non-endometriosis controls, concentrations of BFGF、VEGF、MMP-2、TIMP-2 were examined by enzyme linked immunosorbent assays(ELISA). The Gln223Arg polymorphism was studied by restriction fragment length polymorphism(RFLP)-PCR.Results: The MMP-2 and VEGF concentrations in peritoneal fluids of endometriosis groups were significantly higher than that in controls, while the TIMP-2 concentration was significantly lower than which in controls (P<0.05).The BFGF concentration in peritoneal fluids had no significant difference between these two groups(P>0.05). There were no significant differences on the distributions of genotype and gene frequency of The LEPR Gln223Arg gene between the two groups (P>0.05).Conclusion: The increase of MMP-2、VEGF and the decrease of TIMP-2 in the peritoneal fluid play roles to the occurrence and development of endometriosis. There exists Gln223Arg Polymoprhism of LEPR gene in PoPulation of Han nationality in Guangzhou area, but no difference was found on the distribution of the Polymoprhism in the endometriosis patients.更多还原