【作者】 韩静；

【导师】 丁永辉； 宋平顺；

【作者基本信息】 兰州大学 ， 药学， 2009， 硕士

【摘要】 本文参考了有关中药贝母分布、分类及药用资源的研究资料,运用可见-紫外分光光度法及HPLC法进行质量分析、评价及化学成分多样性比较,对贝母种的确定和种的多样性进行探索研究。1.从品种考证、活性成分含量测定、功效及药理作用等方面对贝母进行综述,为进一步开发贝母药用资源提供了基础研究资料。2.通过查阅大量文献资料,从基源和地理分布等方面对中药贝母多样性资料进行研究。国内中药贝母的植物来源比较复杂。除药典收载的贝母植物来源外,各地习用品的植物来源也多种多样。甘肃省作为贝母的一个产区,广泛分布着具有利用价值的种类。经过文献研究,确定甘肃贝母属药用植物有7种。3.采用高效液相色谱法对平贝母、浙贝母中水溶性成分的指纹图谱进行了初步研究。结果表明,平贝母与浙贝母的共有模式具有明显的相似性。平贝母、浙贝母中所含的非共有峰,表明平贝母、浙贝母样品在化学成分组成上是存在多样性,这对于中药贝母鉴别、分类、质量评价有重要的意义。4.采用HPLC-ELSD法同时测定市售平贝母、浙贝母中贝母素甲、贝母素乙的含量,结果表明,贝母素甲、贝母素乙在0.058～0.348μg和0.084～0.504μg范围内线性关系良好。平均回收率(n=6)分别为98.43%,98.87%,RSD分别为1.79%,1.94%。根据测定结果建议平贝母中贝母素甲和贝母素乙的含量总和应不少于0.007%。此法简便,快捷,结果可靠。5.采用酸性染料比色法测定市售平贝母、浙贝母中总生物碱的含量。测定结果表明,浙贝母中总生物碱的含量平均值明显高于平贝母中总生物碱的含量。为进一步全面评价贝母的质量、品种鉴别提供依据。可作为贝母中总生物碱含量测定的实用技术。6.采用高效液相色谱法测定平贝母、浙贝母中腺苷的含量,结果表明,腺苷在0.040～0.240μg与峰面积线性关系良好(r=0.9999),平均加样回收率为96.32%,RSD=1.15%(n=5)。贝母中腺苷的含量比较丰富,平贝母、浙贝母中腺苷的平均含量没有明显的差异。方法简便、准确、重复性好。本论文主要结论为:中药贝母有多方面的多样性,存在较大的潜在价值,应加大对中药贝母资源的研究。1.通过高效液相色谱法进行指纹图谱研究得到平贝母与浙贝母的水溶性成分共有模式具有明显的相似性,在此研究基础上,认为平贝母可以当浙贝母使用。2.中药贝母有两大类:一是川贝母,主要功效是清热润肺、化痰止咳;二是浙贝母,主要功效是清热散结、化痰止咳。其它各类贝母,均分别作为这两种贝母的代用品。平贝母则通常作为川贝母使用。本实验结果则显示,平贝母与浙贝母相似性很好。更多还原

【Abstract】 This article made reference to the research materials of Fritillaria,studied the distribution,classification and medicinal resources of Fritillaria,by using UV spectrophotometry and HPLC method to analyse and evaluate the quality and study of diversity of Bulbus Fritillaria.1.In this paper,we summaried Fritillaria from their varieties of research,the active ingredients,determination,efficacy,Pharmacological effects and other aspects, and supplied a basis research data for further developing of medicinal plants of Fritillaria.2.Through accessing to a large number of documents,we studied biodiversity information of Fritillaria from the original base and geographical distribution.It showed that Plant sources of Fritillaria were complex.As a region,Gansu had certain useful species,after studying the documents,we believed Gansu had 7 kinds of medicinal plants of Fritillaria.3.With HPLC method,did some works on building up fingerprint of water-solubility components in Bulbus Fritillariae.The results showed we could evaluate other sample’s quality with similarity on the basis of standard fingerprint established.According to the non-total peaks in Bulbus Fritillaria,we can see Bulbus Fritillaria exist diversity in the chemical composition,this has a great significance for identification,classification and quality evaluation of traditional Chinese medicine Bulbus Fritillaria.4.Determination of verticine and verticinone in Bulbus Fritillaria by HPLC-ELSD,The calibration curves for verticine and verticinone were linear within the range of 0.058～0.348μg and 0.084～0.504μg respectively.The recoveries were 98.43%and 98.87%with RSD 1.79%and 1.94%(n=6) respectively.Based on the above results,we suggested the sum contents of verticine and verticinone in Bulbus Fritillaria ussuriensis were not less than0.007%,The developed method was simple, rapid and reliable.5.Determination of total alkaloids in Bulbus Fritillaria by Acid dye colorimetry. The results showed that the total alkaloids in Bulbus Fritillaria thunbergii were significantly higher than that in Bulbus Fritillaria ussuriensis.This provided a basis for comprehensively evaluating the quality of Bulbus Fritillaria and for the species identification.This method can be used as a practical technology for the determination of total alkaloids in Bulbus Fritillaria.6.Determination of adenosine in Bulbus Fritillaria by HPLC,The method was simple,accurate and had a good reproducibility.The linear was good in the range of 0.040～0.240μg(r=0.9999)with average recovery of 96.32%and RSD of 1.15%(n= 5).The content of adenosine in Bulbus Fritillaria was relatively rich.The average content of adenosine had not significant difference in the two Chinese herbal medicine.Chief conclusion:Fritillaria is a traditional Chinese medicine,it has a wide range of diversity,the resources of it must be increasely studied.1.With HPLC method,we found the fingerprints of water-solubility components of Fritillariae ussuriensis and Fritillaria thunbergii had obvious similarities.In this research,we think Fritillaria ussuriensis can be used as Fritillaria thunbergii.2.Fritillaria has two categories:the first is Bulbus Fritillaria cirrhosa,the second is Bulbus Fritillaria thunbergii.The other Fritillarias,both as a substitute for the two Fritillarias.Fritillaria ussuriensis is often used as Fritillaria cirrhosa.The results showed Fritillaria ussuriensis and Fritillaria thunbergii had a good similarity.更多还原