【作者】 王烁；

【导师】 程健；

【作者基本信息】 中国科学技术大学 ， 检测技术与自动化装置， 2009， 硕士

【摘要】 生物化学发光免疫分析法(Chemiluminescence Immunoassay, CLIA)是建立在放射免疫分析技术(Radio Immunoassay, RIA)理论的基础上,以标记发光剂为示踪物信号建立起来的一种非放射标记免疫分析法,具有灵敏度高、线性范围宽、易操作、易于实现自动化等优点。经过三十多年的发展,以光电倍增管(Photomultiplier tube, PMT)为核心部件的生物化学发光测量仪已经成为成熟的医疗诊断设备。通用型生物化学发光测量仪设备价格昂贵、体积笨重、功耗巨大,难以普及推广到家庭和个人,而随着近年来光电器件研究的进展,实现生物化学发光测量仪的微型化、便携化具备了一定的条件。本课题是便携式生物化学发光测量仪的预研项目,通过对生物化学发光(Biochemical luminescence, BCL)测量领域的调研和研究,提出了基于固态光电传感器的生物化学发光测量仪的设计方案,设计并研制出了用于实验和验证的生物化学发光测量系统硬件平台,为便携式生物化学发光测量仪的研制,提供了技术基础。本文的工作主要体现在以下几个方面:1.调研了生物化学发光测量与光子测量与计数领域的前沿研究现状,对应用于生物化学发光测量领域的光电倍增管、电荷耦合器件(Charge-Coupled Device,CCD)、雪崩击穿光电二极管(avalanche Photodiode, APD)、硅光电倍增管(Silicon Photomultiplier, SiPM)等光电传感器进行了调研,提出了基于APD与SiPM的便携式生物化学发光测量系统设计方案。2.设计并研制出了基于光电倍增管的前端光子信号测量单元,为生物化学发光测量系统的研究提供了一个实验和验证的传感器平台。3.设计了基于ARM+FPGA的生物化学发光测量系统的后端数据采集与处理平台,完成了底层软件系统的开发。作为后端数据采集与处理的实验与验证平台,设计并实现了基于32位DSP处理器的高速信号采集系统。4.完成了整个生物化学发光测量平台的基础功能测试,为本项目后续的研究工作提供了基本的平台。更多还原

【Abstract】 Based on the theory of RIA technology, chemiluminescence immunoassay is a kind of non-radioactive immunoassay. With advantages of high sensitivity, wide linear range, easy to use and easy to realize automation, chemiluminescence immunoassay uses the marked brightening agent as Signal tracer. After 30 years research and development, it has become a mature immune analysis technique. But the General purpose Chemiluminescence instrument is too expensive, bulky size, great power to promote universal access to families and individuals. Compared to the professional-grade large-scale medical equipment in hospitals, Portable personal medical diagnostic equipment has its advantage of convenience. In recent years,with the progress of optoelectronic devices, luminescence measuring instrument has a certain condition to achieve miniaturization and portable.With the work of investigation and research in the field of chemiluminescence, a forward-looking thinking and design for chemiluminescence detecting system based on solid-state photoelectric sensor was made, a chemiluminescence system hardware platform for Authentication was designed and build. This work provides the basis for the further research of portable-chemiluminescence instrument. The research work of this paper lies in the following areas:1. In the filed of chemiluminescence and photo detecting and counting, the cutting-edge research was studied. Researched several photoelectric sensors such as PMT, CCD, avalanche photodiode (APD), silicon photodiode tube (SiPM), and so on. Give out a portable chemiluminescence detecting system design.2. Designed and developed a front-end module of photon signal acquisition for chemiluminescence detecting system based on the photomultiplier tube. This provides a verification of the sensor platform for the research and development of chemiluminescence instrument.3. Designed and implemented back-end signal processing platform based on ARM + FPGA for biochemiluminescence detecting system, and developed the Underlying software system. Also, Designed and developed a high-speed signal acquisition system based on 32-bit DSP.4. Realized the functional verification of the entire chemiluminesecnce system. The work made a theoretical basis and experimental platform for the further research.更多还原